An immunoassay ( IA ) is a biochemical test that measures the presence or concentration of a macromolecule or a small molecule in a solution through the use of an antibody (usually) or an antigen (sometimes). The molecule detected by the immunoassay is often referred to as an " analyte " and is in many cases a protein , although it may be other kinds of molecules, of different sizes and types, as long as the proper antibodies that have the required properties for the assay are developed. Analytes in biological liquids such as serum or urine are frequently measured using immunoassays for medical and research purposes.
24-610: Eia or EIA may refer to: Medicine [ edit ] Enzyme immunoassay Equine infectious anemia Exercise-induced anaphylaxis Exercise-induced asthma External iliac artery Transport [ edit ] Edmonton International Airport , in Alberta, Canada Erbil International Airport , in Kurdistan Region, Iraq Evergreen International Airlines , an American airline Other uses [ edit ] Eia ,
48-510: A British environmental organisation Equity-indexed annuity European Inventor Award , awarded annually by the European Patent Office International Anticommunist Entente (French: Entente Internationale Anticommuniste ) People with the surname [ edit ] Harald Eia (born 1966), Norwegian comedian Magnhild Eia (born 1960), Norwegian politician Topics referred to by
72-541: A color change in a solution, fluoresce under light, or can be induced to emit light. Rosalyn Sussman Yalow and Solomon Berson are credited with the development of the first immunoassays in the 1950s. Yalow accepted the Nobel Prize for her work in immunoassays in 1977, becoming the second American woman to have won the award. Immunoassays became considerably simpler to perform and more popular when techniques for chemically linked enzymes to antibodies were demonstrated in
96-417: A competitive, homogeneous immunoassay, unlabelled analyte in a sample competes with labelled analyte to bind an antibody. In the heterogeneous assays, the labelled, unbound analyte is separated or washed away, and the remaining labelled, bound analyte is measured. Mixing a sample with labelled antibodies, the targeted analyte is bound by labelled antibodies. The unbound, labelled antibodies are washed away, and
120-459: A complex mixture of macromolecules. In immunology the particular macromolecule bound by an antibody is referred to as an antigen and the area on an antigen to which the antibody binds is called an epitope . In some cases, an immunoassay may use an antigen to detect for the presence of antibodies, which recognize that antigen, in a solution. In other words, in some immunoassays, the analyte may be an antibody rather than an antigen. In addition to
144-483: A former Medieval manor that is now part of Central London Electronic Industries Alliance , formerly Electronic Industries Association, an American trade organization EIA standards Emerging issues analysis Emirates Investment Authority Energy Information Administration of the United States Department of Energy Environmental impact assessment Environmental Investigation Agency ,
168-443: A number of modern immunoassays. Protein microarrays are a type of immunoassay that often employ fluorogenic reporters. Some labels work via electrochemiluminescence (ECL), in which the label emits detectable light in response to electric current. While some kind of label is generally employed in immunoassays, there are certain kinds of assays which do not rely on labels, but instead employ detection methods that do not require
192-457: A quick qualtitative immunoassay. Immunoassays are used in sports anti-doping laboratories to test athletes' blood samples for prohibited recombinant human growth hormone (rhGH, rGH, hGH, GH). The photoacoustic immunoassay measures low-frequency acoustic signals generated by metal nanoparticle tags. Illuminated by a modulated light at a plasmon resonance wavelength, the nanoparticles generate strong acoustic signal, which can be measured using
216-484: Is enzymes . Immunoassays which employ enzymes are referred to as enzyme immunoassays (EIAs), of which enzyme-linked immunosorbent assays (ELISAs) and enzyme multiplied immunoassay technique (EMIT) are the most common types. Enzymes used in ELISAs include horseradish peroxidase (HRP), alkaline phosphatase (AP) or glucose oxidase . These enzymes allow for detection often because they produce an observable color change in
240-575: Is different from Wikidata All article disambiguation pages All disambiguation pages Enzyme immunoassay An enzyme immunoassay is any of several immunoassay methods that use an enzyme bound to an antigen or antibody. These may include: Enzyme-linked immunosorbent assay (ELISA) Enzyme multiplied immunoassay technique (EMIT) Fluorescent enzyme immunoassays (FEIAs) Chemiluminescent immunoassays (CLIAs) Radioimmunoassays (RIAs) [REDACTED] Index of articles associated with
264-555: Is present, using a lateral flow setup. The COVID-19 rapid antigen test is also a qualitative, lateral-flow test. Other clinical immunoassays are quantitative; they measure amounts. Immunoassays can measure levels of CK-MB to assess heart disease, insulin to assess hypoglycemia , prostate-specific antigen to detect prostate cancer , and some are also used for the detection and/or quantitative measurement of some pharmaceutical compounds (see Enzyme multiplied immunoassay technique for more details). Drug testing also starts with
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#1732848944190288-407: Is run. In a competitive, homogeneous immunoassay, unlabelled analyte in a sample competes with labeled analyte to bind an antibody. The amount of labelled, unbound analyte is then measured. In theory, the more analyte in the sample, the more labelled analyte gets displaced and then measured; hence, the amount of labelled, unbound analyte is proportional to the amount of analyte in the sample. As in
312-423: The analyte in question, and the concentration of that analyte is generally known. Comparison of an assay's response to a real sample against the assay's response produced by the calibrators makes it possible to interpret the signal strength in terms of the presence or concentration of analyte in the sample. Immunoassays rely on the ability of an antibody to recognize and bind a specific macromolecule in what might be
336-441: The analyte is not present in the unknown sample. This type of immunoassay is also known as a sandwich assay as the analyte is "sandwiched" between two antibodies. A wide range of medical tests are immunoassays, called immunodiagnostics in this context. Many home pregnancy tests are immunoassays, which detect the pregnancy marker human chorionic gonadotropin . More specifically, they are qualitative tests that detect whether hCG
360-424: The assay. Multi-step assays are often called separation immunoassays or heterogeneous immunoassays. Some immunoassays can be carried out simply by mixing the reagents and samples and making a physical measurement. Such assays are called homogeneous immunoassays, or less frequently non-separation immunoassays. The use of a calibrator is often employed in immunoassays. Calibrators are solutions that are known to contain
384-455: The binding of an antibody to its antigen, the other key feature of all immunoassays is a means to produce a measurable signal in response to the binding. Most, though not all, immunoassays involve chemically linking antibodies or antigens with some kind of detectable label. A large number of labels exist in modern immunoassays, and they allow for detection through different means. Many labels are detectable because they either emit radiation, produce
408-434: The bound, labelled antibodies are measured. The intensity of the signal is directly proportional to the amount of analyte in the sample. The analyte in the unknown sample is bound to the antibody site, then the labelled antibody is bound to the analyte. The amount of labelled antibody on the site is then measured. It will be directly proportional to the concentration of the analyte because the labelled antibody will not bind if
432-405: The commercial immunoassay industry earned US$ 17,000,000,000 and was thought to have prospects of slow annual growth in the 2 to 3 percent range. Immunoassays employ a variety of different labels to allow for detection of antibodies and antigens. Labels are typically chemically linked or conjugated to the desired antibody or antigen. Possibly one of the most popular labels to use in immunoassays
456-473: The earliest immunoassays developed, but have fallen out of favor largely due to the difficulty and potential dangers presented by working with radioactivity. A newer approach to immunoassays involves combining real-time quantitative polymerase chain reaction (RT qPCR) and traditional immunoassay techniques. Called real-time immunoquantitative PCR (iqPCR) the label used in these assays is a DNA probe. Fluorogenic reporters like phycoerythrin are used in
480-418: The late 1960s. In 1983, Professor Anthony Campbell at Cardiff University replaced radioactive iodine used in immunoassay with an acridinium ester that makes its own light: chemiluminescence . This type of immunoassay is now used in around 100 million clinical tests every year worldwide, enabling clinicians to measure a wide range of proteins, pathogens and other molecules in blood samples. By 2012,
504-457: The modification or labeling the components of the assay. Surface plasmon resonance is an example of technique that can detect binding between an unlabeled antibody and antigens. Another demonstrated labeless immunoassay involves measuring the change in resistance on an electrode as antigens bind to it. Immunoassays can be run in a number of different formats. Generally, an immunoassay will fall into one of several categories depending on how it
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#1732848944190528-456: The presence of certain reagents. In some cases these enzymes are exposed to reagents which cause them to produce light or chemiluminescence. There are several types of ELISA: direct, indirect, sandwich, competitive. Radioactive isotopes can be incorporated into immunoassay reagents to produce a radioimmunoassay (RIA). Radioactivity emitted by bound antibody-antigen complexes can be easily detected using conventional methods. RIAs were some of
552-741: The same name This set index article includes a list of related items that share the same name (or similar names). If an internal link incorrectly led you here, you may wish to change the link to point directly to the intended article. Retrieved from " https://en.wikipedia.org/w/index.php?title=Enzyme_immunoassay&oldid=1129581699 " Category : Set index articles Hidden categories: Articles with short description Short description with empty Wikidata description All set index articles Immunoassay Immunoassays come in many different formats and variations. Immunoassays may be run in multiple steps with reagents being added and washed away or separated at different points in
576-502: The same term [REDACTED] This disambiguation page lists articles associated with the title EIA . If an internal link led you here, you may wish to change the link to point directly to the intended article. Retrieved from " https://en.wikipedia.org/w/index.php?title=EIA&oldid=987072605 " Categories : Disambiguation pages Disambiguation pages with surname-holder lists Hidden categories: Articles containing French-language text Short description
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