197-532: Population figures for the Indigenous peoples of the Americas before European colonization have been difficult to establish. Estimates have varied widely from as low as 8 million to as many as 100 million, though many scholars gravitated toward an estimate of around 50 million by the end of the 20th century. The monarchs of the nascent Spanish Empire decided to fund Christopher Columbus' voyage in 1492 , leading to
394-460: A DNA study. While it is difficult to determine exactly how many Natives lived in North America before Columbus, estimates range from 3.8 million, as mentioned above, to 7 million people to a high of 18 million. Scholars vary on the estimated size of the Indigenous population in what is now Canada prior to colonization and on the effects of European contact . During the late 15th century
591-481: A collective noun (also describing First Nations, Inuit, and Métis) is a specific term of art used in some legal documents, including the Constitution Act, 1982 . Over time, as societal perceptions and government–indigenous relationships have shifted, many historical terms have changed definitions or been replaced as they have fallen out of favor. The use of the term "Indian" is frowned upon because it represents
788-445: A buffer to recruit or titrate ions or antibiotics. Extracellular DNA acts as a functional extracellular matrix component in the biofilms of several bacterial species. It may act as a recognition factor to regulate the attachment and dispersal of specific cell types in the biofilm; it may contribute to biofilm formation; and it may contribute to the biofilm's physical strength and resistance to biological stress. Cell-free fetal DNA
985-459: A cell makes up its genome ; the human genome has approximately 3 billion base pairs of DNA arranged into 46 chromosomes. The information carried by DNA is held in the sequence of pieces of DNA called genes . Transmission of genetic information in genes is achieved via complementary base pairing. For example, in transcription, when a cell uses the information in a gene, the DNA sequence is copied into
1182-588: A colonial state. The peopling of the Americas began when Paleolithic hunter-gatherers ( Paleo-Indians ) entered North America from the North Asian Mammoth steppe via the Beringia land bridge , which had formed between northeastern Siberia and western Alaska due to the lowering of sea level during the Last Glacial Maximum (26,000 to 19,000 years ago). These populations expanded south of
1379-445: A complementary RNA sequence through the attraction between the DNA and the correct RNA nucleotides. Usually, this RNA copy is then used to make a matching protein sequence in a process called translation , which depends on the same interaction between RNA nucleotides. In an alternative fashion, a cell may copy its genetic information in a process called DNA replication . The details of these functions are covered in other articles; here
1576-640: A connection to residential schools. Additionally, Indigenous respondents with higher HLS and HLASS scores had one or more negative health outcomes. While there are many studies that found an association between IHT and adverse health outcomes, scholars continue to suggest that it remains difficult to understand the impact of IHT. IHT needs to be systematically measured. Indigenous people also need to be understood in separate categories based on similar experiences, location, and background as opposed to being categorized as one monolithic group. For thousands of years, Indigenous peoples domesticated, bred, and cultivated
1773-492: A double helix can thus be pulled apart like a zipper, either by a mechanical force or high temperature . As a result of this base pair complementarity, all the information in the double-stranded sequence of a DNA helix is duplicated on each strand, which is vital in DNA replication. This reversible and specific interaction between complementary base pairs is critical for all the functions of DNA in organisms. Most DNA molecules are actually two polymer strands, bound together in
1970-511: A factor of 10 over 800–1,000 years. The data also show that there have been genetic exchanges between Asia, the Arctic and Greenland since the initial peopling of the Americas . A new study in early 2018 suggests that the effective population size of the original founding population of Native Americans was about 250 people. Early explanations for the population decline of the Indigenous peoples of
2167-835: A few still considered uncontacted peoples . The Americas also host millions of individuals of mixed Indigenous, European, and sometimes African or Asian descent, historically referred to as mestizos in Spanish-speaking countries. In many Latin American nations, people of partial Indigenous descent constitute a majority or significant portion of the population, particularly in Central America , Mexico , Peru, Bolivia, Ecuador , Colombia , Venezuela , Chile , and Paraguay. Mestizos outnumber Indigenous peoples in most Spanish-speaking countries, according to estimates of ethnic cultural identification. However, since Indigenous communities in
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#17328592882642364-428: A full set of the mitochondrial genes. Each human mitochondrion contains, on average, approximately 5 such mtDNA molecules. Each human cell contains approximately 100 mitochondria, giving a total number of mtDNA molecules per human cell of approximately 500. However, the amount of mitochondria per cell also varies by cell type, and an egg cell can contain 100,000 mitochondria, corresponding to up to 1,500,000 copies of
2561-1236: A genocide. No other way to describe it. And that's the way it needs to be described in the history books." Indigenous peoples of the Americas In the Americas , indigenous peoples comprise the two continents' pre-Columbian inhabitants prior to European settlement in the 15th century, as well as the ethnic groups that identify with the pre-Columbian population of the Americas as such. These populations exhibit significant diversity; some Indigenous peoples were historically hunter-gatherers , while others practiced agriculture and aquaculture . Various Indigenous societies developed complex social structures , including pre-contact monumental architecture, organized cities , city-states , chiefdoms , states , kingdoms , republics , confederacies , and empires . These societies possessed varying levels of knowledge in fields such as engineering , architecture , mathematics , astronomy , writing , physics , medicine , agriculture , irrigation , geology , mining , metallurgy , art , sculpture , and goldsmithing . Indigenous peoples continue to inhabit many regions of
2758-439: A helical fashion by noncovalent bonds; this double-stranded (dsDNA) structure is maintained largely by the intrastrand base stacking interactions, which are strongest for G,C stacks. The two strands can come apart—a process known as melting—to form two single-stranded DNA (ssDNA) molecules. Melting occurs at high temperatures, low salt and high pH (low pH also melts DNA, but since DNA is unstable due to acid depurination, low pH
2955-571: A higher number is also possible but this would be against the natural principle of least effort . The phosphate groups of DNA give it similar acidic properties to phosphoric acid and it can be considered as a strong acid . It will be fully ionized at a normal cellular pH, releasing protons which leave behind negative charges on the phosphate groups. These negative charges protect DNA from breakdown by hydrolysis by repelling nucleophiles which could hydrolyze it. Pure DNA extracted from cells forms white, stringy clumps. The expression of genes
3152-417: A large array of plant species. These species now constitute between 50% and 60% of all crops in cultivation worldwide. In certain cases, the Indigenous peoples developed entirely new species and strains through artificial selection , as with the domestication and breeding of maize from wild teosinte grasses in the valleys of southern Mexico. Numerous such agricultural products retain their native names in
3349-424: A large citizenry. Indigenous civilizations also displayed impressive accomplishments in astronomy and mathematics, including the most accurate calendar in the world. The domestication of maize or corn required thousands of years of selective breeding, and continued cultivation of multiple varieties was done with planning and selection, generally by women. Inuit, Yupik, Aleut, and Indigenous creation myths tell of
3546-667: A long-standing puzzle known as the " C-value enigma ". However, some DNA sequences that do not code protein may still encode functional non-coding RNA molecules, which are involved in the regulation of gene expression . Some noncoding DNA sequences play structural roles in chromosomes. Telomeres and centromeres typically contain few genes but are important for the function and stability of chromosomes. An abundant form of noncoding DNA in humans are pseudogenes , which are copies of genes that have been disabled by mutation. These sequences are usually just molecular fossils , although they can occasionally serve as raw genetic material for
3743-467: A lower genetic diversity than populations from other continental regions. Decreasing genetic diversity with increasing geographic distance from the Bering Strait can be seen, as well as a decreasing genetic similarity to Siberian populations from Alaska (genetic entry point). A higher level of diversity and lower level of population structure in western South America compared to eastern South America
3940-544: A multitude of ways because the Indigenous community and their history are diverse. Many studies (such as Whitbeck et al., 2014; Brockie, 2012; Anastasio et al., 2016; Clark & Winterowd, 2012; Tucker et al., 2016) have evaluated the impact of IHT on health outcomes of Indigenous communities from the United States and Canada. IHT is a difficult term to standardize and measure because of the vast and variable diversity of Indigenous people and their communities. Therefore, it
4137-409: A narrower, deeper major groove. The A form occurs under non-physiological conditions in partly dehydrated samples of DNA, while in the cell it may be produced in hybrid pairings of DNA and RNA strands, and in enzyme-DNA complexes. Segments of DNA where the bases have been chemically modified by methylation may undergo a larger change in conformation and adopt the Z form . Here, the strands turn about
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#17328592882644334-416: A pre-Columbian high of an estimated three million to some 300,000 in 1997. The Spanish Empire and other Europeans re-introduced horses to the Americas. Some of these animals escaped and began to breed and increase their numbers in the wild. The reintroduction of the horse , extinct in the Americas for over 7500 years, had a profound impact on Indigenous cultures in several regions, such as those of
4531-431: A process called translation . Within eukaryotic cells, DNA is organized into long structures called chromosomes . Before typical cell division , these chromosomes are duplicated in the process of DNA replication, providing a complete set of chromosomes for each daughter cell. Eukaryotic organisms ( animals , plants , fungi and protists ) store most of their DNA inside the cell nucleus as nuclear DNA , and some in
4728-442: A radius of 10 Å (1.0 nm). According to another study, when measured in a different solution, the DNA chain measured 22–26 Å (2.2–2.6 nm) wide, and one nucleotide unit measured 3.3 Å (0.33 nm) long. The buoyant density of most DNA is 1.7g/cm . DNA does not usually exist as a single strand, but instead as a pair of strands that are held tightly together. These two long strands coil around each other, in
4925-416: A second protein when read in the opposite direction along the other strand. In bacteria , this overlap may be involved in the regulation of gene transcription, while in viruses, overlapping genes increase the amount of information that can be encoded within the small viral genome. DNA can be twisted like a rope in a process called DNA supercoiling . With DNA in its "relaxed" state, a strand usually circles
5122-520: A second, more recent wave of migration several thousand years later and have much more recent genetic and cultural commonalities with the Indigenous peoples of Siberia . However, these groups are nonetheless considered "Indigenous peoples of the Americas". The term Amerindian , a portmanteau of "American Indian", was coined in 1902 by the American Anthropological Association . It has been controversial ever since its creation. It
5319-445: A simple TTAGGG sequence. These guanine-rich sequences may stabilize chromosome ends by forming structures of stacked sets of four-base units, rather than the usual base pairs found in other DNA molecules. Here, four guanine bases, known as a guanine tetrad , form a flat plate. These flat four-base units then stack on top of each other to form a stable G-quadruplex structure. These structures are stabilized by hydrogen bonding between
5516-601: A threat to livestock. Several bird species, such as turkeys , Muscovy ducks , Puna ibis , and neotropic cormorants were domesticated by various peoples in Mesoamerica and South America to be used for poultry. In the Andean region, indigenous peoples domesticated llamas and alpacas to produce fiber and meat. The llama was the only beast of burden in the Americas before European colonization. Guinea pigs were domesticated from wild cavies to be raised for meat consumption in
5713-497: A twenty-five percent to eighty percent Indigenous population decrease post-contact. Roland G Robertson suggests that during the late 1630s, smallpox killed over half of the Wyandot (Huron) , who controlled most of the early North American fur trade in the area of New France . In 1871 there was an enumeration of the Indigenous population within the limits of Canada at the time, showing a total of only 102,358 individuals. From 2006 to 2016,
5910-493: A variety of origins of their respective peoples. Some were "always there" or were created by gods or animals, some migrated from a specified compass point , and others came from "across the ocean". The European colonization of the Americas fundamentally changed the lives and cultures of the resident Indigenous peoples. Although the exact pre-colonization population count of the Americas is unknown, scholars estimate that Indigenous populations diminished by between 80% and 90% during
6107-642: A violent poison. Eventually, a Taíno Cacique named Enriquillo managed to hold out in the Baoruco Mountain Range for thirteen years, causing serious damage to the Spanish, Carib-held plantations and their Indian auxiliaries . Hearing of the seriousness of the revolt, Emperor Charles V (also King of Spain) sent Captain Francisco Barrionuevo to negotiate a peace treaty with the ever-increasing number of rebels. Two months later, after consultation with
Population history of the Indigenous peoples of the Americas - Misplaced Pages Continue
6304-437: A whole, in general, most Indigenous peoples prefer to be identified by the name of their specific nation, tribe, or band. Early settlers often adopted terms that some tribes used for each other, not realizing these were derogatory terms used by enemies. When discussing broader subsets of peoples, naming has often been based on shared language, region, or historical relationship. Many English exonyms have been used to refer to
6501-528: A whole. Since the 1970s, the word "Indigenous", which is capitalized when referring to people, has gradually emerged as a favored umbrella term. The capitalization is to acknowledge that Indigenous peoples have cultures and societies that are equal to Europeans, Africans, and Asians. This has recently been acknowledged in the AP Stylebook . Some consider it improper to refer to Indigenous people as "Indigenous Americans" or to append any colonial nationality to
6698-524: Is a common term, though nativos or pueblos nativos ('native peoples') may also be heard; moreover, aborigen ('aborigine') is used in Argentina and pueblos originarios ('original peoples') is common in Chile . In Brazil, indígenas and povos originários ('Indigenous peoples') are common formal-sounding designations, while índio ('Indian') is still the more often heard term (the noun for
6895-416: Is an arduous task to assign an operational definition and systematically collect data when studying IHT. Many of the studies that incorporate IHT measure it in different ways, making it hard to compile data and review it holistically. This is an important point that provides context for the following studies that attempt to understand the relationship between IHT and potential adverse health impacts. Some of
7092-447: Is called intercalation . Most intercalators are aromatic and planar molecules; examples include ethidium bromide , acridines , daunomycin , and doxorubicin . For an intercalator to fit between base pairs, the bases must separate, distorting the DNA strands by unwinding of the double helix. This inhibits both transcription and DNA replication, causing toxicity and mutations. As a result, DNA intercalators may be carcinogens , and in
7289-435: Is called a polynucleotide . The backbone of the DNA strand is made from alternating phosphate and sugar groups. The sugar in DNA is 2-deoxyribose , which is a pentose (five- carbon ) sugar. The sugars are joined by phosphate groups that form phosphodiester bonds between the third and fifth carbon atoms of adjacent sugar rings. These are known as the 3′-end (three prime end), and 5′-end (five prime end) carbons,
7486-445: Is composed of one of four nitrogen-containing nucleobases ( cytosine [C], guanine [G], adenine [A] or thymine [T]), a sugar called deoxyribose , and a phosphate group . The nucleotides are joined to one another in a chain by covalent bonds (known as the phosphodiester linkage ) between the sugar of one nucleotide and the phosphate of the next, resulting in an alternating sugar-phosphate backbone . The nitrogenous bases of
7683-434: Is dependent on ionic strength and the concentration of DNA. As a result, it is both the percentage of GC base pairs and the overall length of a DNA double helix that determines the strength of the association between the two strands of DNA. Long DNA helices with a high GC -content have more strongly interacting strands, while short helices with high AT content have more weakly interacting strands. In biology, parts of
7880-500: Is estimated to have been between 200,000 and two million, with a figure of 500,000 currently accepted by Canada's Royal Commission on Aboriginal Health. Although not without conflict, European Canadians ' early interactions with First Nations and Inuit populations were relatively peaceful. However repeated outbreaks of European infectious diseases such as influenza, measles, and smallpox (to which they had no natural immunity), combined with other effects of European contact, resulted in
8077-540: Is general agreement that the Americas were first settled from Asia, the pattern of migration and the place(s) of origin in Eurasia of the peoples who migrated to the Americas remain unclear. The traditional theory is that Ancient Beringians moved when sea levels were significantly lowered due to the Quaternary glaciation , following herds of now-extinct Pleistocene megafauna along ice-free corridors that stretched between
Population history of the Indigenous peoples of the Americas - Misplaced Pages Continue
8274-526: Is general agreement that war became deadlier after the arrival of the Europeans and their firearms. The South or Central American infrastructure allowed for thousands of European conquistadors and tens of thousands of their Indian auxiliaries to attack the dominant Indigenous civilization. Empires such as the Incas depended on a highly centralized administration for the distribution of resources. Disruption caused by
8471-411: Is influenced by how the DNA is packaged in chromosomes, in a structure called chromatin . Base modifications can be involved in packaging, with regions that have low or no gene expression usually containing high levels of methylation of cytosine bases. DNA packaging and its influence on gene expression can also occur by covalent modifications of the histone protein core around which DNA is wrapped in
8668-432: Is introduced by enzymes called topoisomerases . These enzymes are also needed to relieve the twisting stresses introduced into DNA strands during processes such as transcription and DNA replication . DNA exists in many possible conformations that include A-DNA , B-DNA , and Z-DNA forms, although only B-DNA and Z-DNA have been directly observed in functional organisms. The conformation that DNA adopts depends on
8865-434: Is nothing more than a scholarly article of faith... In contrast, historian Russel Thornton has pointed out that there were disastrous epidemics and population losses during the first half of the sixteenth century "resulting from incidental contact, or even without direct contact, as disease spread from one American Indian tribe to another." Thornton has also challenged higher Indigenous population estimates, which are based on
9062-422: Is nothing special about the four natural nucleobases that evolved on Earth. On the other hand, DNA is tightly related to RNA which does not only act as a transcript of DNA but also performs as molecular machines many tasks in cells. For this purpose it has to fold into a structure. It has been shown that to allow to create all possible structures at least four bases are required for the corresponding RNA , while
9259-572: Is now Brazil, and 12 million in the Inca Empire ), the lowest estimates give a population decrease from all causes of 80% by the end of the 17th century (nine million people in 1650). Latin America would match its 15th-century population early in the 19th century; it numbered 17 million in 1800, 30 million in 1850, 61 million in 1900, 105 million in 1930, 218 million in 1960, 361 million in 1980, and 563 million in 2005. In
9456-400: Is observed. A relative lack of differentiation between Mesoamerican and Andean populations is a scenario that implies coastal routes were easier than inland routes for migrating peoples ( Paleo-Indians ) to traverse. The overall pattern that is emerging suggests that the Americas were recently colonized by a small number of individuals (effective size of about 70–250), and then they grew by
9653-415: Is possible that documents relating to such acts were deliberately destroyed, or sanitized. By the middle of the 18th century, colonists had the knowledge and technology to attempt biological warfare with the smallpox virus. They well understood the concept of quarantine, and that contact with the sick could infect the healthy with smallpox, and those who survived the illness would not be infected again. Whether
9850-514: Is rarely used). The stability of the dsDNA form depends not only on the GC -content (% G,C basepairs) but also on sequence (since stacking is sequence specific) and also length (longer molecules are more stable). The stability can be measured in various ways; a common way is the melting temperature (also called T m value), which is the temperature at which 50% of the double-strand molecules are converted to single-strand molecules; melting temperature
10047-428: Is recreated by an enzyme called DNA polymerase . This enzyme makes the complementary strand by finding the correct base through complementary base pairing and bonding it onto the original strand. As DNA polymerases can only extend a DNA strand in a 5′ to 3′ direction, different mechanisms are used to copy the antiparallel strands of the double helix. In this way, the base on the old strand dictates which base appears on
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#173285928826410244-428: Is the sequence of these four nucleobases along the backbone that encodes genetic information. RNA strands are created using DNA strands as a template in a process called transcription , where DNA bases are exchanged for their corresponding bases except in the case of thymine (T), for which RNA substitutes uracil (U). Under the genetic code , these RNA strands specify the sequence of amino acids within proteins in
10441-516: Is the largest human chromosome with approximately 220 million base pairs , and would be 85 mm long if straightened. In eukaryotes , in addition to nuclear DNA , there is also mitochondrial DNA (mtDNA) which encodes certain proteins used by the mitochondria. The mtDNA is usually relatively small in comparison to the nuclear DNA. For example, the human mitochondrial DNA forms closed circular molecules, each of which contains 16,569 DNA base pairs, with each such molecule normally containing
10638-486: Is to allow the cell to replicate chromosome ends using the enzyme telomerase , as the enzymes that normally replicate DNA cannot copy the extreme 3′ ends of chromosomes. These specialized chromosome caps also help protect the DNA ends, and stop the DNA repair systems in the cell from treating them as damage to be corrected. In human cells , telomeres are usually lengths of single-stranded DNA containing several thousand repeats of
10835-557: The Archaic period of North American cultures. Technology had advanced to the point where pottery had started to become common and the small-scale felling of trees had become feasible. Concurrently, the Archaic Indigenous peoples began using fire in a controlled manner. They carried out the intentional burning of vegetation to mimic the effects of natural fires that tended to clear forest understories. It made travel easier and facilitated
11032-616: The Beaver Wars . According to the Cambridge World History , the Oxford Handbook of Genocide Studies, and the Cambridge World History of Genocide, colonial policies in some cases included the deliberate genocide of indigenous peoples in North America. According to the Cambridge World History of Genocide, Spanish colonization of the Americas also included genocidal massacres. According to Adam Jones , genocidal methods included
11229-622: The Black Death if it were not for the constant enslavement they were subject to. He further contends that enslavement of Native Americans was in fact the primary cause of their depopulation in Spanish territories; that the majority of Indians enslaved were women and children compared to the enslavement of Africans which mostly targeted adult males and in turn they were sold at a 50% to 60% higher price, and that 2,462,000 to 4,985,000 Amerindians were enslaved between Columbus's arrival and 1900. Throughout history, Indigenous people have been subjected to
11426-597: The Chiribaya to herd llamas . The vast majority of indigenous dog breeds in the Americas went extinct, due to being replaced by dogs of European origin. The Fuegian dog was a domesticated variation of the culpeo that was raised by several cultures in Tierra del Fuego , like the Selk'nam and the Yahgan . It was exterminated by Argentine and Chilean settlers, due to supposedly posing as
11623-657: The DNA sequence . Mutagens include oxidizing agents , alkylating agents and also high-energy electromagnetic radiation such as ultraviolet light and X-rays . The type of DNA damage produced depends on the type of mutagen. For example, UV light can damage DNA by producing thymine dimers , which are cross-links between pyrimidine bases. On the other hand, oxidants such as free radicals or hydrogen peroxide produce multiple forms of damage, including base modifications, particularly of guanosine, and double-strand breaks. A typical human cell contains about 150,000 bases that have suffered oxidative damage. Of these oxidative lesions,
11820-733: The Great Plains , the Northwest Plateau , the Great Basin , Aridoamerica , the Gran Chaco and the Southern Cone . By domesticating horses, some tribes had great success: horses enabled them to expand their territories, exchange more goods with neighboring tribes, and more easily capture game , such as bison . According to Erin McKenna and Scott L. Pratt, the Indigenous population of the Americas
12017-496: The Inca Civil War of 1529–1532. Smallpox was only the first epidemic. Typhus (probably) in 1546, influenza and smallpox together in 1558, smallpox again in 1589, diphtheria in 1614, and measles in 1618—all ravaged the remains of Inca culture. Smallpox killed millions of native inhabitants of Mexico. Unintentionally introduced at Veracruz with the arrival of Pánfilo de Narváez on 23 April 1520, smallpox ravaged Mexico in
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#173285928826412214-562: The Laurentide and Cordilleran ice sheets. Another route proposed is that, either on foot or using boats , they migrated down the Pacific coast to South America as far as Chile . Any archaeological evidence of coastal occupation during the last Ice Age would now have been covered by the sea level rise , up to a hundred metres since then. While technically referring to the era before Christopher Columbus ' voyages of 1492 to 1504, in practice
12411-540: The Laurentide Ice Sheet and spread rapidly southward, occupying both North and South America by 12,000 to 14,000 years ago. The earliest populations in the Americas, before roughly 10,000 years ago, are known as Paleo-Indians . Indigenous peoples of the Americas have been linked to Siberian populations by proposed linguistic factors , the distribution of blood types , and in genetic composition as reflected by molecular data, such as DNA . While there
12608-790: The Menominee of Wisconsin—suggest that longstanding "sacred values" may represent a summary of sustainable millennial traditions. Numerous Native American dog breeds have been used by the people of the Americas, such as the Canadian Eskimo dog , the Carolina dog , and the Chihuahua . Some indigenous peoples in the Great Plains used dogs for pulling travois , while others like the Tahltan bear dog were bred to hunt larger game. Some Andean cultures also bred
12805-685: The Seven Years' War , Colonel Henry Bouquet ordered his men to take smallpox-infested blankets from their hospital and gave them as gifts to two neutral Lenape Indian dignitaries during a peace settlement negotiation, according to the entry in the Captain's ledger, "To convey the Smallpox to the Indians". In the following weeks, Sir Jeffrey Amherst conspired with Bouquet to "Extirpate this Execreble Race" of Native Americans, writing, "Could it not be contrived to send
13002-498: The Taínos . The second European explanation was a perceived divine approval, in which God removed the Indigenous peoples as part of His "divine plan" to make way for a new Christian civilization. Many Native Americans viewed their troubles in a religious framework within their own belief systems. According to later academics such as Noble David Cook, a community of scholars began "quietly accumulating piece by piece data on early epidemics in
13199-406: The amino-acid sequences of proteins is determined by the rules of translation , known collectively as the genetic code . The genetic code consists of three-letter 'words' called codons formed from a sequence of three nucleotides (e.g. ACT, CAG, TTT). In transcription, the codons of a gene are copied into messenger RNA by RNA polymerase . This RNA copy is then decoded by a ribosome that reads
13396-632: The encomienda system). The cattle introduced by the Spanish contaminated various water reserves which Native Americans dug in the fields to accumulate rainwater. In response, the Franciscans and Dominicans created public fountains and aqueducts to guarantee access to drinking water . But when the Franciscans lost their privileges in 1572, many of these fountains were no longer guarded and so deliberate well poisoning may have happened. Although no proof of such poisoning has been found, some historians believe
13593-617: The establishment of colonies and marking the beginning of the migration of millions of Europeans and Africans to the Americas. While the population of European settlers, primarily from Spain, Portugal, France, England, and the Netherlands, along with African slaves, grew steadily, the Indigenous population plummeted. There are numerous reasons for the population decline, including exposure to Eurasian diseases such as influenza , pneumonic plagues , and smallpox ; direct violence by settlers and their allies through war and forced removal ; and
13790-477: The history and prehistory of the Americas before the appearance of significant European and African influences on the American continents, spanning the time of the original arrival in the Upper Paleolithic to European colonization during the early modern period . The Norte Chico civilization (in present-day Peru) is one of the defining six original civilizations of the world, arising independently around
13987-420: The mitochondria as mitochondrial DNA or in chloroplasts as chloroplast DNA . In contrast, prokaryotes ( bacteria and archaea ) store their DNA only in the cytoplasm , in circular chromosomes . Within eukaryotic chromosomes, chromatin proteins, such as histones , compact and organize DNA. These compacting structures guide the interactions between DNA and other proteins, helping control which parts of
14184-448: The residential school ancestry studies ask respondents if their parents, grandparents, great-grandparents, or "elders from their community" went to a residential school to understand if family or community history in residential schools is associated with negative health outcomes. In a comprehensive review of the research literature, Joseph Gone and colleagues compiled and compared outcomes for studies using these IHT measures relative to
14381-431: The " West Indies " (or " Antilles "), a name that is still used to describe the islands. This led to the blanket term "Indies" and "Indians" ( Spanish : indios ; Portuguese : índios ; French : indiens ; Dutch : indianen ) for the Indigenous inhabitants, which implied some kind of ethnic or cultural unity among the Indigenous peoples of the Americas. This unifying concept, codified in law, religion, and politics,
14578-477: The "great dying". According to one of the researchers, UCL Geography Professor Mark Maslin , the large death toll also boosted the economies of Europe: "the depopulation of the Americas may have inadvertently allowed the Europeans to dominate the world. It also allowed for the Industrial Revolution and for Europeans to continue that domination." When Old World diseases were first carried to the Americas at
14775-603: The 1520s, possibly killing over 150,000 in Tenochtitlán (the heartland of the Aztec Empire) alone, and aiding in the victory of Hernán Cortés over the Aztec Empire at Tenochtitlan (present-day Mexico City) in 1521. There are many factors as to why Indigenous peoples suffered such immense losses from Afro-Eurasian diseases. Many Old World diseases, like cow pox, are acquired from domesticated animals that are not indigenous to
14972-577: The 16th century were Constantinople and Paris with 300,000 and 200,000 inhabitants respectively. The population in London, Madrid, and Rome hardly exceeded 50,000 people. In 1523, right around the time of the Spanish conquest, the entire population in the country of England was just under three million people. This fact speaks to the level of sophistication, agriculture, governmental procedure, and rule of law that existed in Tenochtitlan, needed to govern over such
15169-610: The 250,000 Taínos of Hispaniola , represented the dominant culture in the Greater Antilles and the Bahamas. Within thirty years about 70% of the Taínos had died. They had no immunity to European diseases, so outbreaks of measles and smallpox ravaged their population. One such outbreak occurred in a camp of enslaved Africans, where smallpox spread to the nearby Taíno population and reduced their numbers by 50%. Increasing punishment of
15366-419: The 3′ and 5′ carbons along the sugar-phosphate backbone confers directionality (sometimes called polarity) to each DNA strand. In a nucleic acid double helix , the direction of the nucleotides in one strand is opposite to their direction in the other strand: the strands are antiparallel . The asymmetric ends of DNA strands are said to have a directionality of five prime end (5′ ), and three prime end (3′), with
15563-588: The 5′ end having a terminal phosphate group and the 3′ end a terminal hydroxyl group. One major difference between DNA and RNA is the sugar, with the 2-deoxyribose in DNA being replaced by the related pentose sugar ribose in RNA. The DNA double helix is stabilized primarily by two forces: hydrogen bonds between nucleotides and base-stacking interactions among aromatic nucleobases. The four bases found in DNA are adenine ( A ), cytosine ( C ), guanine ( G ) and thymine ( T ). These four bases are attached to
15760-629: The Americas and their relation to the subjugation of native peoples." Scholars like Cook believe that widespread epidemic disease, to which the Indigenous peoples had no prior exposure or resistance, was the primary cause of the massive population decline of the Native Americans. One of the most devastating diseases was smallpox, but other deadly diseases included typhus , measles , influenza, bubonic plague , cholera , malaria , tuberculosis , mumps , yellow fever , and pertussis , which were chronic in Eurasia. However, recently scholars have studied
15957-462: The Americas are defined by cultural identification and kinship rather than ancestry or race , mestizos are typically not counted among the Indigenous population unless they speak an Indigenous language or identify with a specific Indigenous culture. Additionally, many individuals of wholly Indigenous descent who do not follow Indigenous traditions or speak an Indigenous language have been classified or self-identify as mestizo due to assimilation into
16154-519: The Americas have been more vocal about how they want to be addressed, pushing to suppress the use of terms widely considered to be obsolete, inaccurate, or racist . During the latter half of the 20th century and the rise of the Indian rights movement , the United States federal government responded by proposing the use of the term " Native American ", to recognize the primacy of Indigenous peoples' tenure in
16351-533: The Americas in 1492 was not necessarily at a high point and may actually have already been in decline in some areas. Indigenous populations in most areas of the Americas reached a low point by the early 20th century. Using an estimate of approximately 37 million people in Mexico, Central and South America in 1492 (including 6 million in the Aztec Empire , 5–10 million in the Mayan States, 11 million in what
16548-669: The Americas include the brutal practices of the Spanish conquistadores , as recorded by the Spaniards themselves, such as the encomienda system, which was ostensibly set up to protect people from warring tribes as well as to teach them the Spanish language and the Catholic religion , but in practice was tantamount to serfdom and slavery . The most notable account was that of the Dominican friar Bartolomé de las Casas , whose writings vividly depict Spanish atrocities committed in particular against
16745-993: The Americas, with significant populations in countries such as Bolivia , Canada , Chile , Colombia , Ecuador , Guatemala , Mexico , Peru , and the United States . There are at least 1,000 different indigenous languages spoken across the Americas, with 574 federally recognized tribes in the US alone. Some languages, including Quechua , Arawak , Aymara , Guaraní , Mayan , and Nahuatl , have millions of speakers and are recognized as official by governments in Bolivia , Peru , Paraguay , and Greenland . Indigenous peoples, whether residing in rural or urban areas, often maintain aspects of their cultural practices, including religion , social organization , and subsistence practices. Over time, these cultures have evolved, preserving traditional customs while adapting to modern needs. Some Indigenous groups remain relatively isolated from Western culture , with
16942-451: The Americas. European populations had adapted to these diseases, and built up resistance, over many generations. Many of the Old World diseases that were brought over to the Americas were diseases, like yellow fever , that were relatively manageable if infected as a child, but were deadly if infected as an adult. Children could often survive the disease, resulting in immunity to the disease for
17139-454: The Andean region. Guinea pigs are now widely raised in Western society as household pets. DNA Deoxyribonucleic acid ( / d iː ˈ ɒ k s ɪ ˌ r aɪ b oʊ nj uː ˌ k l iː ɪ k , - ˌ k l eɪ -/ ; DNA ) is a polymer composed of two polynucleotide chains that coil around each other to form a double helix . The polymer carries genetic instructions for
17336-505: The Audencia of Santo Domingo, Enriquillo was offered any part of the island to live in peace. The Laws of Burgos, 1512–1513 , were the first codified set of laws governing the behavior of Spanish settlers in America, particularly concerning Indigenous peoples. The laws forbade the maltreatment of them and endorsed their conversion to Catholicism . The Spanish crown found it difficult to enforce these laws in distant colonies. Epidemic disease
17533-435: The DNA are transcribed. DNA is a long polymer made from repeating units called nucleotides . The structure of DNA is dynamic along its length, being capable of coiling into tight loops and other shapes. In all species it is composed of two helical chains, bound to each other by hydrogen bonds . Both chains are coiled around the same axis, and have the same pitch of 34 ångströms (3.4 nm ). The pair of chains have
17730-453: The DNA double helix that need to separate easily, such as the TATAAT Pribnow box in some promoters , tend to have a high AT content, making the strands easier to pull apart. In the laboratory, the strength of this interaction can be measured by finding the melting temperature T m necessary to break half of the hydrogen bonds. When all the base pairs in a DNA double helix melt,
17927-570: The English and Spanish lexicons. The South American highlands became a center of early agriculture. Genetic testing of the wide variety of cultivars and wild species suggests that the potato has a single origin in the area of southern Peru , from a species in the Solanum brevicaule complex. Over 99% of all modern cultivated potatoes worldwide are descendants of a subspecies indigenous to south-central Chile , Solanum tuberosum ssp. tuberosum , where it
18124-476: The Europeans began the human trafficking of massive numbers of enslaved Western and Central African people to the Americas. Like Indigenous peoples, these African people, newly exposed to European diseases, lacked any inherited resistance to the diseases of Europe. In 1520, an African who had been infected with smallpox had arrived in Yucatán. By 1558, the disease had spread throughout South America and had arrived at
18321-401: The Indigenous peoples of the Americas. Some of these names were based on foreign language terms used by earlier explorers and colonists, while others resulted from the colonists' attempts to translate or transliterate endonyms from the native languages. Other terms arose during periods of conflict between the colonists and Indigenous peoples. Since the late 20th century, Indigenous peoples in
18518-709: The Indigenous population has grown by 42.5 percent, four times the national rate. According to the 2011 Canadian census , Indigenous peoples ( First Nations – 851,560, Inuit – 59,445 and Métis – 451,795) numbered at 1,400,685, or 4.3% of the country's total population. The population debate has often had ideological underpinnings. Low estimates were sometimes reflective of European notions of cultural and racial superiority . Historian Francis Jennings argued, "Scholarly wisdom long held that Indians were so inferior in mind and works that they could not possibly have created or sustained large populations." In 1998, Africanist Historian David Henige said many population estimates are
18715-538: The Indigenous population of North America in the first hundred years after the arrival of Europeans. Some 90 percent of the native population near Massachusetts Bay Colony died of smallpox in an epidemic in 1617–1619. In 1633, in Fort Orange (New Netherland) , the Native Americans there were exposed to smallpox because of contact with Europeans. As it had done elsewhere, the virus wiped out entire population groups of Native Americans. It reached Lake Ontario in 1636, and
18912-772: The Indigenous-European mixed-race mestizos (or caboclos in Brazil) of Hispanic America who, with their larger population (in most Latin American countries constituting either outright majorities, pluralities, or at the least large minorities), identify largely as a new ethnic group distinct from both Europeans and Indigenous, but still considering themselves a subset of the European-derived Hispanic or Brazilian peoplehood in culture and ethnicity ( cf. ladinos ). Among Spanish-speaking countries , indígenas or pueblos indígenas ('Indigenous peoples')
19109-630: The Malthusian assumption that "populations tend to increase to, and beyond, the limits of the food available to them at any particular level of technology." The European colonization of the Americas resulted in the deaths of so many people it contributed to climatic change and temporary global cooling , according to scientists from University College London . A century after the arrival of Christopher Columbus , some 90% of Indigenous Americans had perished from "wave after wave of disease", along with mass slavery and war, in what researchers have described as
19306-561: The Mississippi in exchange for $ 5,000,000". According to Jeffrey Ostler, "Of the 80,000 Native people who were forced west from 1830 into the 1850s, between 12,000 and 17,000 perished." Ostler states that "the large majority died of interrelated factors of starvation, exposure and disease". In addition to the removal of the Southern Tribes, there were multiple other removals of Northern Tribes also known as "Trails of Tears." For example, "In
19503-595: The Northeast, Algonquian speaking tribes in Virginia and Maryland "suffered from a variety of diseases, including malaria, typhus, and possibly smallpox." These diseases were not solely a case of Native susceptibility, however, because "as colonists took their resources, Native communities were subject to malnutrition, starvation, and social stress, all making people more vulnerable to pathogens. Repeated epidemics created additional trauma and population loss, which in turn disrupted
19700-646: The Plata basin. Colonist violence towards Indigenous peoples accelerated the loss of lives. European colonists perpetrated massacres on the Indigenous peoples and enslaved them. According to the U.S. Bureau of the Census (1894), the North American Indian Wars of the 19th century had a known death toll of about 19,000 Europeans and 30,000 Native Americans, and an estimated total death toll of 45,000 Native Americans. The first Indigenous group encountered by Columbus,
19897-401: The RNA sequence by base-pairing the messenger RNA to transfer RNA , which carries amino acids. Since there are 4 bases in 3-letter combinations, there are 64 possible codons (4 combinations). These encode the twenty standard amino acids , giving most amino acids more than one possible codon. There are also three 'stop' or 'nonsense' codons signifying the end of the coding region; these are
20094-729: The South-Asian nationality being indiano ), but for the past 10 years has been considered offensive and pejorative. Aborígene and nativo are rarely used in Brazil in Indigenous-specific contexts (e.g., aborígene is usually understood as the ethnonym for Indigenous Australians ). The Spanish and Portuguese equivalents to Indian , nevertheless, could be used to mean any hunter-gatherer or full-blooded Indigenous person, particularly to continents other than Europe or Africa—for example, indios filipinos . Indigenous peoples of
20291-587: The Spanish were aware of the spread of smallpox, they made no mention of it until 1519, a quarter century after Columbus arrived in Hispaniola. Instead he contends that enslavement in gold and silver mines was the primary reason why the Native American population of Hispaniola dropped so significantly. and that even though disease was a factor, the Native population would have rebounded the same way Europeans did following
20488-488: The TAG, TAA, and TGA codons, (UAG, UAA, and UGA on the mRNA). Cell division is essential for an organism to grow, but, when a cell divides, it must replicate the DNA in its genome so that the two daughter cells have the same genetic information as their parent. The double-stranded structure of DNA provides a simple mechanism for DNA replication . Here, the two strands are separated and then each strand's complementary DNA sequence
20685-421: The Taínos for revolting against forced labor, despite measures put in place by the encomienda , which included religious education and protection from warring tribes, eventually led to the last great Taíno rebellion (1511–1529). Following years of mistreatment, the Taínos began to adopt suicidal behaviors, with women aborting or killing their infants and men jumping from cliffs or ingesting untreated cassava ,
20882-561: The United States are commonly known as Native Americans , Indians, as well as Alaska Natives . The term "Indian" is still used in some communities and remains in use in the official names of many institutions and businesses in Indian Country . The various nations, tribes, and bands of Indigenous peoples of the Americas have differing preferences in terminology for themselves. While there are regional and generational variations in which umbrella terms are preferred for Indigenous peoples as
21079-443: The abuses in the Americas were never entirely or permanently abolished. The Spanish also employed the pre-Columbian draft system called the mita , and treated their subjects as something between slaves and serfs . Serfs stayed to work the land; slaves were exported to the mines, where large numbers of them died. In other areas the Spaniards replaced the ruling Aztecs and Incas and divided the conquered lands among themselves ruling as
21276-553: The areas that Northern tribes were removed to were already inhabited: "The areas west of the Mississippi River were home to other Indigenous nations—Osages, Kanzas, Omahas, Ioways, Otoes and Missourias. To make room for thousands of people from the East, the government dispossessed these nations of much their lands." Ostler writes that when Northern Nations were moved onto their landing 1840, "The combined population of these western nations
21473-442: The axis of the double helix once every 10.4 base pairs, but if the DNA is twisted the strands become more tightly or more loosely wound. If the DNA is twisted in the direction of the helix, this is positive supercoiling, and the bases are held more tightly together. If they are twisted in the opposite direction, this is negative supercoiling, and the bases come apart more easily. In nature, most DNA has slight negative supercoiling that
21670-407: The canonical bases plus uracil. Twin helical strands form the DNA backbone. Another double helix may be found tracing the spaces, or grooves, between the strands. These voids are adjacent to the base pairs and may provide a binding site . As the strands are not symmetrically located with respect to each other, the grooves are unequally sized. The major groove is 22 ångströms (2.2 nm) wide, while
21867-467: The case of thalidomide, a teratogen . Others such as benzo[ a ]pyrene diol epoxide and aflatoxin form DNA adducts that induce errors in replication. Nevertheless, due to their ability to inhibit DNA transcription and replication, other similar toxins are also used in chemotherapy to inhibit rapidly growing cancer cells. DNA usually occurs as linear chromosomes in eukaryotes , and circular chromosomes in prokaryotes . The set of chromosomes in
22064-512: The catastrophic impact of disease and its relationship to U.S. policy and action even when it is right before their eyes." Historian David Stannard says that by "focusing almost entirely on disease ... contemporary authors increasingly have created the impression that the eradication of those tens of millions of people was inadvertent—a sad, but both inevitable and "unintended consequence" of human migration and progress," and asserts that their destruction "was neither inadvertent nor inevitable," but
22261-581: The cell (see below) , but the major and minor grooves are always named to reflect the differences in width that would be seen if the DNA was twisted back into the ordinary B form . In a DNA double helix, each type of nucleobase on one strand bonds with just one type of nucleobase on the other strand. This is called complementary base pairing . Purines form hydrogen bonds to pyrimidines, with adenine bonding only to thymine in two hydrogen bonds, and cytosine bonding only to guanine in three hydrogen bonds. This arrangement of two nucleotides binding together across
22458-619: The chromatin structure or else by remodeling carried out by chromatin remodeling complexes (see Chromatin remodeling ). There is, further, crosstalk between DNA methylation and histone modification, so they can coordinately affect chromatin and gene expression. For one example, cytosine methylation produces 5-methylcytosine , which is important for X-inactivation of chromosomes. The average level of methylation varies between organisms—the worm Caenorhabditis elegans lacks cytosine methylation, while vertebrates have higher levels, with up to 1% of their DNA containing 5-methylcytosine. Despite
22655-539: The conditions for diseases such as smallpox, influenza, and malaria to flourish. Unlike the populations of Europe who rebounded following the Black Death , no such rebound occurred for the Indigenous populations. Similarly, historian Jeffrey Ostler at the University of Oregon has argued that population collapses in North America throughout colonization were not due mainly to lack of Native immunity to European disease. Instead, he claims that "When severe epidemics did hit, it
22852-476: The conditions found in cells, it is not a well-defined conformation but a family of related DNA conformations that occur at the high hydration levels present in cells. Their corresponding X-ray diffraction and scattering patterns are characteristic of molecular paracrystals with a significant degree of disorder. Compared to B-DNA, the A-DNA form is a wider right-handed spiral, with a shallow, wide minor groove and
23049-671: The contact and colonization period and were documented in historical accounts of the time. A few, such as the Mayan, Olmec, Mixtec, Aztec , and Nahua peoples , had their written languages and records. However, the European colonists of the time worked to eliminate non-Christian beliefs and burned many pre-Columbian written records. Only a few documents remained hidden and survived, leaving contemporary historians with glimpses of ancient culture and knowledge. According to both Indigenous and European accounts and documents, American civilizations before and at
23246-405: The creation of new genes through the process of gene duplication and divergence . A gene is a sequence of DNA that contains genetic information and can influence the phenotype of an organism. Within a gene, the sequence of bases along a DNA strand defines a messenger RNA sequence, which then defines one or more protein sequences. The relationship between the nucleotide sequences of genes and
23443-449: The cytoplasm called the nucleoid . The genetic information in a genome is held within genes, and the complete set of this information in an organism is called its genotype . A gene is a unit of heredity and is a region of DNA that influences a particular characteristic in an organism. Genes contain an open reading frame that can be transcribed, and regulatory sequences such as promoters and enhancers , which control transcription of
23640-635: The deaths of thousands of innocent people. The law calls his offense criminal negligence. Yet in light of all the deaths, the almost complete annihilation of the Mandans, and the terrible suffering the region endured, the label criminal negligence is benign, hardly befitting an action that had such horrendous consequences." However, some sources attribute the 1836–40 epidemic to the deliberate communication of smallpox to Native Americans, with historian Ann F. Ramenofsky writing, " Variola Major can be transmitted through contaminated articles such as clothing or blankets. In
23837-430: The decrease of the population correlates with the end of religious orders' control of the water. In following centuries, accusations and discussions of biological warfare were common. Well-documented accounts of incidents involving both threats and acts of deliberate infection are very rare, but may have occurred more frequently than scholars have previously acknowledged. Many of the instances likely went unreported, and it
24034-523: The depopulation of the Antilles . The practices of forced labor, brutal punishment, and inadequate necessities of life, were the initial and major reasons for depopulation. Jason Hickel estimates that a third of Arawak workers died every six months from forced labor in these mines. In this way, "slavery has emerged as a major killer" of the Indigenous populations of the Caribbean between 1492 and 1550, as it set
24231-489: The development, functioning, growth and reproduction of all known organisms and many viruses . DNA and ribonucleic acid (RNA) are nucleic acids . Alongside proteins , lipids and complex carbohydrates ( polysaccharides ), nucleic acids are one of the four major types of macromolecules that are essential for all known forms of life . The two DNA strands are known as polynucleotides as they are composed of simpler monomeric units called nucleotides . Each nucleotide
24428-498: The dominant Hispanic culture . In recent years, the self-identified Indigenous population in many countries has increased as individuals reclaim their heritage amid rising Indigenous-led movements for self-determination and social justice . Application of the term " Indian " originated with Christopher Columbus , who, in his search for India , thought that he had arrived in the East Indies . The islands came to be known as
24625-405: The double helix (from six-carbon ring to six-carbon ring) is called a Watson-Crick base pair. DNA with high GC-content is more stable than DNA with low GC -content. A Hoogsteen base pair (hydrogen bonding the 6-carbon ring to the 5-carbon ring) is a rare variation of base-pairing. As hydrogen bonds are not covalent , they can be broken and rejoined relatively easily. The two strands of DNA in
24822-442: The edges of the bases and chelation of a metal ion in the centre of each four-base unit. Other structures can also be formed, with the central set of four bases coming from either a single strand folded around the bases, or several different parallel strands, each contributing one base to the central structure. In addition to these stacked structures, telomeres also form large loop structures called telomere loops, or T-loops. Here,
25019-481: The end of an otherwise complementary double-strand of DNA. However, branched DNA can occur if a third strand of DNA is introduced and contains adjoining regions able to hybridize with the frayed regions of the pre-existing double-strand. Although the simplest example of branched DNA involves only three strands of DNA, complexes involving additional strands and multiple branches are also possible. Branched DNA can be used in nanotechnology to construct geometric shapes, see
25216-496: The end of the fifteenth century, they spread throughout the southern and northern hemispheres, leaving the Indigenous populations in near ruins. No evidence has been discovered that the earliest Spanish colonists and missionaries deliberately attempted to infect the American Natives, and some efforts were made to limit the devastating effects of disease before it killed off what remained of their labor force (compelled to work under
25413-519: The extermination of Indigenous peoples took the lives of 100 million people: "...the total extermination of many American Indian peoples and the near-extermination of others, in numbers that eventually totaled close to 100,000,000." A 2019 study estimates the pre-Columbian Indigenous population contained more than 60 million people, but dropped to 6 million by 1600, based on a drop in atmospheric CO 2 during that period. Other studies have disputed this conclusion. The Indigenous population of
25610-626: The first centuries of European colonization. Most scholars estimate a pre-colonization population of around 50 million, with other scholars arguing for an estimate of 100 million. Estimates reach as high as 145 million. Epidemics ravaged the Americas with diseases, such as smallpox , measles , and cholera , which the early colonists brought from Europe. The spread of infectious diseases was slow initially, as most Europeans were not actively or visibly infected, due to inherited immunity from generations of exposure to these diseases in Europe. This changed when
25807-418: The focus is on the interactions between DNA and other molecules that mediate the function of the genome. Genomic DNA is tightly and orderly packed in the process called DNA condensation , to fit the small available volumes of the cell. In eukaryotes, DNA is located in the cell nucleus , with small amounts in mitochondria and chloroplasts . In prokaryotes, the DNA is held within an irregularly shaped body in
26004-579: The following: Some Spaniards objected to the encomienda system of labor, notably Bartolomé de las Casas , who insisted that the Indigenous people were humans with souls and rights. Because of many revolts and military encounters, Emperor Charles V helped relieve the strain on both the Native laborers and the Spanish vanguards probing the Caribana for military and diplomatic purposes. Later on New Laws were promulgated in Spain in 1542 to protect isolated Native, but
26201-502: The fragmentary nature of the evidence. Estimates range from 8–112 million. Scholars have varied widely on the estimated size of the Indigenous populations prior to colonization and on the effects of European contact . Estimates are made by extrapolations from small bits of data. In 1976, geographer William Denevan used the existing estimates to derive a "consensus count" of about 54 million people. Nonetheless, more recent estimates still range widely. In 1992, Denevan suggested that
26398-656: The free labor states of the North, federal and state officials, supported by farmers, speculators and business interests, evicted Shawnees, Delawares, Senecas, Potawatomis, Miamis, Wyandots, Ho-Chunks, Ojibwes, Sauks and Meskwakis." These Nations were moved West of the Mississippi into what is now known as Eastern Kansas, and numbered 17,000 on arrival. According to Ostler, "by 1860, their numbers had been cut in half" because of low fertility, high infant mortality, and increased disease caused by conditions such as polluted drinking water, few resources, and social stress. Ostler also writes that
26595-448: The functions of these RNAs are not entirely clear. One proposal is that antisense RNAs are involved in regulating gene expression through RNA-RNA base pairing. A few DNA sequences in prokaryotes and eukaryotes, and more in plasmids and viruses , blur the distinction between sense and antisense strands by having overlapping genes . In these cases, some DNA sequences do double duty, encoding one protein when read along one strand, and
26792-474: The general disruption of societies. Scholarly disputes remain over the degree to which each factor contributed or should be emphasized; some modern scholars have categorized it as a genocide , claiming that deliberate, systematic actions by Europeans were the primary cause. Traditional scholars have disputed this characterization, maintaining that incidental disease exposure was the primary cause. Pre-Columbian population figures are difficult to estimate because of
26989-571: The growth of herbs and berry-producing plants, which were important both for food and for medicines. In the Mississippi River valley, Europeans noted that Native Americans managed groves of nut and fruit trees not far from villages and towns and their gardens and agricultural fields. They would have used prescribed burning farther away, in forest and prairie areas. Many crops first domesticated by Indigenous peoples are now produced and used globally, most notably maize (or "corn") arguably
27186-435: The health outcomes of Indigenous peoples. The study defined negative health outcomes to include such concepts as anxiety , suicidal ideation , suicide attempts , polysubstance abuse , PTSD , depression , binge eating , anger, and sexual abuse. The connection between IHT and health conditions is complicated because of the difficult nature of measuring IHT, the unknown directionality of IHT and health outcomes, and because
27383-448: The helical axis in a left-handed spiral, the opposite of the more common B form. These unusual structures can be recognized by specific Z-DNA binding proteins and may be involved in the regulation of transcription. For many years, exobiologists have proposed the existence of a shadow biosphere , a postulated microbial biosphere of Earth that uses radically different biochemical and molecular processes than currently known life. One of
27580-448: The hydration level, DNA sequence, the amount and direction of supercoiling, chemical modifications of the bases, the type and concentration of metal ions , and the presence of polyamines in solution. The first published reports of A-DNA X-ray diffraction patterns —and also B-DNA—used analyses based on Patterson functions that provided only a limited amount of structural information for oriented fibers of DNA. An alternative analysis
27777-427: The hydrolytic activities of cellular water, etc., also occur frequently. Although most of these damages are repaired, in any cell some DNA damage may remain despite the action of repair processes. These remaining DNA damages accumulate with age in mammalian postmitotic tissues. This accumulation appears to be an important underlying cause of aging. Many mutagens fit into the space between two adjacent base pairs, this
27974-412: The importance of 5-methylcytosine, it can deaminate to leave a thymine base, so methylated cytosines are particularly prone to mutations . Other base modifications include adenine methylation in bacteria, the presence of 5-hydroxymethylcytosine in the brain , and the glycosylation of uracil to produce the "J-base" in kinetoplastids . DNA can be damaged by many sorts of mutagens , which change
28171-557: The imposition and restriction of Indigenous peoples and cultures by the Canadian Government. The terms "Native" and " Eskimo " are generally regarded as disrespectful (in Canada), and so are rarely used unless specifically required. While "Indigenous peoples" is the preferred term, many individuals or communities may choose to describe their identity using a different term. The Métis people of Canada can be contrasted, for instance, to
28368-684: The lands of the Iroquois by 1679. During the 1770s smallpox killed at least 30% of the West Coast Native Americans. The 1775–82 North American smallpox epidemic and the 1837 Great Plains smallpox epidemic brought devastation and drastic population depletion among the Plains Indians . In 1832 the federal government of the United States established a smallpox vaccination program for Native Americans ( The Indian Vaccination Act of 1832 ). The Indigenous peoples in Brazil declined from
28565-490: The last three decades of the 16th century, the population of present-day Mexico dropped to about one million people. The Maya population is today estimated at six million, which is about the same as at the end of the 15th century, according to some estimates. In what is now Brazil, the Indigenous population declined from a pre-Columbian high of an estimated four million to some 300,000. Over 60 million Brazilians possess at least one Native South American ancestor, according to
28762-561: The link between physical colonial violence such as warfare, displacement, and enslavement, and the proliferation of disease among Native populations. For example, according to Coquille scholar Dina Gilio-Whitaker , "In recent decades, however, researchers challenge the idea that disease is solely responsible for the rapid Indigenous population decline. The research identifies other aspects of European contact that had profoundly negative impacts on Native peoples' ability to survive foreign invasion: war, massacres, enslavement, overwork, deportation,
28959-514: The loss of will to live or reproduce, malnutrition and starvation from the breakdown of trade networks, and the loss of subsistence food production due to land loss." Further, Andrés Reséndez of the University of California, Davis points out that, even though the Spanish were aware of deadly diseases such as smallpox, there is no mention of them in the New World until 1519, implying that, until that date, epidemic disease played no significant part in
29156-476: The methodologies to measure IHT include a "Historical Losses Scale" (HLS), "Historical Losses Associated Symptoms Scale" (HLASS), and residential school ancestry studies. HLS uses a survey format that includes "12 kinds of historical losses", such as loss of language and loss of land and asks participants how often they think about those losses. The HLASS includes 12 emotional reactions, and asks participants how they feel when they think about these losses. Lastly,
29353-441: The minor groove is 12 Å (1.2 nm) in width. Due to the larger width of the major groove, the edges of the bases are more accessible in the major groove than in the minor groove. As a result, proteins such as transcription factors that can bind to specific sequences in double-stranded DNA usually make contact with the sides of the bases exposed in the major groove. This situation varies in unusual conformations of DNA within
29550-516: The mitochondrial genome (constituting up to 90% of the DNA of the cell). A DNA sequence is called a "sense" sequence if it is the same as that of a messenger RNA copy that is translated into protein. The sequence on the opposite strand is called the "antisense" sequence. Both sense and antisense sequences can exist on different parts of the same strand of DNA (i.e. both strands can contain both sense and antisense sequences). In both prokaryotes and eukaryotes, antisense RNA sequences are produced, but
29747-477: The most dangerous are double-strand breaks, as these are difficult to repair and can produce point mutations , insertions , deletions from the DNA sequence, and chromosomal translocations . These mutations can cause cancer . Because of inherent limits in the DNA repair mechanisms, if humans lived long enough, they would all eventually develop cancer. DNA damages that are naturally occurring , due to normal cellular processes that produce reactive oxygen species,
29944-882: The most important crop in the world. Other significant crops include cassava ; chia ; squash (pumpkins, zucchini, marrow , acorn squash , butternut squash ); the pinto bean , Phaseolus beans including most common beans , tepary beans , and lima beans ; tomatoes ; potatoes ; sweet potatoes ; avocados ; peanuts ; cocoa beans (used to make chocolate ); vanilla ; strawberries ; pineapples ; peppers (species and varieties of Capsicum , including bell peppers , jalapeños , paprika , and chili peppers ); sunflower seeds ; rubber ; brazilwood ; chicle ; tobacco ; coca ; blueberries , cranberries , and some species of cotton . Studies of contemporary Indigenous environmental management—including agro-forestry practices among Itza Maya in Guatemala and hunting and fishing among
30141-410: The nation. As may be expected among people of over 400 different cultures in the US alone, not all of the people intended to be described by this term have agreed on its use or adopted it. No single group naming convention has been accepted by all Indigenous peoples in the Americas. Most prefer to be addressed as people of their tribe or nations when not speaking about Native Americans/American Indians as
30338-503: The new feudal lords with often, but unsuccessful lobbying to the viceroys of the Spanish crown to pay Tlaxcalan war demnities. The infamous Bandeirantes from São Paulo , adventurers mostly of mixed Portuguese and Native ancestry, penetrated steadily westward in their search for Indian slaves . Serfdom existed as such in parts of Latin America well into the 19th century, past independence. Historian Andrés Reséndez argues that even though
30535-464: The new strand, and the cell ends up with a perfect copy of its DNA. Naked extracellular DNA (eDNA), most of it released by cell death, is nearly ubiquitous in the environment. Its concentration in soil may be as high as 2 μg/L, and its concentration in natural aquatic environments may be as high at 88 μg/L. Various possible functions have been proposed for eDNA: it may be involved in horizontal gene transfer ; it may provide nutrients; and it may act as
30732-473: The nineteenth century, the U. S. Army sent contaminated blankets to Native Americans, especially Plains groups, to control the Indian problem." In Brazil, well into the 20th century, deliberate infection attacks continued as Brazilian settlers and miners transported infections intentionally to the Native groups whose lands they coveted. After Edward Jenner 's 1796 demonstration that the smallpox vaccination worked,
30929-454: The open reading frame. In many species , only a small fraction of the total sequence of the genome encodes protein. For example, only about 1.5% of the human genome consists of protein-coding exons , with over 50% of human DNA consisting of non-coding repetitive sequences . The reasons for the presence of so much noncoding DNA in eukaryotic genomes and the extraordinary differences in genome size , or C-value , among species, represent
31126-452: The operation so earnestly they decide that it must be some new mode or trick of the pale face by which they hope to gain some new advantage over them." So great was the distrust of the settlers that the Mandan chief Four Bears denounced the white man, whom he had previously treated as brothers, for deliberately bringing the disease to his people. During the siege of British-held Fort Pitt in
31323-428: The place of thymine in RNA and differs from thymine by lacking a methyl group on its ring. In addition to RNA and DNA, many artificial nucleic acid analogues have been created to study the properties of nucleic acids, or for use in biotechnology. Modified bases occur in DNA. The first of these recognized was 5-methylcytosine , which was found in the genome of Mycobacterium tuberculosis in 1925. The reason for
31520-530: The presence of these noncanonical bases in bacterial viruses ( bacteriophages ) is to avoid the restriction enzymes present in bacteria. This enzyme system acts at least in part as a molecular immune system protecting bacteria from infection by viruses. Modifications of the bases cytosine and adenine, the more common and modified DNA bases, play vital roles in the epigenetic control of gene expression in plants and animals. A number of noncanonical bases are known to occur in DNA. Most of these are modifications of
31717-420: The previous 100 years. These wars cost the lives of approximately 19,000 white people, and the lives of about 30,000 Indians, including men, women, and children. They safely estimated that the number of Native people who were killed or wounded was actually around fifty percent more than what was recorded. There is some disagreement among scholars about how widespread warfare was in pre-Columbian America, but there
31914-412: The prime symbol being used to distinguish these carbon atoms from those of the base to which the deoxyribose forms a glycosidic bond . Therefore, any DNA strand normally has one end at which there is a phosphate group attached to the 5′ carbon of a ribose (the 5′ phosphoryl) and another end at which there is a free hydroxyl group attached to the 3′ carbon of a ribose (the 3′ hydroxyl). The orientation of
32111-466: The proposals was the existence of lifeforms that use arsenic instead of phosphorus in DNA . A report in 2010 of the possibility in the bacterium GFAJ-1 was announced, though the research was disputed, and evidence suggests the bacterium actively prevents the incorporation of arsenic into the DNA backbone and other biomolecules. At the ends of the linear chromosomes are specialized regions of DNA called telomeres . The main function of these regions
32308-444: The provision of healthcare." Such conditions would continue, alongside rampant disease in Native communities, throughout colonization, the formation of the United States, and multiple forced removals, as Ostler explains that many scholars "have yet to come to grips with how U.S. expansion created conditions that made Native communities acutely vulnerable to pathogens and how severely disease impacted them. ... Historians continue to ignore
32505-497: The repeated and forced removal from their land. Beginning in the 1830s, there was the relocation of an estimated 100,000 Indigenous people in the United States called the " Trail of Tears ". The tribes affected by this specific removal were the Five Civilized Tribes : The Cherokee, Creek, Chickasaw, Choctaw, and Seminole. The treaty of New Echota, was enacted, which stated that the United States "would give Cherokee land west of
32702-705: The rest of their lives. But contact with adult populations without this childhood or inherited immunity would result in these diseases proving fatal. Colonization of the Caribbean led to the destruction of the Arawaks of the Lesser Antilles . Their culture was destroyed by 1650. Only 500 had survived by the year 1550, though the bloodlines continued through to the modern populace. In Amazonia, Indigenous societies weathered, and continue to suffer, centuries of colonization and genocide. Contact with European diseases such as smallpox and measles killed between 50 and 67 percent of
32899-477: The result of arbitrary formulas applied from unreliable sources. Population size for Native American tribes is very difficult to state definitively, but at least one writer has made estimates, often based on an assumed proportion of the number of warriors to total population for the tribe. Typical proportions were 5 people per one warrior and at least 1 up to 5 warriors (therefore at least 5–25 people) per lodge, cabin or house. The total peak population size only for
33096-638: The result of microbial pestilence and purposeful genocide working in tandem. He also wrote: ...Despite frequent undocumented assertions that disease was responsible for the great majority of indigenous deaths in the Americas, there does not exist a single scholarly work that even pretends to demonstrate this claim on the basis of solid evidence. And that is because there is no such evidence, anywhere. The supposed truism that more native people died from disease than from direct face-to-face killing or from gross mistreatment or other concomitant derivatives of that brutality such as starvation, exposure, exhaustion, or despair
33293-409: The same biological information . This information is replicated when the two strands separate. A large part of DNA (more than 98% for humans) is non-coding , meaning that these sections do not serve as patterns for protein sequences . The two strands of DNA run in opposite directions to each other and are thus antiparallel . Attached to each sugar is one of four types of nucleobases (or bases ). It
33490-540: The same time as that of Egypt . Many later pre-Columbian civilizations achieved great complexity, with hallmarks that included permanent or urban settlements, agriculture, engineering, astronomy, trade, civic and monumental architecture, and complex societal hierarchies . Some of these civilizations had long faded by the time of the first significant European and African arrivals (ca. late 15th–early 16th centuries), and are known only through oral history and through archaeological investigations. Others were contemporary with
33687-432: The section on uses in technology below. Several artificial nucleobases have been synthesized, and successfully incorporated in the eight-base DNA analogue named Hachimoji DNA . Dubbed S, B, P, and Z, these artificial bases are capable of bonding with each other in a predictable way (S–B and P–Z), maintain the double helix structure of DNA, and be transcribed to RNA. Their existence could be seen as an indication that there
33884-473: The shape of a double helix . The nucleotide contains both a segment of the backbone of the molecule (which holds the chain together) and a nucleobase (which interacts with the other DNA strand in the helix). A nucleobase linked to a sugar is called a nucleoside , and a base linked to a sugar and to one or more phosphate groups is called a nucleotide . A biopolymer comprising multiple linked nucleotides (as in DNA)
34081-498: The single-stranded DNA curls around in a long circle stabilized by telomere-binding proteins. At the very end of the T-loop, the single-stranded telomere DNA is held onto a region of double-stranded DNA by the telomere strand disrupting the double-helical DNA and base pairing to one of the two strands. This triple-stranded structure is called a displacement loop or D-loop . In DNA, fraying occurs when non-complementary regions exist at
34278-502: The small pox among the disaffected tribes of Indians? We must on this occasion use every stratagem in our power to reduce them." His Colonel agreed to try. Most scholars have asserted that the 1837 Great Plains smallpox epidemic was "started among the tribes of the upper Missouri River by failure to quarantine steamboats on the river", and Captain Pratt of the St. Peter "was guilty of contributing to
34475-563: The smallpox out of a bottle and destroy them." The Reverend Isaac McCoy was quoted in his History of Baptist Indian Missions as saying that the white men had deliberately spread smallpox among the Indians of the southwest, including the Pawnee tribe, and the havoc it made was reported to General Clark and the Secretary of War. Artist and writer George Catlin observed that Native Americans were also suspicious of vaccination, "They see white men urging
34672-518: The strands separate and exist in solution as two entirely independent molecules. These single-stranded DNA molecules have no single common shape, but some conformations are more stable than others. In humans, the total female diploid nuclear genome per cell extends for 6.37 Gigabase pairs (Gbp), is 208.23 cm long and weighs 6.51 picograms (pg). Male values are 6.27 Gbp, 205.00 cm, 6.41 pg. Each DNA polymer can contain hundreds of millions of nucleotides, such as in chromosome 1 . Chromosome 1
34869-469: The sugar-phosphate to form the complete nucleotide, as shown for adenosine monophosphate . Adenine pairs with thymine and guanine pairs with cytosine, forming A-T and G-C base pairs . The nucleobases are classified into two types: the purines , A and G , which are fused five- and six-membered heterocyclic compounds , and the pyrimidines , the six-membered rings C and T . A fifth pyrimidine nucleobase, uracil ( U ), usually takes
35066-459: The technique became better known and smallpox became less deadly in the United States and elsewhere. Many colonists and Natives were vaccinated, although, in some cases, officials tried to vaccinate Natives only to discover that the disease was too widespread to stop. At other times, trade demands led to broken quarantines. In other cases, Natives refused vaccination because of suspicion of whites. The first international healthcare expedition in history
35263-474: The term Indigenous people used in the various samples comprises a huge population of individuals with drastically different experiences and histories. That being said some studies such as Bombay, Matheson, and Anisman (2014), Elias et al. (2012), and Pearce et al. (2008) found that Indigenous respondents with a connection to residential schools have more negative health outcomes (e.g., suicide ideation, suicide attempts, and depression) than those who did not have
35460-497: The term because Indigenous cultures existed before European colonization. Indigenous groups have territorial claims that are different from modern national and international borders, and when labeled as part of a country, their traditional lands are not acknowledged. Some who have written guidelines consider it more appropriate to describe an Indigenous person as "living in" or "of" the Americas, rather than calling them "American"; or simply calling them "Indigenous" without any addition of
35657-565: The term usually includes the history of Indigenous cultures until Europeans either conquered or significantly influenced them. "Pre-Columbian" is used especially often in the context of discussing the pre-contact Mesoamerican Indigenous societies : Olmec ; Toltec ; Teotihuacano ' Zapotec ; Mixtec ; Aztec and Maya civilizations ; and the complex cultures of the Andes : Inca Empire , Moche culture , Muisca Confederation , and Cañari . The Pre-Columbian era refers to all period subdivisions in
35854-552: The threats were carried out, or how effective individual attempts were, is uncertain. One such threat was delivered by fur trader James McDougall , who is quoted as saying to a gathering of local chiefs, "You know the smallpox. Listen: I am the smallpox chief. In this bottle I have it confined. All I have to do is to pull the cork, send it forth among you, and you are dead men. But this is for my enemies and not my friends." Likewise, another fur trader threatened Pawnee Indians that if they didn't agree to certain conditions, "he would let
36051-454: The time of European encounter had achieved great complexity and many accomplishments. For instance, the Aztecs built one of the largest cities in the world, Tenochtitlan (the historical site of what would become Mexico City ), with an estimated population of 200,000 for the city proper and a population of close to five million for the extended empire. By comparison, the largest European cities in
36248-518: The total population was approximately 53.9 million and the populations by region were, approximately, 3.8 million for the United States and Canada, 17.2 million for Mexico, 5.6 million for Central America, 3 million for the Caribbean, 15.7 million for the Andes and 8.6 million for lowland South America. A 2020 genetic study suggests that prior estimates for the pre-Columbian Caribbean population may have been at least tenfold too large. Historian David Stannard estimates that
36445-514: The tribes listed in this table is 3,529,240 in the US and Canada (including 507,675 in Canada). This number is very similar to Snow's estimate for the US and Canada and to Alchon's, Denevan's and Milner's estimates. Genetic diversity and population structure in the American land mass using DNA micro-satellite markers ( genotype ) sampled from North, Central, and South America have been analyzed against similar data available from other Indigenous populations worldwide. The Amerindian populations show
36642-430: The two separate polynucleotide strands are bound together, according to base pairing rules (A with T and C with G), with hydrogen bonds to make double-stranded DNA. The complementary nitrogenous bases are divided into two groups, the single-ringed pyrimidines and the double-ringed purines . In DNA, the pyrimidines are thymine and cytosine; the purines are adenine and guanine. Both strands of double-stranded DNA store
36839-583: The war and the colonization hampered the traditional economy, and possibly led to shortages of food and materials. Across the western hemisphere, war with various Native American civilizations constituted alliances based out of both necessity or economic prosperity and, resulted in mass-scale intertribal warfare. European colonization in the North American continent also contributed to a number of wars between Native Americans, who fought over which of them should have first access to new technology and weaponry—like in
37036-456: Was 145 million in the late 15th and by the late 17th century, had been reduced to 15 million due to epidemics , wars, massacres, mass rapes , starvation, and enslavement. Indigenous historical trauma (IHT) is the trauma that can accumulate across generations and develop as a result of the historical ramifications of colonization and is linked to mental and physical health hardships and population decline. IHT affects many different people in
37233-584: Was 9,000 ... 20 years later, it had fallen to 6,000." On 8 September 2000, the head of the United States Bureau of Indian Affairs (BIA) formally apologized for the agency's participation in the ethnic cleansing of Western tribes. In a speech before representatives of Native American peoples in June 2019, California governor Gavin Newsom apologized for the " California Genocide ." Newsom said, "That's what it was,
37430-583: Was cultivated as long as 10,000 years ago. According to Linda Newson , "It is clear that in pre-Columbian times some groups struggled to survive and often suffered food shortages and famines , while others enjoyed a varied and substantial diet." Persistent drought around AD 850 coincided with the collapse of the Classic Maya civilization, and the famine of One Rabbit (AD 1454) was a major catastrophe in Mexico. Indigenous peoples of North America began practicing farming approximately 4,000 years ago, late in
37627-635: Was immediately rejected by some leading members of the Association, and, while adopted by many, it was never universally accepted. While never popular in Indigenous communities themselves, it remains a preferred term among some anthropologists, notably in some parts of Canada and the English-speaking Caribbean . " Indigenous peoples in Canada " is used as the collective name for First Nations , Inuit , and Métis . The term Aboriginal peoples as
37824-497: Was not originally accepted by the myriad groups of Indigenous peoples themselves but has since been embraced or tolerated by many over the last two centuries. The term "Indian" generally does not include the culturally and linguistically distinct Indigenous peoples of the Arctic regions of the Americas, including the Aleuts , Inuit , or Yupik peoples . These peoples entered the continent as
38021-494: Was often less because Native bodies lacked immunity than because European colonialism disrupted Native communities and damaged their resources, making them more vulnerable to pathogens." In specific regard to Spanish colonization of northern Florida and southeastern Georgia, Native peoples there "were subject to forced labor and, because of poor living conditions and malnutrition, succumbed to wave after wave of unidentifiable diseases." Further, in relation to British colonization in
38218-517: Was proposed by Wilkins et al. in 1953 for the in vivo B-DNA X-ray diffraction-scattering patterns of highly hydrated DNA fibers in terms of squares of Bessel functions . In the same journal, James Watson and Francis Crick presented their molecular modeling analysis of the DNA X-ray diffraction patterns to suggest that the structure was a double helix. Although the B-DNA form is most common under
38415-741: Was the Balmis Expedition which had the aim of vaccinating Indigenous peoples against smallpox all along the Spanish Empire in 1803. In 1831, government officials vaccinated the Yankton Dakota at Sioux Agency . The Santee Sioux refused vaccination and many died. While epidemic disease was a leading factor of the population decline of the American Indigenous peoples after 1492, there were other contributing factors, all of them related to European contact and colonization. One of these factors
38612-409: Was the overwhelming cause of the population decline of the Indigenous peoples. After initial contact with Europeans and Africans, Old World diseases caused the deaths of 90 to 95% of the native population of the New World in the following 150 years. Smallpox killed from one-third to half of the native population of Hispaniola in 1518. By killing the Incan ruler Huayna Capac , smallpox caused
38809-423: Was warfare. According to demographer Russell Thornton, although many people died in wars over the centuries, and war sometimes contributed to the near extinction of certain tribes, warfare and death by other violent means was a comparatively minor cause of overall Native population decline. From the U.S. Bureau of the Census in 1894, wars between the government and the Indigenous peoples ranged over 40 in number over
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