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55-580: In general, the reaction mechanism first involves the in situ generation of a hydrazone by condensation of hydrazine with the ketone or aldehyde substrate. Sometimes it is however advantageous to use a pre-formed hydrazone as substrate (see modifications ). The rate determining step of the reaction is de-protonation of the hydrazone by an alkoxide base to form a diimide anion by a concerted, solvent mediated protonation/de-protonation step. Collapse of this alkyldiimide with loss of N 2 leads to formation of an alkylanion which can be protonated by solvent to give

110-463: A diimine intermediate 3 to the corresponding hydrocarbon. A slight variation of this mechanism occurs when tautomerization to the azohydrazone is facilitated by inductive effects . The transient azohydrazine 4 can then be reduced to the tosylhydrazine derivative 2 and furnish the decarbonylated product analogously to the first possibility. This mechanism operates when relatively weak hydride donors are used, such as sodium cyanoborohydride . It

165-407: A carbonyl group can lead to elimination affording unsaturated hydrocarbons under typical reaction conditions. Leonard later further developed this reaction and investigated the influence of different α -substituents on the reaction outcome. He found that the amount of elimination increases with increasing steric bulk of the leaving group. Furthermore, α -dialkylamino-substituted ketones generally gave

220-434: A concerted fashion with a solvent-induced abstraction of the second proton at the nitrogen terminal. Szmant’s finding that this reaction is first order in both hydroxide ion and ketone hydrazone supports this mechanistic proposal. Several molecules of solvent have to be involved in this process in order to allow for a concerted process. A detailed Hammett analysis of aryl aldehydes, methyl aryl ketones and diaryl ketones showed

275-557: A mixture of reduction and elimination product whereas less basic leaving groups resulted in exclusive formation of the alkene product. The fragmentation of α,β -epoxy ketones to allylic alcohols has been extended to a synthetically useful process and is known as the Wharton reaction . Grob rearrangement of strained rings adjacent to the carbonyl group has been observed by Erman and coworkers. During an attempted Wolff–Kishner reduction of trans-π -bromocamphor under Cram’s conditions, limonene

330-532: A non-linear relationship which the authors attribute to the complexity of the rate-determining step. Mildly electron-withdrawing substituents favor carbon-hydrogen bond formation, but highly electron-withdrawing substituents will decrease the negative charge at the terminal nitrogen and in turn favor a bigger and harder solvation shell that will render breaking of the N-H bond more difficult. The exceptionally high negative entropy of activation values observed can be explained by

385-487: A one pot procedure in high yield. [This graphic is wrong. It should be TBS-N, not TBSO-N] The newly developed method was compared directly to the standard Huang–Minlon Wolff–Kishner reduction conditions (hydrazine hydrate, potassium hydroxide, diethylene glycol, 195 °C) for the steroidal ketone shown above. The product was obtained in 79% yield compared to 91% obtained from the reduction via an intermediate N-tert -butyldimethylsilylhydrazone. The Wolff–Kishner reduction

440-512: A reaction also well known using hydrazine hydrate . With a transition metal catalyst , hydrazones can serve as organometallic reagent surrogates to react with various electrophiles. In N , N -dialkylhydrazones the C=N bond can be hydrolysed, oxidised and reduced, the N–N bond can be reduced to the free amine. The carbon atom of the C=N bond can react with organometallic nucleophiles. The alpha-hydrogen atom

495-442: A test to differentiate monosaccharides . Hydrazones are the basis for various analyses of ketones and aldehydes. For example, dinitrophenylhydrazine coated onto a silica sorbent is the basis of an adsorption cartridge. The hydrazones are then eluted and analyzed by high-performance liquid chromatography (HPLC) using a UV detector. The compound carbonyl cyanide- p -trifluoromethoxyphenylhydrazone (abbreviated as FCCP)

550-531: A wide range of functional groups. Huang Minlon Huang Minlon , Huang-Minlon , or Huang Minglong ( simplified Chinese : 黄鸣龙 ; traditional Chinese : 黃鳴龍 ; 3 July 1898 – 1 July 1979) was a Chinese organic chemist and pharmaceutical scientist. Huang is considered a pioneer and founder of modern pharmaceutical industries in China. Huang was born in Yangzhou , Jiangsu Province on 3 July 1898, during

605-493: Is heated for another 3 to 4 h to decompose the hydrazone. Because the second step occurs under nearly anhydrous conditions, yields tend to be higher, while reaction times are sometimes dramatically shortened compared to the original version of the reaction. Even with the development of other variants of the Wolff-Kishner reaction, it remains a widely practiced version of the reaction today. Some other practical advantages include

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660-438: Is known that these sodium cyanoborohydride is not strong enough to reduce imines , but can reduce iminium ions. When stronger hydride donors are used, a different mechanism is operational, which avoids the use of acidic conditions. Hydride delivery occurs to give intermediate 5, followed by elimination of the metal sulfinate to give azo intermediate 6 . This intermediate then decomposes, with loss of nitrogen gas , to give

715-478: Is more acidic by 10 orders of magnitude compared to the ketone and therefore more nucleophilic. Deprotonation with for instance lithium diisopropylamide (LDA) gives an azaenolate which can be alkylated by alkyl halides. The hydrazines SAMP and RAMP function as chiral auxiliary . Several methods are known to recover carbonyl compounds from N,N-dialkylhydrazones. Procedures include oxidative, hydrolytic or reductive cleavage conditions and can be compatible with

770-482: Is not suitable for base–sensitive substrates and can under certain conditions be hampered by steric hindrance surrounding the carbonyl group. Some of the more common side-reactions are listed below. A commonly encountered side-reaction in Wolff–Kishner reductions involves azine formation by reaction of hydrazone with the carbonyl compound. Formation of the ketone can be suppressed by vigorous exclusion of water during

825-467: Is prevented due to torsional restrictions. The product was obtained in 68% overall yield in a two step procedure. A tricyclic carbonyl compound was reduced using the Huang Minlon modification of the Wolff–Kishner reduction. Several attempts towards decarbonylation of tricyclic allylic acetate containing ketone failed and the acetate functionality had to be removed to allow Wolff–Kishner reduction. Finally,

880-536: Is the reduction of the ketone or aldehyde to the corresponding alcohol. After initial hydrolysis of the hydrazone, the free carbonyl derivative is reduced by alkoxide to the carbinol. In 1924, Eisenlohr reported that substantial amounts of hydroxydecalin were observed during the attempted Wolff–Kishner reduction of trans-β -decalone. In general, alcohol formation may be repressed by exclusion of water or by addition of excess hydrazine. Kishner noted during his initial investigations that in some instances, α -substitution of

935-408: Is used to uncouple ATP synthesis and reduction of oxygen in oxidative phosphorylation in molecular biology . Hydrazones are the basis of bioconjugation strategies. Hydrazone-based coupling methods are used in medical biotechnology to couple drugs to targeted antibodies (see ADC ), e.g. antibodies against a certain type of cancer cell. The hydrazone-based bond is stable at neutral pH (in

990-619: The Wolff–Kishner reduction . Hydrazones are reactants in hydrazone iodination , the Shapiro reaction , and the Bamford–Stevens reaction to vinyl compounds. Hydrazones can also be synthesized by the Japp–Klingemann reaction via β-keto acids or β-keto-esters and aryl diazonium salts. Hydrazones are converted to azines when used in the preparation of 3,5-disubstituted 1 H - pyrazoles ,

1045-416: The hydrazone derivative. The Huang-Minlon procedure calls for first heating the carbonyl compound, sodium or potassium hydroxide, and hydrazine hydrate (85% hydrazine) together in ethylene glycol for 1 to 2 h to form the hydrazone before removing the reflux condenser and allowing the water and excess hydrazine to boil off, after which the temperature rises to around 195 °C, and the reaction mixture

1100-425: The α,β -unsaturated tosylhydrazone. The authors predicted that diastereoselective transfer of the diazene hydrogen to one face of the prochiral alkene could be enforced during the suprafacial rearrangement. In 2004, Myers and coworkers developed a method for the preparation of N-tert -butyldimethylsilylhydrazones from carbonyl-containing compounds. These products can be used as a superior alternative to hydrazones in

1155-624: The Chair of Department of Chemistry, Academy of Military Medical Sciences of PLA . Huang was also a senior researcher at the Shanghai Institute of Organic Chemistry (SIOC) of the Chinese Academy of Sciences . Huang is regarded as one of pioneers and founders of modern pharmaceutical industries in China. Huang was a senior academician at the Chinese Academy of Sciences (1955 election). Huang

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1210-570: The UK. Huang returned to China in 1940 and became a senior researcher at Academia Sinica . Huang was also a professor at the renowned wartime National Southwestern Associated University during the Japanese occupation. Subsequently, during 1945-1952, Huang was a visiting professor at Harvard University in the US. Huang was also a visitor at Merck during this time. In 1952, Huang returned to China. He served as

1265-423: The Wolff–Kishner reduction is one of the final steps in their synthesis of (±)-aspidospermidine. The carbonyl group that was reduced in the Wolff–Kishner reduction was essential for preceding steps in the synthesis. The tertiary amide was stable to the reaction conditions and reduced subsequently by lithium aluminum hydride. Amides are usually not suitable substrates for the Wolff–Kishner reduction as demonstrated by

1320-414: The Wolff–Kishner reduction is the collapse of the dimide anion 2 in the presence of a proton source to give the hydrocarbon via loss of dinitrogen to afford an alkyl anion 3 , which undergoes rapid and irreversible acid-base reaction with solvent to give the alkane. Evidence for this high-energy intermediate was obtained by Taber via intramolecular trapping. The stereochemical outcome of this experiment

1375-467: The allylic alcohol was installed via oxyplumbation. The Wolff–Kishner reduction has also been used on kilogram scale for the synthesis of a functionalized imidazole substrate. Several alternative reduction methods were investigated, but all of the tested conditions remained unsuccessful. Safety concerns for a large scale Wolff–Kishner reduction were addressed and a highly optimized procedure afforded to product in good yield. An allylic diazene rearrangement

1430-458: The blood), but is rapidly destroyed in the acidic environment of lysosomes of the cell. The drug is thereby released in the cell, where it exerts its function. Hydrazones are susceptible to hydrolysis: Alkyl hydrazones are 10 - to 10 -fold more sensitive to hydrolysis than analogous oximes . When derived from hydrazine itself, hydrazones condense with a second equivalent of a carbonyl to give azines : Hydrazones are intermediates in

1485-547: The corresponding alkanes is known as the Caglioti reaction. The initially reported reaction conditions have been modified and hydride donors such as sodium cyanoborohydride , sodium triacetoxyborohydride , or catecholborane can reduce tosylhydrazones to hydrocarbons. The reaction proceeds under relatively mild conditions and can therefore tolerate a wider array of functional groups than the original procedure. Reductions with sodium cyanoborohydride as reducing agent can be conducted in

1540-420: The corresponding alkenes with migration of the double bond. The reduction proceeds stereoselectively to furnish the E geometric isomer. A very mild method uses one equivalent of catecholborane to reduce α,β -unsaturated tosylhydrazones. The mechanism of NaBH 3 CN reduction of α,β -unsaturated tosylhydrazones has been examined using deuterium-labeling. Alkene formation is initiated by hydride reduction of

1595-471: The decarbonylation reaction can often fail due to unsuccessful formation of the corresponding tosylhydrazone. This is common for sterically hindered ketones, as was the case for the cyclic amino ketone shown below. Alternative methods of reduction can be employed when formation of the hydrazone fail, including thioketal reduction with Raney nickel or reaction with sodium triethylborohydride . α,β -Unsaturated carbonyl tosylhydrazones can be converted into

1650-492: The desired product. Because the Wolff–Kishner reduction requires highly basic conditions, it is unsuitable for base-sensitive substrates. In some cases, formation of the required hydrazone will not occur at sterically hindered carbonyl groups, preventing the reaction. However, this method can be superior to the related Clemmensen reduction for compounds containing acid-sensitive functional groups such as pyrroles and for high-molecular weight compounds. The Wolff–Kishner reduction

1705-459: The development of Wolff’s procedure, wherein the use of high-boiling solvents such as ethylene glycol and triethylene glycol were implemented to allow for the high temperatures required for the reaction while avoiding the need of a sealed tube. These initial modifications were followed by many other improvements as described below. The mechanism of the Wolff–Kishner reduction has been studied by Szmant and coworkers. According to Szmant's research,

Wolff–Kishner reduction - Misplaced Pages Continue

1760-476: The example above. Coe and coworkers found however that a twisted amide can be efficiently reduced under Wolff–Kishner conditions. The authors explain this observation with the stereoelectronic bias of the substrate which prevents “ anti–Bredt ” iminium ion formation and therefore favors ejection of alcohol and hydrazone formation. The amide functionality in this strained substrate can be considered as isolated amine and ketone functionalities as resonance stabilization

1815-462: The first step in this reaction is the formation of a hydrazone anion 1 by deprotonation of the terminal nitrogen by MOH. If semicarbazones are used as substrates, initial conversion into the corresponding hydrazone is followed by deprotonation. A range of mechanistic data suggests that the rate-determining step involves formation of a new carbon–hydrogen bond at the carbon terminal in the delocalized hydrazone anion. This proton capture takes place in

1870-418: The high degree of organization in the proposed transition state. It was furthermore found that the rate of the reaction depends on the concentration of the hydroxylic solvent and on the cation in the alkoxide catalyst. The presence of crown ether in the reaction medium can increase the reactivity of the hydrazone anion 1 by dissociating the ion pair and therefore enhance the reaction rate. The final step of

1925-500: The higher reactivity in DMSO as solvent to higher base strength of potassium tert -butoxide in this medium. This modification has not been exploited to great extent in organic synthesis due to the necessity to isolate preformed hydrazone substrates and to add the hydrazone over several hours to the reaction mixture. Henbest extended Cram’s procedure by refluxing carbonyl hydrazones and potassium tert -butoxide in dry toluene. Slow addition of

1980-447: The hydrazone is not necessary and it was found that this procedure is better suited for carbonyl compounds prone to base-induced side reactions than Cram's modification. It has for example been found that double bond migration in α,β -unsaturated enones and functional group elimination of certain α -substituted ketones are less likely to occur under Henbest's conditions. Treatment of tosylhydrazones with hydride-donor reagents to obtain

2035-441: The iminium ion followed by double bond migration and nitrogen extrusion which occur in a concerted manner. Allylic diazene rearrangement as the final step in the reductive 1,3-transposition of α,β -unsaturated tosylhydrazones to the reduced alkenes can also be used to establish sp -stereocenters from allylic diazenes containing prochiral stereocenters. The influence of the alkoxy stereocenter results in diastereoselective reduction of

2090-640: The late Qing dynasty . In 1917, Huang graduated from Yangzhou Middle School. In 1918, Huang graduated from the Zhejiang Provincial College of Medicine (current Zhejiang University School of Medicine). In 1924, Huang obtained PhD from the University of Berlin , Germany. In 1925, Huang went back to China and became a professor and later department head at Zhejiang Provincial College of Medicine. From 1934 to 1940, Huang worked in research in Germany and

2145-538: The presence of esters, amides, cyano-, nitro- and chloro-substituents. Primary bromo- and iodo-substituents are displaced by nucleophilic hydride under these conditions. Thereduction pathway is sensitive to the pH, the reducing agent, and the substrate. One possibility, occurring under acidic conditions, includes direct hydride attack of iminium ion 1 following prior protonation of the tosylhydrazone. The resulting tosylhydrazine derivative 2 subsequently undergoes elimination of p -toluenesulfinic acid and decomposes via

2200-496: The problems that normally arise with hindered ketones can be alleviated—for example, the C 11 -carbonyl group in the steroidal compound shown below was successfully reduced under Barton’s conditions while Huang–Minlon conditions failed to effect this transformation. Slow addition of preformed hydrazones to potassium tert -butoxide in DMSO as reaction medium instead of glycols allows hydrocarbon formation to be conducted successfully at temperatures as low as 23 °C. Cram attributed

2255-499: The reaction temperature. Some of the newer modifications provide more significant advances and allow for reactions under considerably milder conditions. The table shows a summary of some of the modifications that have been developed since the initial discovery. In 1946, Huang Minlon reported a modified procedure for the Wolff–Kishner reduction of ketones in which excess hydrazine and water were removed by distillation after hydrazone formation. The temperature-lowering effect of water that

Wolff–Kishner reduction - Misplaced Pages Continue

2310-432: The reaction. Several of the presented procedures require isolation of the hydrazone compound prior to reduction. This can be complicated by further transformation of the product hydrazone to the corresponding hydrazine during product purification. Cram found that azine formation is favored by rapid addition of preformed hydrazones to potassium tert -butoxide in anhydrous dimethylsulfoxide. The second principal side reaction

2365-423: The reduced compound. When strongly basic hydride donors are used such as lithium aluminium hydride , then deprotonation of the tosyl hydrazone can occur before hydride delivery. Intermediate anion 7 can undergo hydride attack, eliminating a metal sulfinate to give azo anion 8 . This readily decomposes to carbanion 9 , which is protonated to give the reduced product. As with the parent Wolff–Kishner reduction,

2420-502: The reduction of β -( p -phenoxybenzoyl)propionic acid to γ -( p -phenoxyphenyl)butyric acid in 95% yield compared to 48% yield obtained by the traditional procedure. Nine years after Huang Minlon’s first modification, Barton developed a method for the reduction of sterically hindered carbonyl groups. This method features rigorous exclusion of water, higher temperatures, and longer reaction times as well as sodium in diethylene glycol instead of alkoxide base. Under these conditions, some of

2475-413: The replacement of the oxygen =O with the = N−NH 2 functional group . They are formed usually by the action of hydrazine on ketones or aldehydes. Hydrazine, organohydrazines, and 1,1-diorganohydrazines react with aldehydes and ketones to give hydrazones. Phenylhydrazine reacts with reducing sugars to form hydrazones known as osazones , which was developed by German chemist Emil Fischer as

2530-492: The simple experimental setup, inexpensive starting materials, and a reduced amount of solvent needed, factors which made the conditions suitable for use in China at the time, where chemical supplies were hard to come by. Huang devised this modified synthesis in 1945 while he was in the United States as a visiting professor at Harvard. Due to its simplicity, shortened reaction times, and generally good yield, Huang's modification

2585-432: The transformation of ketones into alkanes. The advantages of this procedure are considerably milder reaction conditions and higher efficiency as well as operational convenience. The condensation of 1,2-bis( tert -butyldimethylsilyl)-hydrazine with aldehydes and ketones with Sc(OTf) 3 as catalyst is rapid and efficient at ambient temperature. Formation and reduction of N-tert -butyldimethylsilylhydrazones can be conducted in

2640-483: The unorthodox spelling of his name as "Huang-Minlon" (making no indication of whether this was a given or family name) in the original reports of his findings, his name is often mistakenly thought to refer to two individuals. The Huang modification is a one-pot shortcut for the Wolff-Kishner reduction , a reaction in which ketone and aldehyde carbonyls are converted to the corresponding methylene or methyl groups via

2695-406: Was discovered independently by N. Kishner in 1911 and Ludwig Wolff in 1912. Kishner found that addition of pre-formed hydrazone to hot potassium hydroxide containing crushed platinized porous plate led to formation of the corresponding hydrocarbon. A review titled “Disability, Despotism, Deoxygenation—From Exile to Academy Member: Nikolai Matveevich Kizhner” describing the life and work of Kishner

2750-464: Was isolated as the only product. Similarly, cleavage of strained rings adjacent to the carbonyl group can occur. When 9 β ,19-cyclo-5 α -pregnane-3,11,20-trione 3,20-diethylene ketal was subjected to Huang–Minlon conditions, ring-enlargement was observed instead of formation of the 11-deoxo-compound. The Wolff–Kishner reduction has been applied to the total synthesis of scopadulcic acid B, aspidospermidine and dysidiolide. The Huang Minlon modification of

2805-427: Was more consistent with an alkyl anion intermediate than the alternative possibility of an alkyl radical. The overall driving force of the reaction is the evolution of nitrogen gas from the reaction mixture. Many of the efforts devoted to improve the Wolff–Kishner reduction have focused on more efficient formation of the hydrazone intermediate by removal of water and a faster rate of hydrazone decomposition by increasing

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2860-549: Was produced in hydrazone formation usually resulted in long reaction times and harsh reaction conditions even if anhydrous hydrazine was used in the formation of the hydrazone. The modified procedure consists of refluxing the carbonyl compound in 85% hydrazine hydrate with three equivalents of sodium hydroxide followed by distillation of water and excess hydrazine and elevation of the temperature to 200 °C. Significantly reduced reaction times and improved yields can be obtained using this modification. Minlon's original report described

2915-420: Was published in 2013. Wolff later accomplished the same result by heating an ethanol solution of semicarbazones or hydrazones in a sealed tube to 180 °C in the presence of sodium ethoxide. The method developed by Kishner has the advantage of avoiding the requirement of a sealed tube, but both methodologies suffered from unreliability when applied to many hindered substrates. These disadvantages promoted

2970-592: Was the Vice-president and later became the Honorary-president of the Chinese Society for Pharmaceutical Sciences (a.k.a. Chinese Pharmaceutical Association). Huang published more than 100 papers, in both English and Chinese. The Huang modification or Huang-Minlon modification is named after Huang Minlon, the earliest instance of an organic reaction associated with the name of a Chinese chemist. Due to

3025-453: Was used in the synthesis of the C 21 –C 34 fragment of antascomicin B. The hydrazone was reduced selectively with catecholborane and excess reducing agent decomposed with sodium thiosulfate. The crude reaction product was then treated with sodium acetate and to give the 1,4- syn isomer. Hydrazone Hydrazones are a class of organic compounds with the structure R R C=N−NH 2 . They are related to ketones and aldehydes by

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