The Coat Protein Complex II , or COPII , is a group of proteins that facilitate the formation of vesicles to transport proteins from the endoplasmic reticulum to the Golgi apparatus or endoplasmic-reticulum–Golgi intermediate compartment . This process is termed anterograde transport , in contrast to the retrograde transport associated with the COPI complex. COPII is assembled in two parts: first an inner layer of Sar1, Sec23, and Sec24 forms; then the inner coat is surrounded by an outer lattice of Sec13 and Sec31.
13-467: SEC31 is a protein which in yeast promotes the formation of COPII transport vesicles from the Endoplasmic Reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules. Its human homologs are SEC31A and SEC31B . COPII The COPII coat is responsible for the formation of vesicles from
26-561: A given location, so it can more efficiently activate Sar1. In mammals there are two Sar1 genes: SAR1A and SAR1B ( SAR1B was previously known as SARA2 ). In cultured mammalian cells the two Sar1 genes appear redundant; however, in animals SAR1B is uniquely required for the formation of large (over 1 micrometer across) COPII-coated vesicles. Similarly, mammals express two Sec23 genes: SEC23A and SEC23B . The two Sec23 isoforms have identical function but are expressed in different body tissues. Both Sec23 proteins can interact with any of
39-468: Is a rare genetic anemia in humans characterized by hereditary erythroblastic multinuclearity with positive acidified serum lysis test. CDA type II is caused by mutations in the SEC23B gene. This gene provides instructions for making a protein that is involved in the transport of other proteins within cells. During the development of red blood cells, this protein may help ensure that proteins are transported to
52-641: Is found to be necessary for packaging glycosylated pro-α-factor. Sec24 proteins recognize various cargo proteins, packaging them into the budding vesicles. The COPII coat consists of an inner layer – a flexible meshwork of Sar1, Sec23, and Sec24 – and an outer layer made of Sec13 and Sec31. Sar1 resembles other Ras-family GTPases, with a core of six beta strands flanked by three alpha helices , and two flexible "switch domains". Unlike other Ras GTPases, Sar1 inserts into membranes via an N-terminal helix (rather than myristoylation or prenylation ). These coat proteins are necessary but insufficient to direct or dock
65-402: Is lethal to mice in utero . In humans, a Sec23A variant causes Cranio-lenticulo-sutural dysplasia , while Sec23B variants are associated with the bone marrow disease congenital dyserythropoietic anemia type II and some cancers . Mice without Sec23B die soon after birth. Halperin-Birk syndrome (HLBKS), a rare autosomal recessive neurodevelopmental disorder, is caused by a null mutation in
78-765: The SEC31A. CopII has three specific binding sites that can each be complexed. The adjacent picture (Sed5) uses the Sec22 t-SNARE complex to bind. This site is more strongly bound, and therefore is more favored. (Embo) Mutations the threonine at position 39 to asparagine generates a dominant negative Sar1A bound permanently to GDP; mutating histidine 79 to glycine generates a constitutively active Sar1A, with GTP hydrolysis slowed dramatically. Congenital dyserythropoietic anemia type II Congenital dyserythropoietic anemia type II (CDA II), or hereditary erythroblastic multinuclearity with positive acidified serum lysis test (HEMPAS)
91-450: The areas where they are needed. Researchers are working to determine how mutations in the SEC23B gene lead to the signs and symptoms of CDA type II. Analyses of CDA II erythrocyte membranes showed that the band 3 glycoprotein is underglycosylated. An aberrant glycosylation pattern is seen in the polylactosamine carbohydrates which are normally attached to the band 3 and band 4.5 glycoproteins. The polylactosamines are, however, accumulated in
104-510: The cuboctahedron deforms the ER membrane and detaches the COPII vesicle (alongside cargo proteins and v-SNAREs), completing the COPII vesicle budding process. The signal(s) that triggers Sec12 to initiate COPII assembly remains unclear, though some regulators of coat formation are now known. The frequency of COPII formation is regulated in part by Sec16A and Tango1 proteins, likely by concentrating Sec12 in
117-504: The endoplasmic reticulum (ER). These vesicles transport cargo proteins to the Golgi apparatus (in yeast) or the endoplasmic-reticulum-Golgi intermediate compartment (ERGIC, in mammals). Coat assembly is initiated when the cytosolic Ras GTPase Sar1 is activated by its guanine nucleotide exchange factor Sec12. Activated Sar1-GTP inserts itself into the ER membrane, binding preferentially to areas of membrane curvature. As Sar1-GTP inserts into
130-599: The form of glycolipids. Therefore a genetic factor in CDA II appears to block the glycosylation of protein acceptors and shift these carbohydrates to the lipid acceptors. Structural analysis of CDA II band 3 carbohydrates identified truncated hybrid-type oligosaccharides and suggests that the Golgi glycosylation enzyme(s), alpha-mannosidase II or N-acetylglycosaminyltransferase II is defective in CDA II. The anemia associated with CDA type II can range from mild to severe, and most affected individuals have jaundice, hepatosplenomegaly, and
143-551: The four Sec24 proteins: SEC24A, SEC24B, SEC24C, and SEC24D. Lethal or pathogenic variants of most COPII proteins have been described. Loss of Sar1B in mice results in death soon after birth. In humans, inheriting two copies of certain SAR1B variants results in Chylomicron retention disease , and loss of Sar1B causes a combination of chylomicron retention disease and the neuromuscular disorder Marinesco–Sjögren syndrome . Loss of Sec23A
SECTION 10
#1732952548424156-655: The membrane, it recruits Sec23 and Sec24 to make up the inner cage. Once the inner coat is assembled, the outer coat proteins Sec13 and Sec31 are recruited to the budding vesicle. Hydrolysis of the Sar1 GTP to GDP promotes disassembly of the coat. Some proteins are found to be responsible for selectively packaging cargos into COPII vesicles. More recent research suggests the Sec23/Sec24-Sar1 complex participates in cargo selection. For example, Erv29p in Saccharomyces cerevisiae
169-464: The vesicle to the correct target membrane. SNARE , cargo, and other proteins are also needed for these processes to occur. Pre-budding complex (composed of Sar1-GTP and Sec23/24) recruits the flexible Sec13p/31p complex, characterized by polymerization of the Sec13/31 complex with other Sec13/31 complexes to form a cuboctahedron with a broader lattice than its Clathrin vesicle analog. The formation of
#423576