Cable television first became available in the United States in 1948. By 1989, 53 million U.S. households received cable television subscriptions, with 60 percent of all U.S. households doing so in 1992. Most cable viewers in the U.S. reside in the suburbs and tend to be middle class ; cable television is less common in low income , urban , and rural areas.
171-519: The Oprah Winfrey Network ( OWN ) is an American multinational basic cable television network which launched on January 1, 2011, effectively replacing the Discovery Health Channel . OWN (also known as the “OWN Network”) is a joint venture between Warner Bros. Discovery and Harpo Studios , conceptualized largely by popular talk show host, Oprah Winfrey —the name of Harpo Studios is Winfrey’s first name, spelled backwards. The network
342-400: A "major stake" in the push towards more diverse portrayals of African Americans in television, while Iyanla Vanzant argued that OWN had been "celebrating the unseen" among African Americans with shows that "give the minds of the viewers a different interpretation than the one that's often put out in the world." In October 2018, network president Erik Logan announced that he would step down at
513-524: A 50/50 joint venture, Discovery acquired a larger stake in the network in 2017 and again in December 2020, when Discovery increased its ownership in OWN from 73% to 95%. Harpo remains a "significant" minority stakeholder, and Winfrey is contracted with the channel through (at least) 2025. As of February 2015, OWN is available to approximately 81.9 million pay television households (70.3% of households with television) in
684-610: A VHS tape (although some on demand services, generally those offered by broadcast networks, restrict the ability to fast forward through a program). Some of the offerings have a cost similar to renting a movie at a video store while others are free. On-demand content has slowly been replacing traditional pay-per-view for pre-recorded content; pay-per-view remains popular for live combat sports events (boxing, mixed martial arts and professional wrestling). Additional subscription fees are also usually required to receive digital cable channels. Many cable systems operate as de facto monopolies in
855-505: A broadcasters' convention. In the spring of 1948, Parsons learned that radio station KRSC (now KKNW ) in Seattle – 125 miles away – was going to launch a television station that fall. He found that with a large antenna he could receive KRSC's signal on the roof of the Hotel Astoria and from there he ran coaxial cable across the street to his apartment. When the station (now KING-TV ) went on
1026-445: A buffer to recruit or titrate ions or antibiotics. Extracellular DNA acts as a functional extracellular matrix component in the biofilms of several bacterial species. It may act as a recognition factor to regulate the attachment and dispersal of specific cell types in the biofilm; it may contribute to biofilm formation; and it may contribute to the biofilm's physical strength and resistance to biological stress. Cell-free fetal DNA
1197-459: A cell makes up its genome ; the human genome has approximately 3 billion base pairs of DNA arranged into 46 chromosomes. The information carried by DNA is held in the sequence of pieces of DNA called genes . Transmission of genetic information in genes is achieved via complementary base pairing. For example, in transcription, when a cell uses the information in a gene, the DNA sequence is copied into
1368-450: A chain by covalent bonds (known as the phosphodiester linkage ) between the sugar of one nucleotide and the phosphate of the next, resulting in an alternating sugar-phosphate backbone . The nitrogenous bases of the two separate polynucleotide strands are bound together, according to base pairing rules (A with T and C with G), with hydrogen bonds to make double-stranded DNA. The complementary nitrogenous bases are divided into two groups,
1539-493: A co-executive producer of The Oprah Winfrey Show since 2006—was named chief creative officer of OWN. Due to her history of working with Winfrey, the appointment of Erspamer was seen as having stabilized OWN's development; network CEO Christina Norman described Erspamer's arrival as being an "injection of Oprah's DNA " into the upcoming channel. Later on November 20, Winfrey officially announced that The Oprah Winfrey Show would conclude in 2011, after its 25th and final season. With
1710-524: A co-president. As of September 2011, Discovery had invested $ 254 million in programming for OWN. The October 2011 premiere of Welcome to Sweetie Pie's brought notable gains to the fledgling channel: it was the network's highest-rated series to date, and boosted OWN's average primetime viewership to 216,000. The show was especially popular among African American audiences, which prompted OWN's executives to factor this demographic into its programming decisions and advertising sales going forward. October 16 saw
1881-445: A complementary RNA sequence through the attraction between the DNA and the correct RNA nucleotides. Usually, this RNA copy is then used to make a matching protein sequence in a process called translation , which depends on the same interaction between RNA nucleotides. In an alternative fashion, a cell may copy its genetic information in a process called DNA replication . The details of these functions are covered in other articles; here
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#17328512207562052-492: A double helix can thus be pulled apart like a zipper, either by a mechanical force or high temperature . As a result of this base pair complementarity, all the information in the double-stranded sequence of a DNA helix is duplicated on each strand, which is vital in DNA replication. This reversible and specific interaction between complementary base pairs is critical for all the functions of DNA in organisms. Most DNA molecules are actually two polymer strands, bound together in
2223-536: A few years, only to fail due to the inability to compete against established premium services that had broader distribution and higher subscriber totals. Since cable television channels are not broadcast on public spectrum, they are not subject to FCC regulations on indecent material. Premium networks generally offer broader portrayal of profanity, sex and violence; some premium services–such as Cinemax and The Movie Channel (which have carried such programs as part of their late-night schedules) as well as Playboy TV , one of
2394-477: A final decision as to whether she will continue her show in syndication beyond [2011]". The development of OWN was affected by internal conflicts, as well as Winfrey's continued commitment to her talk show. Its launch was pushed back from its originally-announced target of 2009 to an unspecified date. Zaslav pressured Oprah into moving her talk show to the channel, believing that it could boost its business. In November 2009, Lisa Erspamer—a Harpo executive who had been
2565-479: A focus on more "mature" and creator-driven series to help attract critical acclaim and key demographic viewership. Turner Classic Movies has aired uncut and commercial-free prints of theatrical films that have featured nudity, sexual content, violence and profanity, as had the now-ad-supported SundanceTV and IFC , the former of which began as a premium service, spun off from Showtime. Commercial-free basic channels have tended to rate their film presentations using
2736-428: A full set of the mitochondrial genes. Each human mitochondrion contains, on average, approximately 5 such mtDNA molecules. Each human cell contains approximately 100 mitochondria, giving a total number of mtDNA molecules per human cell of approximately 500. However, the amount of mitochondria per cell also varies by cell type, and an egg cell can contain 100,000 mitochondria, corresponding to up to 1,500,000 copies of
2907-597: A group of fellow television set retailers in Lansford, Pennsylvania , a town in the same region as Mahanoy City, to offer television signals from Philadelphia, Pennsylvania broadcast stations to homes in Lansford for a fee. The system was featured in stories in The New York Times , Newsweek and The Wall Street Journal . The publicity of this successful early system set off a wave of cable system construction throughout
3078-439: A helical fashion by noncovalent bonds; this double-stranded (dsDNA) structure is maintained largely by the intrastrand base stacking interactions, which are strongest for G,C stacks. The two strands can come apart—a process known as melting—to form two single-stranded DNA (ssDNA) molecules. Melting occurs at high temperatures, low salt and high pH (low pH also melts DNA, but since DNA is unstable due to acid depurination, low pH
3249-571: A higher number is also possible but this would be against the natural principle of least effort . The phosphate groups of DNA give it similar acidic properties to phosphoric acid and it can be considered as a strong acid . It will be fully ionized at a normal cellular pH, releasing protons which leave behind negative charges on the phosphate groups. These negative charges protect DNA from breakdown by hydrolysis by repelling nucleophiles which could hydrolyze it. Pure DNA extracted from cells forms white, stringy clumps. The expression of genes
3420-451: A higher quality picture than their analog counterparts. This is often true, with a dramatic improvement in chroma resolution (120 lines for NTSC versus 270 for digital). However, digital compression has a tendency to soften the quality of the television picture, particularly of channels that are more heavily compressed. Pixelation and other artifacts are often visible. Subscribers wishing to have access to digital cable channels must have
3591-573: A joint venture with another partner. Zaslav's wife was an avid reader of Oprah Winfrey's O magazine (a joint venture with Hearst Corporation ); believing that her values could serve as the basis for a cable network, he contacted Winfrey's agents to hold a meeting in April 2007. On January 15, 2008, Discovery Communications officially announced that it had entered into a joint venture with Winfrey's studio Harpo Productions , under which it would re-launch Discovery Health as "OWN: The Oprah Winfrey Network", in
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#17328512207563762-401: A large audience; its two-night premiere event was seen by 1.1 and 1.6 million total viewers respectively. The Sunday-night airing was the third-highest-rated program of the night across ad-supported cable networks, and was the highest-rated program of the night among women 18-49 and African American women 25–54. By October 2012, OWN had seen increases in audience share, especially among women in
3933-555: A less expensive entry point into the cable marketplace. Some cable/satellite providers might wish to sell channels à la carte, but their contracts with programmers often require the more standardized approach. Starting in the late 1990s, advances in digital signal processing (primarily Motorola's DigiCipher 2 video compression technology in North America) gave rise to wider implementation of digital cable services. Digital cable television provides many more television channels over
4104-401: A local station if the duplication is shown either 15 days before or after its local airing. This 1965 report reasoning is as follows: 1) CATV should carry local stations because CATV supplements, not replaces, local stations; and, the non-carriage of local stations gives distant stations an advantage since people will not change from the cable to the antenna to see a local station; 2) non-carriage
4275-667: A long-standing puzzle known as the " C-value enigma ". However, some DNA sequences that do not code protein may still encode functional non-coding RNA molecules, which are involved in the regulation of gene expression . Some noncoding DNA sequences play structural roles in chromosomes. Telomeres and centromeres typically contain few genes but are important for the function and stability of chromosomes. An abundant form of noncoding DNA in humans are pseudogenes , which are copies of genes that have been disabled by mutation. These sequences are usually just molecular fossils , although they can occasionally serve as raw genetic material for
4446-557: A mix of per-subscriber carriage fees paid by the provider, and revenue from advertising sold on the service, as their sources of revenue. One of the first "basic cable" networks was TBS —which was initially established as a satellite uplink of an independent television station (the present-day WPCH-TV ) in Atlanta, Georgia. TBS would serve as the starting point for other major basic cable ventures by its owner, Ted Turner , including CNN —the first 24-hour news channel . Another early network
4617-430: A monthly fee depending on the number and perceived quality of the channels offered. Cable television subscribers are offered various packages of channels one can subscribe to. The cost of each package depends on the type of channels offered (basic vs. premium) and the quantity. These fees cover the fees paid to individual cable channels for the right to carry their programming, as well as the cost of operating and maintaining
4788-518: A morning block of talk shows including Dr. Phil , Rachael Ray , and The Nate Berkus Show . The programming includes a mix of original programs, specials, documentaries, and acquired movies. The channel does not air infomercials . In October 2012, OWN expanded into scripted programming by announcing an exclusive development deal with Tyler Perry , beginning with The Haves and the Have Nots and Love Thy Neighbor . In February 2013, OWN ordered
4959-409: A narrower, deeper major groove. The A form occurs under non-physiological conditions in partly dehydrated samples of DNA, while in the cell it may be produced in hybrid pairings of DNA and RNA strands, and in enzyme-DNA complexes. Segments of DNA where the bases have been chemically modified by methylation may undergo a larger change in conformation and adopt the Z form . Here, the strands turn about
5130-442: A premium service to sample its programming, in an effort for subscribers to the participant provider to consider obtaining a subscription to the offered service to continue viewing it following the preview period. HBO was the first true premium cable (or "pay-cable") network as well as the first television network intended for cable distribution on a regional or national basis; however, there were notable precursors to premium cable in
5301-442: A radius of 10 Å (1.0 nm). According to another study, when measured in a different solution, the DNA chain measured 22–26 Å (2.2–2.6 nm) wide, and one nucleotide unit measured 3.3 Å (0.33 nm) long. The buoyant density of most DNA is 1.7g/cm . DNA does not usually exist as a single strand, but instead as a pair of strands that are held tightly together. These two long strands coil around each other, in
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5472-400: A scheduled time (this is generally the main place where pornographic content airs on American cable). Some cable systems have begun to offer on-demand programming , where customers can select programs from a list of offerings including recent releases of movies, concerts, sports, first-run television shows and specials and start the program whenever they wish, as if they were watching a DVD or
5643-416: A second protein when read in the opposite direction along the other strand. In bacteria , this overlap may be involved in the regulation of gene transcription, while in viruses, overlapping genes increase the amount of information that can be encoded within the small viral genome. DNA can be twisted like a rope in a process called DNA supercoiling . With DNA in its "relaxed" state, a strand usually circles
5814-438: A second season), and Season 25: Oprah Behind The Scenes . Its average viewership was 135,000 during its second month on air, which was lower than that of Discovery Health. Norman defended the network's ratings, stating that first-run broadcasts of its original series were drawing larger audiences than those of Discovery Health, and that Discovery's goal was to improve OWN's monthly audience to between 50 and 60 million viewers by
5985-595: A second signal to two of the towns and add two signals to a previously unserved town. A television station in one town opposed this and protested to the FCC on the grounds of economic damage. A hearing examiner supported Carter Mountain, but the Commission supported the television station. The case was taken to appeal, and the Federal Communications Commission won. "The fact that no broadcaster has actually gone off
6156-537: A secretary of the Federal Communications Commission, sent a letter to Parsons requesting that he "furnish [to] the Commission full information with respect to the nature of the system you may have developed and may be operating." This is the first known involvement of the FCC in CATV. An FCC lawyer, E. Stratford Smith, determined the Commission could exercise common carrier jurisdiction over CATV. The FCC did not act on this opinion, and Smith later changed his mind after working in
6327-453: A significant minority shareholder, and Winfrey remaining CEO. Winfrey also extended her exclusivity agreement with the network and Discovery through 2025. In April 2018, OWN ordered two new series from Will Packer —the drama Ambitions and the dating show Ready to Love . In July 2018, Winfrey signed a non-exclusive, multi-platform content deal with Apple Inc. ; it is not expected to affect her role at OWN. Wired considered OWN to have had
6498-431: A significant reduction in subscription fees and advertising revenue, and potentially be driven out of business. Many cable/satellite providers are therefore reluctant to introduce an à la carte business model. They fear it will reduce the overall choice of viewing content, making their service less appealing to customers. Some believe the à la carte option could actually increase overall sales by allowing potential subscribers
6669-445: A simple TTAGGG sequence. These guanine-rich sequences may stabilize chromosome ends by forming structures of stacked sets of four-base units, rather than the usual base pairs found in other DNA molecules. Here, four guanine bases, known as a guanine tetrad , form a flat plate. These flat four-base units then stack on top of each other to form a stable G-quadruplex structure. These structures are stabilized by hydrogen bonding between
6840-453: A small number of national cable networks in their basic lineups. Most systems differentiate between basic cable, which has locals, home shopping channels and local-access television channels, and expanded basic (or "standard"), which carries most of the better-known national cable networks. Most basic cable lineups have approximately 20 channels overall, while expanded basic has channel capacity for as many as 70 channels. Under U.S. regulations,
7011-503: A special cable converter box , (or, more recently, a "Digital Cable Ready" television) and a CableCARD to receive them. AllVid is a CableCARD replacement proposed by the U.S. Federal Communications Commission (FCC), U.S.A Federal Bureau of Investigation (FBI), intended to provide bidirectional compatibilities such as interactive programming guides, video-on-demand and pay-per-view, since retail CableCARD-ready devices are unable to access such systems. Cable television systems impose
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7182-564: A surprise visit when he visited the network to pitch the series) "from the start was wanting to open the story up and understand what it was about". OWN cancelled The Paynes in August 2019. On August 20, 2021, it was reported that OWN was in negotiations to pick up the canceled CBS legal drama All Rise . On September 29, 2021, the series was renewed for a third season scheduled to premiere in 2022; OWN acquired linear rights, while HBO Max and Hulu jointly acquiried streaming rights. In Canada,
7353-522: A third season of Perry's canceled TBS sitcom For Better or Worse . In January 2014, OWN ordered another drama from Perry, If Loving You Is Wrong , which was adapted from Perry's feature film The Single Moms Club . In February 2015, OWN ordered new seasons of For Better Or Worse , The Haves and the Have Nots , If Loving You Is Wrong , and Love Thy Neighbor . In November 2017, OWN announced premiere dates for The Paynes (a spin-off of Perry's TBS sitcom Tyler Perry's House of Payne ), as well as
7524-517: Is "inherently contrary to the public interest"; and, 3) CATV duplication of local programming via distant signals is unfair since broadcasters and CATV do not compete for programs on an equal footing; the FCC recommended "a reasonable measure of exclusivity". The 1966 Second Report and Order made some minor changes in the First Report and Order and added a major regulation. This was designed to protect UHF stations in large cities. The new rule disallowed
7695-413: Is a polymer composed of two polynucleotide chains that coil around each other to form a double helix . The polymer carries genetic instructions for the development, functioning, growth and reproduction of all known organisms and many viruses . DNA and ribonucleic acid (RNA) are nucleic acids . Alongside proteins , lipids and complex carbohydrates ( polysaccharides ), nucleic acids are one of
7866-447: Is called intercalation . Most intercalators are aromatic and planar molecules; examples include ethidium bromide , acridines , daunomycin , and doxorubicin . For an intercalator to fit between base pairs, the bases must separate, distorting the DNA strands by unwinding of the double helix. This inhibits both transcription and DNA replication, causing toxicity and mutations. As a result, DNA intercalators may be carcinogens , and in
8037-435: Is called a polynucleotide . The backbone of the DNA strand is made from alternating phosphate and sugar groups. The sugar in DNA is 2-deoxyribose , which is a pentose (five- carbon ) sugar. The sugars are joined by phosphate groups that form phosphodiester bonds between the third and fifth carbon atoms of adjacent sugar rings. These are known as the 3′-end (three prime end), and 5′-end (five prime end) carbons,
8208-434: Is dependent on ionic strength and the concentration of DNA. As a result, it is both the percentage of GC base pairs and the overall length of a DNA double helix that determines the strength of the association between the two strands of DNA. Long DNA helices with a high GC -content have more strongly interacting strands, while short helices with high AT content have more weakly interacting strands. In biology, parts of
8379-411: Is influenced by how the DNA is packaged in chromosomes, in a structure called chromatin . Base modifications can be involved in packaging, with regions that have low or no gene expression usually containing high levels of methylation of cytosine bases. DNA packaging and its influence on gene expression can also occur by covalent modifications of the histone protein core around which DNA is wrapped in
8550-432: Is introduced by enzymes called topoisomerases . These enzymes are also needed to relieve the twisting stresses introduced into DNA strands during processes such as transcription and DNA replication . DNA exists in many possible conformations that include A-DNA , B-DNA , and Z-DNA forms, although only B-DNA and Z-DNA have been directly observed in functional organisms. The conformation that DNA adopts depends on
8721-679: Is not clear how this might affect subscription costs over all, but it would allow a parent to censor their child's viewing habits by removing any channel they deem objectionable from their subscription. Offering such individualized subscriptions would have been relatively complicated and labor-intensive using analog cable, but the widespread adoption of digital cable & IPTV technologies have now made it more feasible. Analog technology allowed cable providers to offer standardized subscription packages using low-pass filters and notch filters . A low-pass filter lets lower frequency signals pass while removing higher frequency signals. Using such filtering,
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#17328512207568892-422: Is nothing special about the four natural nucleobases that evolved on Earth. On the other hand, DNA is tightly related to RNA which does not only act as a transcript of DNA but also performs as molecular machines many tasks in cells. For this purpose it has to fold into a structure. It has been shown that to allow to create all possible structures at least four bases are required for the corresponding RNA , while
9063-432: Is one of four types of nucleobases (or bases ). It is the sequence of these four nucleobases along the backbone that encodes genetic information. RNA strands are created using DNA strands as a template in a process called transcription , where DNA bases are exchanged for their corresponding bases except in the case of thymine (T), for which RNA substitutes uracil (U). Under the genetic code , these RNA strands specify
9234-514: Is rarely used). The stability of the dsDNA form depends not only on the GC -content (% G,C basepairs) but also on sequence (since stacking is sequence specific) and also length (longer molecules are more stable). The stability can be measured in various ways; a common way is the melting temperature (also called T m value), which is the temperature at which 50% of the double-strand molecules are converted to single-strand molecules; melting temperature
9405-428: Is recreated by an enzyme called DNA polymerase . This enzyme makes the complementary strand by finding the correct base through complementary base pairing and bonding it onto the original strand. As DNA polymerases can only extend a DNA strand in a 5′ to 3′ direction, different mechanisms are used to copy the antiparallel strands of the double helix. In this way, the base on the old strand dictates which base appears on
9576-542: Is spearheaded by television personality (and namesake) Oprah Winfrey and features entertainment, self-help and lifestyle specials aimed at not only African American audiences but female viewers, in general. As well as featuring various programs surrounding celebrities, self-awareness, therapy, and spirituality, the OWN network also airs reruns of talk show programming from the Harpo Studios library, such as archived episodes of The Oprah Winfrey Show (1986-2011). Initially
9747-516: Is the largest human chromosome with approximately 220 million base pairs , and would be 85 mm long if straightened. In eukaryotes , in addition to nuclear DNA , there is also mitochondrial DNA (mtDNA) which encodes certain proteins used by the mitochondria. The mtDNA is usually relatively small in comparison to the nuclear DNA. For example, the human mitochondrial DNA forms closed circular molecules, each of which contains 16,569 DNA base pairs, with each such molecule normally containing
9918-486: Is to allow the cell to replicate chromosome ends using the enzyme telomerase , as the enzymes that normally replicate DNA cannot copy the extreme 3′ ends of chromosomes. These specialized chromosome caps also help protect the DNA ends, and stop the DNA repair systems in the cell from treating them as damage to be corrected. In human cells , telomeres are usually lengths of single-stranded DNA containing several thousand repeats of
10089-657: The DNA sequence . Mutagens include oxidizing agents , alkylating agents and also high-energy electromagnetic radiation such as ultraviolet light and X-rays . The type of DNA damage produced depends on the type of mutagen. For example, UV light can damage DNA by producing thymine dimers , which are cross-links between pyrimidine bases. On the other hand, oxidants such as free radicals or hydrogen peroxide produce multiple forms of damage, including base modifications, particularly of guanosine, and double-strand breaks. A typical human cell contains about 150,000 bases that have suffered oxidative damage. Of these oxidative lesions,
10260-630: The MDU market, in which relationships are established with landlords, sometimes with contracts and exclusivity agreements for the buildings, sometimes to the anger of tenants. The rise of direct broadcast satellite systems providing the same type of programming using small satellite receivers, and of Verizon FiOS and other recent ventures by incumbent local exchange carriers such as U-verse , have also provided competition to incumbent cable television systems. Many cable channels charge cable providers "subscriber fees," in order to carry their content. The fee that
10431-499: The National Cable Television Association have recognized Walson as having invented cable television in the spring of 1948. A CATV system was developed in the late 1940s by James F. Reynolds in his town of Maple Dale, Pennsylvania, which grew to include Sandy Lake , Stoneboro , Polk , Cochranton , and Meadville . Even though Eastern Pennsylvania, particularly the counties of Schuylkill and Carbon in
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#173285122075610602-569: The TV Parental Guidelines , instead of the Motion Picture Association of America (MPAA) ratings system . Since the early 21st century, some have advocated for laws that would require cable providers to offer their subscribers their own " à la carte " choice of channels. Unlike the standardized subscription packages being offered currently, an à la carte model requires the customer to subscribe to each channel individually. It
10773-406: The amino-acid sequences of proteins is determined by the rules of translation , known collectively as the genetic code . The genetic code consists of three-letter 'words' called codons formed from a sequence of three nucleotides (e.g. ACT, CAG, TTT). In transcription, the codons of a gene are copied into messenger RNA by RNA polymerase . This RNA copy is then decoded by a ribosome that reads
10944-469: The cell nucleus as nuclear DNA , and some in the mitochondria as mitochondrial DNA or in chloroplasts as chloroplast DNA . In contrast, prokaryotes ( bacteria and archaea ) store their DNA only in the cytoplasm , in circular chromosomes . Within eukaryotic chromosomes, chromatin proteins, such as histones , compact and organize DNA. These compacting structures guide the interactions between DNA and other proteins, helping control which parts of
11115-432: The key demographic . That month, OWN also announced a planned expansion into scripted programming by means of an exclusive, multi-year development deal with Tyler Perry —a long-time friend of Winfrey. Perry's first two productions for OWN—the primetime soap opera The Haves and the Have Nots , and the sitcom Love Thy Neighbor —premiered on May 28 and 29, 2013 respectively. The two-hour series premiere of The Haves and
11286-637: The police murder of George Floyd . The special featured appearances by politicians such as Stacey Abrams and Mayor of Atlanta Keisha Lance Bottoms , and other journalists and personalities. The special was simulcast by all Discovery networks, and was seen by an average of 7.3 million viewers across both nights. In December 2020, Discovery increased its ownership in OWN from 73% to 95% for $ 35 million in an all-stock deal, giving Harpo approximately 1.34 million shares in Discovery. In April 2022, Discovery Inc. merged with WarnerMedia to form Warner Bros. Discovery ;
11457-419: The 3′ and 5′ carbons along the sugar-phosphate backbone confers directionality (sometimes called polarity) to each DNA strand. In a nucleic acid double helix , the direction of the nucleotides in one strand is opposite to their direction in the other strand: the strands are antiparallel . The asymmetric ends of DNA strands are said to have a directionality of five prime end (5′ ), and three prime end (3′), with
11628-588: The 5′ end having a terminal phosphate group and the 3′ end a terminal hydroxyl group. One major difference between DNA and RNA is the sugar, with the 2-deoxyribose in DNA being replaced by the related pentose sugar ribose in RNA. The DNA double helix is stabilized primarily by two forces: hydrogen bonds between nucleotides and base-stacking interactions among aromatic nucleobases. The four bases found in DNA are adenine ( A ), cytosine ( C ), guanine ( G ) and thymine ( T ). These four bases are attached to
11799-435: The DNA are transcribed. DNA is a long polymer made from repeating units called nucleotides . The structure of DNA is dynamic along its length, being capable of coiling into tight loops and other shapes. In all species it is composed of two helical chains, bound to each other by hydrogen bonds . Both chains are coiled around the same axis, and have the same pitch of 34 ångströms (3.4 nm ). The pair of chains have
11970-453: The DNA double helix that need to separate easily, such as the TATAAT Pribnow box in some promoters , tend to have a high AT content, making the strands easier to pull apart. In the laboratory, the strength of this interaction can be measured by finding the melting temperature T m necessary to break half of the hydrogen bonds. When all the base pairs in a DNA double helix melt,
12141-582: The FCC to exercise common carrier authority over 288 CATV systems in 36 states. The broadcasters maintained that CATV went against the FCC's Sixth Report and Order, which advocated at least one television station in every community. In 1958, the FCC decided that CATV was not really a common carrier since the subscriber did not determine the programming. Carter Mountain Transmission Corp., a common carrier that already transmitted television signals by microwave to CATV systems in several Wyoming towns, wanted to add
12312-446: The Have Nots overtook Life with La Toya (1.18 million) as OWN's highest-rated series premiere to date, with the first episode attracting 1.77 million viewers, and growing to 1.8 million for the second. The series continued to set viewership records for the channel, with its first-season finale drawing 2.6 million viewers, and the fifth episode of season 2 setting a network-record 3.6 million viewers. By July 2013, Discovery considered
12483-401: The RNA sequence by base-pairing the messenger RNA to transfer RNA , which carries amino acids. Since there are 4 bases in 3-letter combinations, there are 64 possible codons (4 combinations). These encode the twenty standard amino acids , giving most amino acids more than one possible codon. There are also three 'stop' or 'nonsense' codons signifying the end of the coding region; these are
12654-488: The TAG, TAA, and TGA codons, (UAG, UAA, and UGA on the mRNA). Cell division is essential for an organism to grow, but, when a cell divides, it must replicate the DNA in its genome so that the two daughter cells have the same genetic information as their parent. The double-stranded structure of DNA provides a simple mechanism for DNA replication . Here, the two strands are separated and then each strand's complementary DNA sequence
12825-678: The US peaked around the year 2000, at 68.5 million total subscriptions. Since then, cable subscriptions have been in slow decline, dropping to 54.4 million subscribers by December 2013. Some telephone service providers have started offering television, reaching to 11.3 million video subscribers as of December 2013. It is claimed that the first cable television system in the United States was created in 1948 in Mahanoy City, Pennsylvania by John Walson to provide television signals to people whose reception
12996-526: The United States in its first 24 years was used almost exclusively to relay terrestrial commercial television stations to remote and inaccessible areas. It also became popular in other areas in which mountainous terrain caused poor reception over the air. Original programming over cable came in 1972 with deregulation of the industry. During the Federal Communications Commission (FCC)'s freeze on television licenses from 1948 to 1952 ,
13167-479: The United States that would have determined the role of the FCC in CATV policy. Chief architect of some of these bills was attorney Yolanda G. Barco . She was one of the first female executives in cable, described as the "principal attorney for cable television interests during the industry's formative years". The 1959 bill, which made it to the floor of the Senate, would have limited FCC jurisdiction to CATV systems within
13338-484: The United States, and Tarlton himself became a highly sought-after consultant. Tarlton used equipment manufactured by a new company, Jerrold Electronics . After seeing the success of the Tarlton system in 1950, Jerrold president (and future Pennsylvania governor) Milton Shapp reorganized his company to build equipment for the now-growing cable industry. In 1952, Tarlton went to work for Jerrold, helping to construct most of
13509-405: The United States. After becoming Discovery Communications' new CEO in 2007, David Zaslav found Discovery Health to be underperforming along with its other digital cable networks launched in the last decade, and taking in significantly lower carriage fees in comparison to the company's namesake, Discovery Channel . As a result, he began to explore the possibility of re-launching the channel as
13680-438: The United States. While exclusive franchises are currently prohibited by federal law, and relatively few franchises were ever expressly exclusive, frequently only one cable company offers cable service in a given community. Overbuilders in the U.S., other than telephone companies with existing infrastructure, have traditionally had severe difficulty in financial and market penetration numbers. Overbuilders have had some success in
13851-432: The acquisition places OWN under common ownership with Warner Bros. Television —which has produced a number of OWN's recent scripted series. As of March 2014, the network's programming primarily consists of marathons , with eight-hour daytime blocks airing multiple episodes of the same series, usually a different show each day. The primetime programming varies and is repeated in late night, followed by an overnight movie and
14022-567: The air due to CATV competition at the time the government moved to expand its authority (nor have any since) did not stay the momentum for the expansion of regulatory authority. That some economic impact was merely plausible sufficed as the basis for government concern and government action". The FCC overruled a hearing examiner in favor of broadcasters again in the "San Diego Case". The CATV systems in San Diego, California wanted to import stations from Los Angeles, some of which could be seen in San Diego;
14193-451: The air in November 1948, Parsons was the only one in town able to see television. According to MSNBC 's Bob Sullivan, Parsons charged a $ 125 one-time set-up fee and a $ 3 a month service fee. In May 1968, Parsons was acknowledged as the father of community antenna television. In 1950, Robert Tarlton developed the first commercial cable television system in the United States. Tarlton organized
14364-414: The anthracite coal region, had several of the earliest CATV systems, there were other CATV entrepreneurs scattered throughout the United States. One was James Y. Davidson of Tuckerman, Arkansas . Davidson was the local movie theater manager and ran a radio repair business on the side. In 1949, he set up a cable system to bring the signal of a newly launched Memphis, Tennessee station to his community, which
14535-442: The axis of the double helix once every 10.4 base pairs, but if the DNA is twisted the strands become more tightly or more loosely wound. If the DNA is twisted in the direction of the helix, this is positive supercoiling, and the bases are held more tightly together. If they are twisted in the opposite direction, this is negative supercoiling, and the bases come apart more easily. In nature, most DNA has slight negative supercoiling that
14706-660: The cable industry for some time. Further, Smith's decision was influenced by his experiences testifying several times in United States Senate committee hearings. Senator, and future FCC commissioner, Kenneth A. Cox attended and participated in these hearings. He prepared a report for the Senate Committee on Interstate and Foreign Commerce against CATV and supporting the FCC policy of a television station in every community. In 1959 and 1961, bills were introduced in Congress of
14877-440: The cable provider offered "economy basic" subscriptions (local channels only; these appear at the lowest frequency signals, denoted by the lowest channel numbers) and "basic" subscriptions (local channels plus a handful of national channels with frequencies just higher than the local stations). Notch filters were used to filter out a "notch" of channels from an analog cable signal (for example, channels 45-50 could be "notched" out and
15048-465: The cable service provider must pay to a cable television channel can vary depending on whether it is a basic or premium channel and the perceived popularity of that channel. Because cable service providers are not required to carry all cable channels, they may negotiate the fee they will pay for carrying a channel. Typically, more popular cable channels command higher fees. For example, ESPN typically charges $ 10 per month for its suite of networks ($ 7 for
15219-420: The cable television system so that their signals can reach subscribers' homes. Additional cable television franchise fees and taxes are often tacked on by local, state, and federal governments. Most cable systems divide their channel lineups ("tiers") into three or four basic channel packages. A must-carry rule requires all cable television systems to carry all full-power local commercial broadcast stations in
15390-407: The canonical bases plus uracil. Twin helical strands form the DNA backbone. Another double helix may be found tracing the spaces, or grooves, between the strands. These voids are adjacent to the base pairs and may provide a binding site . As the strands are not symmetrically located with respect to each other, the grooves are unequally sized. The major groove is 22 ångströms (2.2 nm) wide, while
15561-467: The case of thalidomide, a teratogen . Others such as benzo[ a ]pyrene diol epoxide and aflatoxin form DNA adducts that induce errors in replication. Nevertheless, due to their ability to inhibit DNA transcription and replication, other similar toxins are also used in chemotherapy to inhibit rapidly growing cancer cells. DNA usually occurs as linear chromosomes in eukaryotes , and circular chromosomes in prokaryotes . The set of chromosomes in
15732-581: The cell (see below) , but the major and minor grooves are always named to reflect the differences in width that would be seen if the DNA was twisted back into the ordinary B form . In a DNA double helix, each type of nucleobase on one strand bonds with just one type of nucleobase on the other strand. This is called complementary base pairing . Purines form hydrogen bonds to pyrimidines, with adenine bonding only to thymine in two hydrogen bonds, and cytosine bonding only to guanine in three hydrogen bonds. This arrangement of two nucleotides binding together across
15903-588: The channel had as Discovery Health. On January 26, 2012, Lisa Erspamer resigned from OWN; she was replaced in the interim by veteran Discovery executive and former TLC vice president Rita Mullin. A March 2012 Oprah's Next Chapter interview with Whitney Houston 's daughter Bobbi Kristina Brown and her family (following Houston's death in February) became OWN's highest-rated single program to date, with 3.5 million viewers. The channel's losses were estimated to be $ 330 million as of May 2012. Discovery responded by raising
16074-412: The channel's retransmission consent fee (OWN was provided to cable providers effectively for free for its first two years on air) to a level more in line with other basic cable channels, a move that the company expected to allow the channel to break even in late-2013. On September 15, 2012, OWN premiered Iyanla: Fix My Life —a reality series starring Iyanla Vanzant . It attracted critical acclaim and
16245-619: The chromatin structure or else by remodeling carried out by chromatin remodeling complexes (see Chromatin remodeling ). There is, further, crosstalk between DNA methylation and histone modification, so they can coordinately affect chromatin and gene expression. For one example, cytosine methylation produces 5-methylcytosine , which is important for X-inactivation of chromosomes. The average level of methylation varies between organisms—the worm Caenorhabditis elegans lacks cytosine methylation, while vertebrates have higher levels, with up to 1% of their DNA containing 5-methylcytosine. Despite
16416-539: The co-owned W Network . Both Shaw Media and Corus share common ownership interests from the J.R. Shaw family, but are considered separate companies. Winfrey revealed to The New York Times that she is in final negotiations to release the channel in Brazil and Argentina . In April 2013, TLC UK began to air an OWN block . The same programming block began airing Discovery Home & Health in Australia on August 4, 2013. It
16587-516: The common practice of regularly paying to see films. The possibility of turning free television viewers into paid television viewers was discussed early on. For example, after 25 million American televisions tuned to a musical version of Cinderella in 1957, executives calculated that had the network received 25¢ for each television tuned to the show, it would have earned more than $ 6 million without distribution costs. However, due to many legal, regulatory and technological obstacles, cable television in
16758-476: The conditions found in cells, it is not a well-defined conformation but a family of related DNA conformations that occur at the high hydration levels present in cells. Their corresponding X-ray diffraction and scattering patterns are characteristic of molecular paracrystals with a significant degree of disorder. Compared to B-DNA, the A-DNA form is a wider right-handed spiral, with a shallow, wide minor groove and
16929-632: The contours (or the broadcast range) of a single station; however, the bill was defeated. The 1961 bill proposed by the FCC would have given the Commission authority over CATV as CATV, and not as a common carrier or broadcaster. The Commission could then adopt rules and regulations "in the public interest" to govern CATV in any area covered both by CATV and broadcast television. No action was ever taken on this bill. More important than Congressional action in determining Federal Communications Commission CATV policy were court cases and FCC hearings. In Frontier Broadcasting Co. v. Collier , broadcasters tried to compel
17100-405: The creation of new genes through the process of gene duplication and divergence . A gene is a sequence of DNA that contains genetic information and can influence the phenotype of an organism. Within a gene, the sequence of bases along a DNA strand defines a messenger RNA sequence, which then defines one or more protein sequences. The relationship between the nucleotide sequences of genes and
17271-455: The current state of television. OWN was not Winfrey's first cable television venture, having been co-founder of the women's cable network Oxygen . However, her involvement was limited to being an investor and eventually offering it an after-show component, Oprah After the Show , after deciding not to offer her reruns to the network; Winfrey distanced herself from Oxygen when discussing OWN, stressing
17442-685: The customer their choice of channels à la carte has become more cost-effective with the advent of digital cable, because a digital set-top converter box can be programmed remotely. IPTV (i.e., delivering TV channels over an internet or IP-based network) is even less labor-intensive, delivering channels to the consumer automatically. Currently, digital cable and satellite delivery systems with standardized subscriptions are providing an opportunity for networks that service niche and minority audiences to reach millions of households, and potentially, millions of viewers. Since à la carte could force each channel to be sold individually, such networks worry they could face
17613-449: The cytoplasm called the nucleoid . The genetic information in a genome is held within genes, and the complete set of this information in an organism is called its genotype . A gene is a unit of heredity and is a region of DNA that influences a particular characteristic in an organism. Genes contain an open reading frame that can be transcribed, and regulatory sequences such as promoters and enhancers , which control transcription of
17784-544: The date was later suspended. In 1972, Dean Burch steered the FCC into a new area of regulation. It lifted its restrictions on CATV in large cities, but now put the burden of more local programming on CATV operators. In 1976, the FCC used its rule-making power to require that new systems now had to have 20 channels, and that cable providers with systems of 3,500 subscribers or more had to provide Public, educational, and government access (PEG) services with facilities and equipment necessary to use this channel capacity . During
17955-402: The demand for television increased. Since new television station licenses were not being issued, the only way the demand was met, even in communities with one or more operating broadcast stations, was by Community Antenna Television (CATV) , as early cable was known (so named because of the literal sharing of a very large receiving antenna by an entire community). On August 1, 1949, T.J. Slowie,
18126-412: The designated television market on their lineups, unless those stations opt to invoke retransmission consent and demand compensation, in which case the cable provider can decline to carry the channel (especially if the provider feels that the rate of carrying an existing service would result in an increase of the average price of a tier to levels to which it could result in a subscriber possibly dropping
18297-405: The double helix (from six-carbon ring to six-carbon ring) is called a Watson-Crick base pair. DNA with high GC-content is more stable than DNA with low GC -content. A Hoogsteen base pair (hydrogen bonding the 6-carbon ring to the 5-carbon ring) is a rare variation of base-pairing. As hydrogen bonds are not covalent , they can be broken and rejoined relatively easily. The two strands of DNA in
18468-548: The early 1980s, various live local programs with local interests were rapidly being created all over the United States in most major television markets . Before there was public access TV , one of Time Inc.'s pioneering stations was in Columbus, Ohio , where Richard Sillman became the nation's youngest cable television director at age 16. Cable television programming is often divided between basic and premium television. Basic cable networks are generally those with wide carriage on
18639-442: The edges of the bases and chelation of a metal ion in the centre of each four-base unit. Other structures can also be formed, with the central set of four bases coming from either a single strand folded around the bases, or several different parallel strands, each contributing one base to the central structure. In addition to these stacked structures, telomeres also form large loop structures called telomere loops, or T-loops. Here,
18810-481: The end of an otherwise complementary double-strand of DNA. However, branched DNA can occur if a third strand of DNA is introduced and contains adjoining regions able to hybridize with the frayed regions of the pre-existing double-strand. Although the simplest example of branched DNA involves only three strands of DNA, complexes involving additional strands and multiple branches are also possible. Branched DNA can be used in nanotechnology to construct geometric shapes, see
18981-460: The end of her talk show, Winfrey's commitments with Discovery were revised; a commitment for Winfrey to host a talk show on OWN and appear in at least 35 hours of programming per year, was replaced with a commitment to appear in at least 70 hours of programming per year, including Oprah's Next Chapter —a primetime interview series hosted by Winfrey. The official launch of OWN was set for January 1, 2011, so that it would occur while Oprah's talk show
19152-474: The end of the year to pursue a job as head of content for the World Surf League . He will be succeeded by Tina Perry, who had worked business and legal affairs for MTV , VH1 , and OWN. On June 9 and 10, 2020, OWN broadcast OWN Spotlight: Where Do We Go From Here? — a two-night special hosted by Winfrey that discussed systemic racism and police brutality in U.S. society, in the wake of protests over
19323-421: The end of the year. In March 2011, the channel modified its schedule to only have three original nights of primetime programming per-week, moving Oprah Behind The Scenes to Sunday nights alongside Oprah's Master Class . On May 6, 2011, OWN's CEO Christina Norman was removed, and replaced by Discovery executive Peter Liguori as an interim CEO. In July, Winfrey assumed the role of CEO, and named Sheri Salata as
19494-625: The eponymous family's predominantly-African American megachurch in Memphis, Tennessee . In July 2017, Queen Sugar was renewed for a third season, and Harpo Films signed DuVernay to a first-look television and digital media deal. In August 2017, OWN also ordered Tarell Alvin McCraney 's David Makes Man , a coming-of-age drama set in South Florida . McCraney stated that he was given offers by other outlets such as Netflix , but that Winfrey (who had made
19665-561: The fifth and fourth seasons of The Haves and the Have Nots and If Loving You Is Wrong . On February 2, 2015, OWN announced that it had ordered Ava DuVernay 's drama Queen Sugar (based on the Natalie Baszile novel of the same name ) directly to series, marking OWN's first scripted series not produced by Tyler Perry. This was followed in July by Greenleaf —a new drama from Craig Wright with Winfrey as an executive producer, that follows
19836-530: The film deal began immediately, while the television/short form deal will begin after the end of his pact with OWN in May 2019. OWN stated that Perry's series would continue to air on the network through at least 2020, and that it would "continue to aggressively expand its roster of premium original scripted series." In December 2017, Discovery announced that it would acquire an additional 24.5% stake in OWN that it did not already own, for $ 70 million, with Harpo remaining as
20007-661: The first adult-oriented premium cable services–have even offered softcore pornography as part of their programming inventory. While there are no FCC rules that apply to content on basic cable networks, many self-regulate their program content due to demographic targeting, or because of viewer and advertiser expectations, particularly with regard to profane language and nudity. In recent years, however, some networks have become more lenient towards content aired during late-primetime and late-night hours. In addition, some channels, such as FX , have positioned themselves with an original programming direction more akin to premium services, with
20178-633: The first cable network to be delivered nationwide by satellite transmission. Although such conversions are rare, some present-day basic cable channels have originated as premium services, including the Disney Channel (from 1983 to 1997), AMC (from 1984 to 1988), and Bravo (from 1982 to 1994); some of these services eventually switched to an advertiser-supported model after transitioning to an unencrypted structure. Other fledgling premium services (such as early HBO spin-off efforts Take 2 and Festival , Home Theater Network and Spotlight ) have lasted for
20349-418: The focus is on the interactions between DNA and other molecules that mediate the function of the genome. Genomic DNA is tightly and orderly packed in the process called DNA condensation , to fit the small available volumes of the cell. In eukaryotes, DNA is located in the cell nucleus , with small amounts in mitochondria and chloroplasts . In prokaryotes, the DNA is held within an irregularly shaped body in
20520-402: The former Canadian version of Discovery Health, owned by Shaw Media , did not become a Canadian version of OWN, instead shifting to a reality-based format known as Twist TV . Instead, Corus Entertainment agreed to launch a Canadian version of OWN—its existing channel Viva was re-launched as OWN on March 1, 2011. During January and February 2011, selected OWN programming aired on both Viva and
20691-461: The four major types of macromolecules that are essential for all known forms of life . The two DNA strands are known as polynucleotides as they are composed of simpler monomeric units called nucleotides . Each nucleotide is composed of one of four nitrogen-containing nucleobases ( cytosine [C], guanine [G], adenine [A] or thymine [T]), a sugar called deoxyribose , and a phosphate group . The nucleotides are joined to one another in
20862-448: The functions of these RNAs are not entirely clear. One proposal is that antisense RNAs are involved in regulating gene expression through RNA-RNA base pairing. A few DNA sequences in prokaryotes and eukaryotes, and more in plasmids and viruses , blur the distinction between sense and antisense strands by having overlapping genes . In these cases, some DNA sequences do double duty, encoding one protein when read along one strand, and
21033-448: The helical axis in a left-handed spiral, the opposite of the more common B form. These unusual structures can be recognized by specific Z-DNA binding proteins and may be involved in the regulation of transcription. For many years, exobiologists have proposed the existence of a shadow biosphere , a postulated microbial biosphere of Earth that uses radically different biochemical and molecular processes than currently known life. One of
21204-448: The hydration level, DNA sequence, the amount and direction of supercoiling, chemical modifications of the bases, the type and concentration of metal ions , and the presence of polyamines in solution. The first published reports of A-DNA X-ray diffraction patterns —and also B-DNA—used analyses based on Patterson functions that provided only a limited amount of structural information for oriented fibers of DNA. An alternative analysis
21375-427: The hydrolytic activities of cellular water, etc., also occur frequently. Although most of these damages are repaired, in any cell some DNA damage may remain despite the action of repair processes. These remaining DNA damages accumulate with age in mammalian postmitotic tissues. This accumulation appears to be an important underlying cause of aging. Many mutagens fit into the space between two adjacent base pairs, this
21546-412: The importance of 5-methylcytosine, it can deaminate to leave a thymine base, so methylated cytosines are particularly prone to mutations . Other base modifications include adenine methylation in bacteria, the presence of 5-hydroxymethylcytosine in the brain , and the glycosylation of uracil to produce the "J-base" in kinetoplastids . DNA can be damaged by many sorts of mutagens , which change
21717-409: The importance of actually having influence over programming when participating in such ventures. In November 2008, Zaslav stated that Winfrey had planned to not renew her contract with CBS for The Oprah Winfrey Show beyond the 2010–11 season, and that the show could move to OWN in some form following the end of the syndicated run. Harpo Productions denied the report, stating that Winfrey "has not made
21888-472: The importation of distant signals into the top 100 markets, thus making CATV at that time profitable only in cities with poor reception. In 1968, the Supreme Court upheld the FCC's right to make rules and regulations concerning CATV. In its decision on United States v. Southwestern Cable , the "San Diego Case", it said "the Commission's authority over 'all interstate ... communications by wire or radio' permits
22059-463: The launch of OWN, Discovery Health's programming was merged into another Discovery-owned channel, FitTV—which rebranded as Discovery Fit & Health a month later on February 1, 2011. It was subsequently re-branded again as Discovery Life in 2015. During its first year of operation, OWN struggled to build an audience and viewership, with most of its programs being low-rated besides Our America with Lisa Ling (the first OWN program to be renewed for
22230-404: The lowest service tiers of multichannel television providers. In the era of analog cable television, these channels were typically transmitted without any encryption or other scrambling methods. These networks can vary in format, ranging from those targeting mainstream audiences, to specialty networks that are focused on specific genres , demographics , or niches. Basic cable networks depend on
22401-519: The main channel alone), by far the highest of any non-premium American cable channel, comparable to the premium channels, and rising rapidly. Other widely viewed cable channels have been able to command fees of over 50 cents per subscriber per month; channels can vary widely in fees depending on if they are included in package deals with other channels. DNA Deoxyribonucleic acid ( / d iː ˈ ɒ k s ɪ ˌ r aɪ b oʊ nj uː ˌ k l iː ɪ k , - ˌ k l eɪ -/ ; DNA )
22572-568: The major systems built by that company in the 1950s. Tarlton was also responsible for training many of the major operators of cable systems in the 1950s. In 2003, Tarlton was inducted in the Cable Television Hall of Fame for his work building the first widely publicized cable television company in America. The rise of free broadcast television during the 1950s greatly threatened the established entertainment industry by offering an alternative to
22743-441: The minor groove is 12 Å (1.2 nm) in width. Due to the larger width of the major groove, the edges of the bases are more accessible in the major groove than in the minor groove. As a result, proteins such as transcription factors that can bind to specific sequences in double-stranded DNA usually make contact with the sides of the bases exposed in the major groove. This situation varies in unusual conformations of DNA within
22914-516: The mitochondrial genome (constituting up to 90% of the DNA of the cell). A DNA sequence is called a "sense" sequence if it is the same as that of a messenger RNA copy that is translated into protein. The sequence on the opposite strand is called the "antisense" sequence. Both sense and antisense sequences can exist on different parts of the same strand of DNA (i.e. both strands can contain both sense and antisense sequences). In both prokaryotes and eukaryotes, antisense RNA sequences are produced, but
23085-477: The most dangerous are double-strand breaks, as these are difficult to repair and can produce point mutations , insertions , deletions from the DNA sequence, and chromosomal translocations . These mutations can cause cancer . Because of inherent limits in the DNA repair mechanisms, if humans lived long enough, they would all eventually develop cancer. DNA damages that are naturally occurring , due to normal cellular processes that produce reactive oxygen species,
23256-434: The network to be "cash flow positive". In an interview with People , Winfrey revealed that in 2012, she had a nervous breakdown over the channel's performance. Jason Russell had brought up the condition during an interview they were filming, which made Winfrey realize she was experiencing similar symptoms. She explained that "after 25 years of being number one, I had become accustomed to success. I didn't expect failure. I
23427-499: The network. The June 2016 premiere of Greenleaf was OWN's highest-rated series premiere to date, with 3.04 million viewers. Queen Sugar also premiered later in September to acclaim, with both series ranking among the top 5 ad-supported drama series on cable among women 25–54. In July 2017, Tyler Perry signed a new film and television/short form development deal with Viacom for future productions (particularly on BET ) through 2024;
23598-464: The new strand, and the cell ends up with a perfect copy of its DNA. Naked extracellular DNA (eDNA), most of it released by cell death, is nearly ubiquitous in the environment. Its concentration in soil may be as high as 2 μg/L, and its concentration in natural aquatic environments may be as high at 88 μg/L. Various possible functions have been proposed for eDNA: it may be involved in horizontal gene transfer ; it may provide nutrients; and it may act as
23769-454: The open reading frame. In many species , only a small fraction of the total sequence of the genome encodes protein. For example, only about 1.5% of the human genome consists of protein-coding exons , with over 50% of human DNA consisting of non-coding repetitive sequences . The reasons for the presence of so much noncoding DNA in eukaryotic genomes and the extraordinary differences in genome size , or C-value , among species, represent
23940-792: The pay-television industry that operated during the 1950s and 1960s (with a few systems lingering until 1980), as well as some attempts by free-to-air broadcasters during the 1970s and 1980s that ultimately folded as their subscriber bases declined amid viewer shifts to receiving premium television content delivered by cable providers that had begun operating in metropolitan areas throughout that period. In its infancy, following its launch over Service Electric Cable 's Wilkes-Barre, Pennsylvania , system on November 8, 1972, HBO had been quietly providing pay programming to CATV systems in Pennsylvania and New York , using microwave technology to transmit its programming to cable and MMDS providers. In 1975, HBO became
24111-428: The place of thymine in RNA and differs from thymine by lacking a methyl group on its ring. In addition to RNA and DNA, many artificial nucleic acid analogues have been created to study the properties of nucleic acids, or for use in biotechnology. Modified bases occur in DNA. The first of these recognized was 5-methylcytosine , which was found in the genome of Mycobacterium tuberculosis in 1925. The reason for
24282-458: The power to regulate CATV. This Report and Order was designed to protect television stations in small towns. It did this by imposing two rules, which slightly altered form: one requires that a CATV system carry all local stations in which the CATV system is in the A- (best reception) contour of the station. The second prohibits the importation of programs from a non-local station that duplicates programming on
24453-663: The premiere of a new Sunday program, Super Soul Sunday , which would include features and segments that "present an array of perspectives on what it means to be alive in today's world." The Rosie Show —a talk show hosted by another formerly-syndicated personality, Rosie O'Donnell —also premiered in October, to an average of 497,000 viewers (along with an additional million via a simulcast across sister channels Discovery Fit & Health, Investigation Discovery , Planet Green , and TLC ). By November, its ratings had fallen to an average of 171,000 nightly viewers. In January 2012, an attempt
24624-530: The presence of these noncanonical bases in bacterial viruses ( bacteriophages ) is to avoid the restriction enzymes present in bacteria. This enzyme system acts at least in part as a molecular immune system protecting bacteria from infection by viruses. Modifications of the bases cytosine and adenine, the more common and modified DNA bases, play vital roles in the epigenetic control of gene expression in plants and animals. A number of noncanonical bases are known to occur in DNA. Most of these are modifications of
24795-591: The price of basic cable can be regulated by local authorities as part of their franchise agreements . Standard, or expanded basic, cable is not subject to price controls. In addition to the basic cable packages, all systems offer premium channel add-on packages offering either just one premium network (for example, HBO) or several premium networks for one price (for example, HBO and Showtime together). Finally, most cable systems offer pay-per-view channels where users can watch individual movies, live events, sports and other programs for an additional fee for single viewing at
24966-412: The prime symbol being used to distinguish these carbon atoms from those of the base to which the deoxyribose forms a glycosidic bond . Therefore, any DNA strand normally has one end at which there is a phosphate group attached to the 5′ carbon of a ribose (the 5′ phosphoryl) and another end at which there is a free hydroxyl group attached to the 3′ carbon of a ribose (the 3′ hydroxyl). The orientation of
25137-466: The proposals was the existence of lifeforms that use arsenic instead of phosphorus in DNA . A report in 2010 of the possibility in the bacterium GFAJ-1 was announced, though the research was disputed, and evidence suggests the bacterium actively prevents the incorporation of arsenic into the DNA backbone and other biomolecules. At the ends of the linear chromosomes are specialized regions of DNA called telomeres . The main function of these regions
25308-543: The regulation of CATV systems." Carriage refers to the agreement under which a cable provider rebroadcasts a television channel on its network. The Federal Communications Commission puts various requirements on these agreements, which may include channels cable providers are required to carry, and moderates disputes over the fees and conditions of any particular agreement. In 1969, the FCC issued rules requiring all CATV systems with over 3,500 subscribers to have facilities for local origination of programming by April 1, 1971;
25479-407: The same available bandwidth , by converting cable channels to a digital signal and then compressing the signal. Currently, most systems offer a hybrid analog/digital cable system. This means they offer a certain number of analog channels via their basic cable service with additional channels being made available via their digital cable service. Digital cable channels are touted as being able to offer
25650-401: The second half of 2009. Winfrey would serve as the chairwoman of the channel, which was expected to deal in factual programming oriented towards her personal philosophy of "living your best life"; this would include topics such as health, love, parenting, and spirituality. Discovery provided $ 100 million in funding, and Harpo provided access to its library and Winfrey's website, Oprah.com. As it
25821-432: The section on uses in technology below. Several artificial nucleobases have been synthesized, and successfully incorporated in the eight-base DNA analogue named Hachimoji DNA . Dubbed S, B, P, and Z, these artificial bases are capable of bonding with each other in a predictable way (S–B and P–Z), maintain the double helix structure of DNA, and be transcribed to RNA. Their existence could be seen as an indication that there
25992-431: The sequence of amino acids within proteins in a process called translation . Within eukaryotic cells, DNA is organized into long structures called chromosomes . Before typical cell division , these chromosomes are duplicated in the process of DNA replication, providing a complete set of chromosomes for each daughter cell. Eukaryotic organisms ( animals , plants , fungi and protists ) store most of their DNA inside
26163-762: The service). Cable television systems are also required to offer a subscription package that provides these broadcast channels at a lower rate than the standard subscription rate. The basic programming package offered by cable television systems is usually known as "basic cable" and provides access to a large number of cable television channels, as well as broadcast television networks (e.g., ABC , CBS , NBC , Fox , The CW , MyNetworkTV , Telemundo , Univision , UniMás , PBS ), public, educational, and government access channels, free or low-cost public service channels such as C-SPAN and NASA TV , and several channels devoted to infomercials , brokered televangelism and home shopping to defray costs. Some providers may provide
26334-473: The shape of a double helix . The nucleotide contains both a segment of the backbone of the molecule (which holds the chain together) and a nucleobase (which interacts with the other DNA strand in the helix). A nucleobase linked to a sugar is called a nucleoside , and a base linked to a sugar and to one or more phosphate groups is called a nucleotide . A biopolymer comprising multiple linked nucleotides (as in DNA)
26505-552: The single-ringed pyrimidines and the double-ringed purines . In DNA, the pyrimidines are thymine and cytosine; the purines are adenine and guanine. Both strands of double-stranded DNA store the same biological information . This information is replicated when the two strands separate. A large part of DNA (more than 98% for humans) is non-coding , meaning that these sections do not serve as patterns for protein sequences . The two strands of DNA run in opposite directions to each other and are thus antiparallel . Attached to each sugar
26676-498: The single-stranded DNA curls around in a long circle stabilized by telomere-binding proteins. At the very end of the T-loop, the single-stranded telomere DNA is held onto a region of double-stranded DNA by the telomere strand disrupting the double-helical DNA and base pairing to one of the two strands. This triple-stranded structure is called a displacement loop or D-loop . In DNA, fraying occurs when non-complementary regions exist at
26847-518: The strands separate and exist in solution as two entirely independent molecules. These single-stranded DNA molecules have no single common shape, but some conformations are more stable than others. In humans, the total female diploid nuclear genome per cell extends for 6.37 Gigabase pairs (Gbp), is 208.23 cm long and weighs 6.51 picograms (pg). Male values are 6.27 Gbp, 205.00 cm, 6.41 pg. Each DNA polymer can contain hundreds of millions of nucleotides, such as in chromosome 1 . Chromosome 1
27018-647: The subscriber still receives channels below 45 and above 50). This allowed cable providers to open standardized ranges of premium channels to the subscriber, but notch filtering was not a feasible way to offer each subscriber their own individual choice of channels. To offer "à la carte" service using an analog signal, a cable provider would most likely have to scramble every channel and send a technician to each subscriber's home to unscramble their choice of channels on their set-top box. Each change an analog cable customer made in their subscription would then require an additional home visit to reprogram their set-top box. Offering
27189-469: The sugar-phosphate to form the complete nucleotide, as shown for adenosine monophosphate . Adenine pairs with thymine and guanine pairs with cytosine, forming A-T and G-C base pairs . The nucleobases are classified into two types: the purines , A and G , which are fused five- and six-membered heterocyclic compounds , and the pyrimidines , the six-membered rings C and T . A fifth pyrimidine nucleobase, uracil ( U ), usually takes
27360-417: The television stations in San Diego did not want the signals to be imported. The television stations won, not allowing the signals on future cable lines in San Diego and its environs. The FCC's reasoning was to protect existing and future UHF stations in San Diego. (One of the pioneers of cable TV was KSA-TV ) In the First Report and Order by the Federal Communications Commission on CATV, the FCC gave itself
27531-408: The use of a converter box ). Because their programming is commercial-free (except for promotions in-between shows for the networks' own content), these networks command much higher fees from cable systems. Premium services have the discretion to offer the service unencrypted to a certain number of participating cable providers during a short-term free preview period to allow those who do not receive
27702-666: Was announced on September 20, 2013, that the OWN Programming Block would begin airing in South Africa on DStv beginning October 17, 2013. OWN Programming Block also debuted in Poland and Russia in August and September; it also debuted on October 28, 2013, in Balkans/Romania/Bulgaria. Basic cable According to reports released by the Federal Communications Commission , traditional cable television subscriptions in
27873-516: Was located too far away to receive the signal with set-top antennas alone. Leroy E. "Ed" Parsons built the first cable television system in the United States that used coaxial cable , amplifiers, and a community antenna to deliver television signals to an area that otherwise would not have been able to receive broadcast television signals. In 1948, Parsons owned a radio station in Astoria, Oregon . A year earlier he and his wife had first seen television at
28044-452: Was made to retool the program by hiring Shane Farley (producer of the syndicated Rosie O'Donnell Show ), and giving it a smaller, more intimate studio. However, viewership was still low, and the series was cancelled in March 2012. During its first year of operations, OWN's average primetime audience was 264,000 viewers. This was only slightly higher than the average primetime audience of 216,000
28215-468: Was poor because of tall mountains and buildings blocking TV signals. Mahanoy City was ideally suited for CATV services, since broadcast television signals could easily be received via mountaintop antennas and retransmitted by "twin-lead" or "ladder-lead" cable to the valley community below (where broadcast reception was very poor). Walson's "first" claim has long been questioned and his claimed starting date can not be verified. The United States Congress and
28386-517: Was proposed by Wilkins et al. in 1953 for the in vivo B-DNA X-ray diffraction-scattering patterns of highly hydrated DNA fibers in terms of squares of Bessel functions . In the same journal, James Watson and Francis Crick presented their molecular modeling analysis of the DNA X-ray diffraction patterns to suggest that the structure was a double helix. Although the B-DNA form is most common under
28557-433: Was still on the air. Plans now called for at least 600 hours of original programming in 2011, as well as acquired and library programming (including reruns of The Oprah Winfrey Show later in the year, and Dr. Phil ); although Winfrey did make appearances in some of the programming aired on-launch (such as Oprah's Master Class ), her involvement was expected to increase following the end of The Oprah Winfrey Show . After
28728-593: Was still under contract with CBS Television Distribution through May 2011, her existing syndicated talk show The Oprah Winfrey Show was not expected to air on OWN, but there was a possibility that a spiritual continuation or successor to the program could air on OWN in the future. Winfrey and her long-time partner Stedman Graham had discussed the idea of forming her own channel as early as 1992. After Winfrey demonstrated discontent over "confrontational" talk shows on television, Graham suggested that she should form an "Oprah Winfrey Network" if she were dissatisfied with
28899-829: Was subsequently renamed Fox Family in 1998 after it was acquired by a partnership between Fox Entertainment Group and Saban Entertainment , then ABC Family after its 2001 sale to ABC parent The Walt Disney Company , and finally to its current name, Freeform in 2016. The origins of premium cable lie in two areas: early pay television systems of the 1950s and 1960s and early cable (CATV) operators' small efforts to add extra channels to their systems that were not derived from free-to-air signals. In more recent years, premium cable refers to networks–such as Home Box Office (HBO) , Cinemax , Showtime , The Movie Channel , Flix , Starz , MoviePlex , and Epix –that scramble or encrypt their signals so that only those paying additional monthly fees to their cable system can legally view them (via
29070-409: Was tested and I had to dig deep", and that the scenario "forced me not to just talk the talk. Failure is a great teacher. I knew this intellectually. But it's another thing if you're living it.". In May 2016, Sheri Salata stepped down as co-president to launch a new "brand innovation" agency (although Winfrey and OWN were expected to be among her first clients). This left Erik Logan as sole president of
29241-736: Was the CBN Satellite Service, a Christian television service launched by televangelist Pat Robertson in April 1977 as the television ministry of his Christian Broadcasting Network , that was delivered by satellite as a more efficient way to distribute the programming. For years, the CBN Satellite Service (later renamed CBN Cable Network in 1984) mixed religious programming with reruns of classic television series to fill out its 24-hour schedule. The network changed its name to The CBN Family Channel in 1988 (revised to The Family Channel in 1990 once CBN spun it out to an indirectly owned for-profit company, International Family Entertainment ). It
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