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42-578: The Frozen Ark is a charitable frozen zoo project created jointly by the Zoological Society of London , the Natural History Museum and University of Nottingham . The project aims to preserve the DNA and living cells of endangered species to retain the genetic knowledge for the future. The Frozen Ark collects and stores samples taken from animals in zoos and those threatened with extinction in

84-568: A geneticist who developed the idea of cryopreserving genetic material from species considered to be endangered. His ideas led to the creation of the Frozen Zoo as a genetic library. There is a breeding herd in the San Diego Zoo Safari Park . Once the foal matures, he will be relocated to the breeding herd at the San Diego Zoo Safari Park , so as to pass Kuporovic's genes into the larger captive Przewalski's horse population and increase

126-448: A golden pheasant and five red jungle fowl . Others soon followed, including muntjac deer , llamas , wombats and skunks . In 1931 Whipsnade Park was opened to the public as the world's first open zoological park. In 1960–61, Lord Zuckerman , then Secretary of ZSL, raised funds from two medical foundations to found laboratories as an Institute of Zoology where scientists would be employed by ZSL and undertake research. The Society

168-687: A breeding Pyrenean ibex population. The Frozen Zoo at the San Diego Zoo's Institute for Conservation Research currently stores a collection of 8,400 samples from over 800 species and subspecies. It has acted as a forebear to similar projects at other zoos in the United States and Europe. However, there are still less than a dozen frozen zoos worldwide. At the United Arab Emirates' Breeding Centre for Endangered Arabian Wildlife (BCEAW) in Sharjah ,

210-491: A breeding population. Despite the death of the ibex, DNA analysis revealed that the offspring was a legitimate clone from its last living descendent. Over the years, concerns over population declines of the northern white rhinoceros ( Ceratotherium simum cottoni ) have increased with the increasing value of their horns to poachers. Specifically, the population has declined nearly seventy percent from 2011 to 2019. Processes like SCNT can help aid in conservation efforts towards

252-465: A nucleus collected from a cultured Przewalski's horse fibroblast. The resulting embryo was induced to begin division and was cultured until it reached the blastocyst stage, then implanted into a domestic horse surrogate mare, which carried the embryo to term and delivered a foal with the Przewalski's horse DNA of the long-deceased stallion. The cloned horse was named Kurt, after Dr. Kurt Benirschke ,

294-575: A zoological collection "which should interest and amuse the public." The society was founded in April 1826 by Sir Stamford Raffles , the Marquess of Lansdowne , Lord Auckland , Sir Humphry Davy , Robert Peel , Joseph Sabine , Nicholas Aylward Vigors along with various other nobility, clergy, and naturalists. Raffles was the first chairman and president, but died after only a few months in office, in July 1826. He

336-650: Is a registered charity under English law. Under the Crown Estate Act 1961 , the ZSL had a maximum lease length of 60 years from the Crown Estate , but this was changed to 150 years by the Zoological Society of London (Leases) Act 2024 (c. 20). The Institute of Zoology is the scientific research division of the ZSL. It is a government-funded research institute, which specialises in scientific issues relevant to

378-481: Is building a frozen zoo. RBC Director Steven Stice and animal and dairy science assistant professor Franklin West created the facility with the thought of saving endangered cat species. The scientists have already extracted cells from a Sumatran tiger , which could be used for artificial insemination. Artificial insemination provides a remedy for animals who, due to anatomical or physiological reasons, are unable to reproduce in

420-542: Is the most difficult to combat because of the highly reactive components of ROS, but some measures like adding certain proteins to limit freeze-thaw damage and increase the survival rate of the DNA. A gaur that died of natural causes had some skin cells frozen and added to the San Diego Frozen Zoo. Eight years later, DNA from these cells was inserted into a domestic-cow egg to create an embryo (trans-species cloning), which

462-454: Is theoretically possible to make sperm and egg cells from these IPS cells. Several animals whose cells were preserved in frozen zoos have been cloned to increase the genetic diversity of endangered species, as of 2021 . One attempt to clone an extinct species was made in 2003; the newborn Pyrenean ibex died of a development disorder which may have been linked to the cloning, and there are not enough genetic samples in frozen zoos to re-create

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504-494: Is usually low, can be increased through hormone treatment to obtain 10–20 oocytes , dependent on the species. Some frozen zoos prefer to fertilize eggs and freeze the resulting embryo, as embryos are more resilient under the cryopreservation process. Some centers also collect skin cell samples of endangered animals or extinct species. The Scripps Research Institute has successfully made skin cells into cultures of special cells called induced pluripotent stem cells ( IPS cells ). It

546-592: The International Zoo Yearbook and Remote Sensing in Ecology and Conservation . The society administers the following award programmes: Individuals can be elected Fellows of the Zoological Society of London and therefore granted the post-nominal letters FZS . Living Honorary ZSL Fellows ( Hon. FZS ) comprise: The council is the governing body of the ZSL. There are 15 council members, led by

588-441: The 1980s had already substantially increased the genetic diversity of the captive population, after he was discovered to have more unique alleles than any other horse living at the time, including otherwise-lost genetic material from two of the original captive founders. To produce the clone, frozen skin fibroblasts were thawed, and grown in cell culture . An oocyte was collected from a domestic horse, and its nucleus replaced by

630-571: The Audubon Center for Research of Endangered Species cryopreserve genetic material in order to protect the diversity of the gene pool of endangered species, or to provide for a prospective reintroduction of such extinct species as the Tasmanian tiger and the mammoth . Gathering material for a frozen zoo is rendered simple by the abundance of sperm in males. Sperm can be taken from an animal following death. The production of eggs, which in females

672-557: The Southern White Rhinoceros ( Ceratotherium simum simum ). Frozen-thawed semen has been tested and has seen some successes, helping solve issues with reproduction of the species as a whole. Zoological Society of London The Zoological Society of London ( ZSL ) is a charity devoted to the worldwide conservation of animals and their habitats . It was founded in 1826. Since 1828, it has maintained London Zoo , and since 1931 Whipsnade Zoo . On 29 November 1822,

714-536: The Zoological Gardens were opened to members. In 1831 William IV presented the Royal Menagerie to the Zoological Society, and in 1847 the public was admitted to aid funding, and Londoners soon christened the Zoological Gardens the " Zoo ". London Zoo soon had the most extensive collection of animals in the world. A History of the ZSL , written by Henry Scherren (FZS), was published in 1905. The History

756-697: The birthday of John Ray , "the father of modern zoology", a meeting held in the Linnean Society in Soho Square led by Rev. William Kirby , resolved to form a "Zoological Club of the Linnean Society of London". Between 1816 and 1826, discussions between Stamford Raffles, Humphry Davy, Joseph Banks and others led to the idea that London should have an establishment similar to the Jardin des Plantes in Paris. It would house

798-547: The conservation of species and their habitats. The Institute of Zoology focuses its research on five areas: evolutionary biology , genetics , ecology , reproductive biology and wildlife epidemiology. The Institute of Zoology was graded 4 in the 1997–2001 UK Research Assessment Exercise, and publishes reports annually. From the late 1980s the Institute of Zoology had been affiliated to the University of London . However, in 2000 this

840-513: The database. In the DNA laboratory, samples are contained after collection from scientists, and different research projects are conducted there. The consortium acts as a bridge to bring together different, but important, groups from zoos, aquariums, museums, and universities. The taxon expert groups monitor the major phyla and lists like the IUCN Red List. The database is the essential piece as it holds all reports and records needed to perform all of

882-526: The embryos stored include the extremely endangered Gordon's wildcat ( Felis silvestris gordoni ) and the Arabian leopard ( Panthera pardus nimr ) (of which there are only 50 in the wild). The Audubon Center for Research of Endangered Species, affiliated with the University of New Orleans , is maintaining a frozen zoo. In 2000 the Center implanted a frozen-thawed embryo from the highly endangered African wildcat into

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924-582: The first clone to survive beyond infancy. Scientists at Advanced Cell Technology in Worcester, Massachusetts , extracted DNA from skin cells of a dead male banteng, that were preserved in San Diego 's Frozen Zoo facility, and transferred it into eggs from domestic banteng cows, a process called somatic cell nuclear transfer . Thirty embryos were created and implanted in domestic banteng cows. Two were carried to term and delivered by Caesarian section . The first

966-464: The freezing process, causing differing osmotic pressures within the cell. Oxidative stress is the result of too many reactive oxygen species (ROS), which is highly reactive and damaging to all parts of the cell. Although these stressors are present within the cell, there are solutions to each. By introducing cholesterol to the samples, temperature shock can be reduced. The use of antifreeze proteins provides one solution for osmotic stress. Oxidative stress

1008-555: The genetic variation of the species. To help mitigate inbreeding depression for two endangered species, the Black-footed ferret (Mustela nigripes) , Revive & Restore facilitates on-going efforts to clone individuals from historic cell lines stored at the San Diego Zoo Wildlife Alliance Frozen Zoo. The program seeks to restore genetic variation lost from the living gene pool. On December 10, 2020,

1050-410: The natural way. Reproduction of stored genetic material also allows for the fostering of genetic improvements, and the prevention of inbreeding. Modern technology allows for genetic manipulation in animals without keeping them in captivity. However, the success of their restoration into the wild would require the application of new science and a sufficient amount of previously collected material. Due to

1092-461: The non-cloned population. The Pyrenean ibex went extinct in 2000. In 2003 frozen cells from the last one (a female killed by a falling branch) were used to clone 208 embryos, of which 7 successfully implanted in goats, and one made it to term. That one ibex died of respiratory failure just after birth; quite possibly as a result of the cloning process, its lungs had not developed properly. There may not be enough individuals' cells preserved to create

1134-454: The other functions for the charity. The hope for the future is for zoos and aquariums to be able to collect samples from their threatened and/or endangered species in house to help with conservation efforts. The collection and freezing of these samples allows for the distribution of gametes among populations. Samples can be collected from living hosts and from deceased hosts as well. The University of Georgia 's Regenerative Bioscience Center

1176-475: The president and served by the secretary and treasurer. Council members are the trustees of the society and serve for up to five years at a time. The Presidency is a voluntary position, with the role of leading the ZSL Council. The Society's Presidents and their dates in office are: The post of secretary is honorary and under the society's constitution carries the responsibility for the day-to-day management of

1218-510: The public, and at least 200 acres (0.81 km ) in extent. In 1926, profiting from the agricultural depression, the ideal place was found: Hall Farm, near Whipsnade village , was derelict, and held almost 600 acres (2.4 km ) on the Chiltern Hills . ZSL bought the farm in December 1926 for £13,480 12s 10d. In 1928 the first animals arrived at the new Whipsnade Park —two Amherst pheasants ,

1260-439: The revival of their population. Researchers are looking towards induced pluripotent stem cells (iPSC), as they hold limitless possibilities. With the lack of natural mating occurring within the species due to the limited number of them, this sub-species provides researchers the opportunity for iPSC intervention. Other methods, including artificial insemination with fresh semen (AI), have been used successfully in another sub-species,

1302-472: The uterus of a domestic house cat, resulting in a healthy male wildcat. The Frozen Ark is a frozen zoo established in 2004 and jointly managed by the Zoological Society of London , the London Natural History Museum , and the University of Nottingham . This organization operates as a charity with many different departments including the DNA laboratory, consortium, taxon expert groups, and

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1344-404: The very low temperatures required, varying levels of stress are put on the DNA samples. Spermatozoa , in particular, are stressed by temperature shock, osmotic stress, and oxidative stress with the latter being the most detrimental. When temperature shock occurs, the membrane is damaged through freezing and thawing of the sperm. Osmotic stress occurs when ice crystals form inside the nucleus during

1386-516: The wild. Its current director is Michael W. Bruford ( Cardiff University ). The Frozen Ark was a finalist for the Saatchi & Saatchi Award for World Changing Ideas in 2006. The project was founded by Ann Clarke , her husband Bryan Clarke and Dame Anne McLaren . Since Bryan Clarke's death in 2014, the Frozen Ark's interim director has been Mike Bruford . Frozen zoo The first frozen zoo

1428-563: The world's first cloned black-footed ferret was born. This ferret, named Elizabeth Ann , marked the first time a U.S. endangered species was successfully cloned. The cells of two 1980s wild-caught black-footed ferrets that never bred in captivity were preserved in the San Diego Wildlife Alliance Frozen Zoo. One of them was cloned to increase genetic diversity in this species in December 2020. More clones of both are planned. They will initially be bred separately from

1470-506: Was a Przewalski's horse stallion named Kuporovic, born in the UK in 1975, and relocated three years later to the US, where he died in 1998. Due to concerns over the loss of genetic variation in the captive Przewalski's horse population, and in anticipation of the development of new cloning techniques, tissue from the stallion was cryopreserved at the San Diego Zoo's Frozen Zoo. Breeding of this individual in

1512-485: Was born on 1 April 2003, and the second two days later. The second was euthanized , apparently suffering from large offspring syndrome (an overgrowth disorder ), but the first survived and lived for seven years at the San Diego Zoo, where it died in April 2010 after it broke a leg and was euthanized. In 2020, the first cloned Przewalski's horse was born, the result of a collaboration between San Diego Zoo Global , ViaGen Equine and Revive & Restore . The cloning

1554-425: Was carried out by somatic cell nuclear transfer (SCNT), whereby a viable embryo is created by transplanting the DNA -containing nucleus of a somatic cell into an immature egg cell ( oocyte ) that has had its own nucleus removed, producing offspring genetically identical to the somatic cell donor. Since the oocyte used was from a domestic horse, this was an example of interspecies SCNT. The somatic cell donor

1596-429: Was criticised as inadequately researched by Peter Chalmers Mitchell in 1929; both histories were labelled inaccurate by John Bastin in 1970. As the twentieth century began, the need to maintain and research large animals in a more natural environment became clear. Peter Chalmers Mitchell (ZSL Secretary 1903–35) conceived the vision of a new park no more than 70 miles (110 km) away from London and thus accessible to

1638-512: Was established at the San Diego Zoo by pathologist Kurt Benirschke in 1972. At the time there was no technology available to make use of the collection, but Benirschke believed such technology would be developed in the future. The frozen zoo idea was later supported in Gregory Benford 's 1992 paper proposing a Library of Life. Zoos such as the San Diego Zoo and research programs such as

1680-744: Was replaced with a partnership with the University of Cambridge. ZSL runs London Zoo , Whipsnade Zoo and had planned to open an aquarium, Biota! . The society published the Zoological Record ( ZR ) from 1864 to 1980, when the ZR was transferred to BIOSIS . The Society has published the Proceedings of the Zoological Society of London , now called the Journal of Zoology , since 1830. Since 1998 it has also published Animal Conservation . Other publications include

1722-553: Was succeeded by the Marquess of Lansdowne who supervised the building of the first animal houses, a parcel of land in Regent's Park having already been obtained from the Crown at the inaugural meeting. It received a royal charter from George IV on 27 March 1829. The purpose of the society was to create a collection of animals for study at leisure, an associated museum and library. In April 1828,

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1764-507: Was then implanted in a domestic cow ( Bos taurus ). On 8 January 2001, the gaur, named Noah, was born in Sioux Center, Iowa . Noah was initially healthy, but the next day, he came down with clostridial enteritis, and died of dysentery within 48 hours of birth. This is not uncommon in uncloned animals, and the researchers did not think it was due to the cloning. The banteng was the second endangered species to be successfully cloned , and

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