41-445: Internalins are surface proteins found on Listeria monocytogenes . They exist in two known forms, InlA and InlB. They are used by the bacteria to invade mammalian cells via cadherins transmembrane proteins and Met receptors respectively. The exact role of these proteins and their invasiveness in vivo is not completely understood. In cultured cells, InlA is necessary to facilitate Listeria entry into human epithelial cells, while InlB
82-455: A bacillus, L. monocytogenes septates in the middle of the cell, thus has one new pole and one old pole) are capable of binding the Arp2/3 complex , thereby inducing actin nucleation at a specific area of the bacterial cell surface. Actin polymerization then propels the bacterium unidirectionally into the host cell membrane. The protrusion formed may then be internalized by a neighboring cell, forming
123-485: A bacterial protein internalin (InlA/InlB), which attaches to a protein on the intestinal cell membrane "cadherin" and allows the bacteria to invade the cells through a zipper mechanism. These adhesion molecules are also to be found in two other unusually tough barriers in humans — the blood-brain barrier and the fetal–placental barrier, and this may explain the apparent affinity that L. monocytogenes has for causing meningitis and affecting babies in utero . Once inside
164-411: A beta hemolysin , which causes destruction of red blood cells. This bacterium exhibits characteristic tumbling motility when viewed with light microscopy. Although L. monocytogenes is actively motile by means of peritrichous flagella at room temperature (20−25 °C), the organism does not synthesize flagella at body temperatures (37 °C). The genus Listeria belongs to the class Bacilli and
205-491: A double-membrane vacuole from which the bacterium must escape using LLO and PlcB. This mode of direct cell-to-cell spread involves a cellular mechanism known as paracytophagy . The ability of L. monocytogenes to successfully infect depends on its resistance to the high concentrations of bile encountered throughout the gastrointestinal tract. This resistance is due, in part, to the nucleotide excision repair protein UvrA that
246-494: A glycolipid moiety, such as a galactosyl-glucosyl-diglyceride, is covalently linked to the terminal phosphomonoester of the teichoic acid. This lipid region anchors the polymer chain to the cytoplasmic membrane. These lipoteichoic acids resemble the lipopolysaccharides of Gram-negative bacteria in both structure and function, being the only amphipathic polymers at the cell surface. L. monocytogenes has D-galactose residues on its surface that can attach to D-galactose receptors on
287-458: A variety of other tests. Total time to identification takes five to seven days, but the announcement of specific non-radiolabelled DNA probes should soon allow a simpler and faster confirmation of suspect isolates. Recombinant DNA technology may even permit two- to three-day positive analysis in the future. Currently, the FDA is collaborating in adapting its methodology to quantitate very low numbers of
328-430: A vector to deliver genes in vitro . Current transfection efficiency remains poor. One example of the successful use of L. monocytogenes in in vitro transfer technologies is in the delivery of gene therapies for cystic fibrosis cases. Listeria monocytogenes is being investigated as a cancer immunotherapy for several types of cancer. A live attenuated Listeria monocytogenes cancer vaccine , ADXS11-001 ,
369-520: Is a Gram-positive bacterium , in the phylum Bacillota . Its ability to grow at temperatures as low as 0 °C permits multiplication at typical refrigeration temperatures, greatly increasing its ability to evade control in human foodstuffs. Motile via flagella at 30 °C and below, but usually not at 37 °C, L. monocytogenes can instead move within eukaryotic cells by explosive polymerization of actin filaments (known as comet tails or actin rockets). Once Listeria monocytogenes enters
410-509: Is a stub . You can help Misplaced Pages by expanding it . Listeria monocytogenes Listeria monocytogenes is the species of pathogenic bacteria that causes the infection listeriosis . It is a facultative anaerobic bacterium, capable of surviving in the presence or absence of oxygen. It can grow and reproduce inside the host's cells and is one of the most virulent foodborne pathogens. Twenty to thirty percent of foodborne listeriosis infections in high-risk individuals may be fatal. In
451-431: Is necessary for Listeria internalisation in several other cell types, including hepatocytes , fibroblasts , and epithelioid cells. Internalins are mainly surface-exposed virulence factors present in a number of Gram-positive bacteria whose role ranges from recognition of cellular receptors to aid in pathogen entry to escape from autophagy. Listeria poses a particular threat to pregnant women because of its ability to cross
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#1732859243875492-700: Is now widely recognized as an important hazard in the food industry. Invasive infection by L. monocytogenes causes the disease listeriosis. When the infection is not invasive, any illness as a consequence of infection is termed febrile gastroenteritis. The manifestations of listeriosis include sepsis, meningitis (or meningoencephalitis ), encephalitis , corneal ulcer, pneumonia, myocarditis, and intrauterine or cervical infections in pregnant women, which may result in spontaneous abortion (second to third trimester) or stillbirth . Surviving neonates of fetomaternal listeriosis may suffer granulomatosis infantiseptica — pyogenic granulomas distributed over
533-655: Is quite hardy and resists the deleterious effects of freezing, drying, and heat remarkably well for a bacterium that does not form spores. Most Listeria monocytogenes strains are pathogenic to some degree. Listeria monocytogenes has been associated with such foods as raw milk , pasteurized fluid milk, cheeses (particularly soft-ripened varieties), hard-boiled eggs, ice cream , raw vegetables , fermented raw-meat sausages , raw and cooked poultry , raw meats (of all types), and raw and smoked fish . Most bacteria can survive near freezing temperatures, but cannot absorb nutrients, grow or replicate; however, L. monocytogenes has
574-424: Is the third most common cause of meningitis in newborns. Listeria monocytogenes can infect the brain, spinal-cord membranes and bloodstream of the host through the ingestion of contaminated food such as unpasteurized dairy or raw foods. L. monocytogenes is a Gram-positive , non- spore-forming , motile, facultatively anaerobic, rod-shaped bacterium. It is catalase -positive and oxidase -negative, and expresses
615-400: Is to exploit the junction-forming apparatus of the host into internalizing the bacterium. L. monocytogenes can also invade phagocytic cells (e.g., macrophages ), but requires only internalins for invasion of nonphagocytic cells. Following internalization, the bacterium must escape from the vacuole/ phagosome before fusion with a lysosome can occur. Three main virulence factors that allow
656-417: Is under development as a possible treatment for cervical carcinoma . Researchers have found Listeria monocytogenes in at least 37 mammalian species , both domesticated and feral, as well as in at least 17 species of birds and possibly in some species of fish and shellfish . Laboratories can isolate Listeria monocytogenes from soil , silage , and other environmental sources. Listeria monocytogenes
697-431: Is unknown, but may range from a few days to 3 weeks. The onset time to gastrointestinal symptoms is unknown, but probably exceeds 12 hours. An early study suggested that L. monocytogenes is unique among Gram-positive bacteria in that it might possess lipopolysaccharide , which serves as an endotoxin . Later, it was found to not be a true endotoxin. Listeria cell walls consistently contain lipoteichoic acids , in which
738-463: Is used in the identification of L. monocytogenes ; instillation of a culture into the conjunctival sac of a rabbit or guinea pig causes severe keratoconjunctivitis within 24 hours. Listeria species grow on media such as Mueller-Hinton agar. Identification is enhanced if the primary cultures are done on agar containing sheep blood, because the characteristic small zone of hemolysis can be observed around and under colonies. Isolation can be enhanced if
779-742: The European Union , listeriosis continues an upward trend that began in 2008, causing 2,161 confirmed cases and 210 reported deaths in 2014, 16% more than in 2013. In the EU, listeriosis mortality rates also are higher than those of other foodborne pathogens. Responsible for an estimated 1,600 illnesses and 260 deaths in the United States annually, listeriosis ranks third in total number of deaths among foodborne bacterial pathogens, with fatality rates exceeding even Salmonella spp. and Clostridium botulinum . Named for Joseph Lister , Listeria monocytogenes
820-459: The 1920s, it was not recognized as a significant cause of neonatal infection , sepsis , and meningitis until 1952 in East Germany . Listeriosis in adults was later associated with patients living with compromised immune systems, such as individuals taking immunosuppressant drugs and corticosteroids for malignancies or organ transplants, and those with HIV infection. L. monocytogenes
861-457: The 1980s. L. monocytogenes was first described by E.G.D. Murray (Everitt George Dunne Murray) in 1924 based on six cases of sudden death in young rabbits, and published a description with his colleagues in 1926 . Murray referred to the organism as Bacterium monocytogenes before Harvey Pirie changed the genus name to Listeria in 1940. Although clinical descriptions of L. monocytogenes infection in both animals and humans were published in
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#1732859243875902-400: The ability to grow at temperatures as low as 0 °C which permits exponential multiplication in refrigerated foods. At refrigeration temperature, such as 4 °C, the amount of ferric iron can affect the growth of L. monocytogenes . The primary site of infection is the intestinal epithelium, where the bacteria invade nonphagocytic cells via the "zipper" mechanism. Uptake is stimulated by
943-429: The bacteria to use the host cell's actin polymerization machinery to polymerize the cytoskeleton to give a "boost" to the bacterial cell so it can move in the cell. The same mechanism also allows the bacteria to travel from cell to cell. L. monocytogenes can act as a saprophyte or a pathogen , depending on its environment. When this bacterium is present within a host organism, quorum sensing and other signals cause
984-431: The bacterium to escape are listeriolysin O (LLO encoded by hly ) phospholipase A (encoded by plcA ) and phospholipase B ( plcB ). Secretion of LLO and PlcA disrupts the vacuolar membrane and allows the bacterium to escape into the cytoplasm, where it may proliferate. Once in the cytoplasm, L. monocytogenes exploits host actin for the second time. ActA proteins associated with the old bacterial cell pole (being
1025-416: The bacterium. The U.S. Food and Drug Administration (FDA) approved a cocktail of six bacteriophages from Intralytix, and a one-type phage product from EBI Food Safety designed to kill L. monocytogenes . Uses would potentially include spraying it on fruits and ready-to-eat meat such as sliced ham and turkey. Because L. monocytogenes is an intracellular bacterium, some studies have used this bacterium as
1066-592: The binding of listerial internalins (Inl) to E-cadherin , a host cell adhesion factor, or Met ( c-Met ), hepatocyte growth factor. This binding activates certain Rho-GTPases, which subsequently bind and stabilize Wiskott–Aldrich syndrome protein (WASp). WASp can then bind the Arp2/3 complex and serve as an actin nucleation point. Subsequent actin polymerization creates a "phagocytic cup", an actin-based structure normally formed around foreign materials by phagocytes prior to endocytosis. The net effect of internalin binding
1107-578: The bloodstream. The infectious dose of L. monocytogenes varies with the strain and with the susceptibility of the victim. From cases contracted through raw or supposedly pasteurized milk, one may safely assume that, in susceptible persons, fewer than 1,000 total organisms may cause disease. L. monocytogenes may invade the gastrointestinal epithelium. Once the bacterium enters the host's monocytes , macrophages , or polymorphonuclear leukocytes , it becomes bloodborne (sepsis) and can grow. Its presence intracellularly in phagocytic cells also permits access to
1148-419: The brain and probably transplacental migration to the fetus in pregnant women. This process is known as the "Trojan Horse mechanism". The pathogenesis of L. monocytogenes centers on its ability to survive and multiply in phagocytic host cells. It seems that Listeria originally evolved to invade membranes of the intestines, as an intracellular infection, and developed a chemical mechanism to do so. This involves
1189-424: The cell, L. monocytogenes rapidly acidifies the lumen of the vacuole formed around it during cell entry to activate listeriolysin O, a cholesterol-dependent cytolysin capable of disrupting the vacuolar membrane. This frees the pathogen and gives it access to the cytosol of the cell, where it continues its pathogenesis. Motility in the intracellular space is provided by actin assembly-inducing protein, which allows
1230-586: The genus Listeria , was reclassified into the new genus Jonesia . Both L. ivanovii and L. monocytogenes are pathogenic in mice, but only L. monocytogenes is consistently associated with human illness. The 13 serotypes of L. monocytogenes can cause disease, but more than 90% of human isolates belong to only three serotypes: 1/2a, 1/2b, and 4b. L. monocytogenes serotype 4b strains are responsible for 33 to 35% of sporadic human cases worldwide and for all major foodborne outbreaks in Europe and North America since
1271-760: The host cytoplasm , multiple changes in bacterial metabolism and gene expression help to complete its metamorphosis from soil dweller to intracellular pathogen. Studies suggest that up to 10% of human gastrointestinal tracts may be colonized by L. monocytogenes . Nevertheless, clinical diseases due to L. monocytogenes are more frequently recognized by veterinarians , especially as meningoencephalitis in ruminants . See: listeriosis in animals . Due to its frequent pathogenicity , causing meningitis in newborns (acquired transvaginally), pregnant women are often advised not to eat soft cheeses such as Brie , Camembert , feta , and queso blanco fresco , which may be contaminated with and permit growth of L. monocytogenes . It
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1312-411: The host cell walls. These host cells are generally M cells and Peyer's patches of the intestinal mucosa. Once attached to these cells, L. monocytogenes can translocate past the intestinal membrane and into the body. . Alternatively, losses of structural integrity (such as small lacerations) in the gastrointestinal epithelium could allow the microorganism to penetrate from the gastrointestinal tract to
1353-399: The host, induces the expression of virulence genes in L. monocytogenes . Little is known about how this bacterium switches between acting as a saprophyte and a pathogen; however, several noncoding RNAs are thought to be required to induce this change. L. monocytogenes has three distinct lineages, with differing evolutionary histories and pathogenic potentials. Lineage I strains contain
1394-402: The majority of human clinical isolates and all human epidemic clones, but are underrepresented in animal clinical isolates. Lineage II strains are overrepresented in animal cases and underrepresented in human clinical cases, and are more prevalent in environmental and food samples . Lineage III isolates are very rare, but significantly more common in animal than human isolates. The Anton test
1435-726: The order Bacillales, which also includes Bacillus and Staphylococcus . Listeria currently contains 27 species: Listeria aquatica, Listeria booriae, Listeria cornellensis, Listeria cossartiae, Listeria costaricensis, Listeria farberi, Listeria fleischmannii, Listeria floridensis, Listeria goaensis, Listeria grandensis, Listeria grayi, Listeria immobilis, Listeria innocua, Listeria ivanovii, Listeria marthii, Listeria monocytogenes, Listeria murrayi, Listeria newyorkensis, Listeria portnoyi, Listeria riparia, Listeria rocourtiae, Listeria rustica, Listeria seeligeri, Listeria thailandensis, Listeria valentina, Listeria weihenstephanensis, Listeria welshimeri . L. denitrificans , previously thought to be part of
1476-671: The organisms in foods. When listeric meningitis occurs, the overall mortality may reach 70%, from sepsis 50%, and from perinatal/neonatal infections greater than 80%. In infections during pregnancy, the mother usually survives. Reports of successful treatment with parenteral penicillin or ampicillin exist. Trimethoprim-sulfamethoxazole has been shown effective in patients allergic to penicillin. A bacteriophage, Listeria phage P100 , has been proposed as food additive to control L. monocytogenes . Bacteriophage treatments have been developed by several companies. EBI Food Safety and Intralytix both have products suitable for treatment of
1517-456: The placental blood barrier through the combined binding of both InIA and InIB to host cells. Research has shown that only the combined binding of these two virulence factors allows the bacteria to cross that barrier. This is not the case in mice and guinea pigs whose cells only have affinity for InIB in mice and InIA in guinea pigs. InlB blocks the action of cytotoxic T-cells and extents the infected cell lifespan. This cell biology article
1558-614: The tissue is kept at 4 °C for some days before inoculation into bacteriologic media. The organism is a facultative anaerobe and is catalase-positive and motile. Listeria produces acid, but not gas, when fermenting a variety of carbohydrates. The motility at room temperature and hemolysin production are primary findings that help differentiate Listeria from Corynebacterium . The methods for analysis of food are complex and time-consuming. The present U.S. FDA method, revised in September 1990, requires 24 and 48 hours of enrichment, followed by
1599-487: The up-regulation of several virulence genes. Depending on the location of the bacterium within the host organism, different activators up-regulate the virulence genes. SigB, an alternative sigma factor , up-regulates Vir genes in the intestines, whereas PrfA up-regulates gene expression when the bacterium is present in blood. L. monocytogenes also senses the entry to host by examining available nutrient sources. For example L-glutamine , an abundant nitrogen source in
1640-478: The whole body — and may suffer from physical retardation. Influenza -like symptoms, including persistent fever, usually precede the onset of the aforementioned disorders. Gastrointestinal symptoms, such as nausea, vomiting, and diarrhea , may precede more serious forms of listeriosis or may be the only symptoms expressed. Gastrointestinal symptoms were epidemiologically associated with use of antacids or cimetidine . The onset time to serious forms of listeriosis
1681-505: Was not identified as a cause of foodborne illness until 1981, however. An outbreak of listeriosis in Halifax , Nova Scotia , involving 41 cases and 18 deaths, mostly in pregnant women and neonates, was epidemiologically linked to the consumption of coleslaw containing cabbage that had been contaminated with L. monocytogenes -contaminated sheep manure. Since then, a number of cases of foodborne listeriosis have been reported, and L. monocytogenes