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Entomophthorales

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A conidium ( / k ə ˈ n ɪ d i ə m , k oʊ -/ kə- NID -ee-əm, koh- ; pl. : conidia ), sometimes termed an asexual chlamydospore or chlamydoconidium ( pl. : chlamydoconidia ), is an asexual , non- motile spore of a fungus . The word conidium comes from the Ancient Greek word for dust , κόνις ( kónis ). They are also called mitospores due to the way they are generated through the cellular process of mitosis . They are produced exogenously. The two new haploid cells are genetically identical to the haploid parent, and can develop into new organisms if conditions are favorable, and serve in biological dispersal .

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22-442: Ancylistaceae Completoriaceae Entomophthoraceae Meristacraceae Neozygitaceae The Entomophthorales are an order of fungi that were previously classified in the class Zygomycetes . A new subdivision, Entomophthoromycotina , in 2007, was circumscribed for them. Most species of the entomophthorales are pathogens of insects . A few attack nematodes , mites , and tardigrades , and some (particularly species of

44-404: A common mold, specifically is of interest. Aspergillus is not only a familiar fungus found across various different settings in the world, but it poses a danger for immunocompromised individuals, as inhaled Aspergillus conidia could germinate inside the respiratory tract and cause aspergillosis, a form of pulmonary infection, and continual developments of aspergillosis such as new risk groups and

66-509: A smaller version of the original spore, or (in some species) an adhesive spore elevated on a very slender conidiophore called a capilliconidiophore . Debates have centred on whether the Basidiobolaceae should be included in the entomophthorales, or raised to ordinal status. Molecular systematics approaches so far give an ambiguous answer. Some analyses suggest the Basidiobolaceae are more closely related to certain chytrid fungi than to

88-424: Is Conidiobolus coronatus , which is typically found in soils and parasitizing termites and aphids but has been known to infect mammals . Conidia Asexual reproduction in ascomycetes (the phylum Ascomycota ) is by the formation of conidia, which are borne on specialized stalks called conidiophores . The morphology of these specialized conidiophores is often distinctive between species and, before

110-427: Is coenocytic or irregularly septate . The nuclei are small. During interphase , condense chromatin is absent, but a central nucleolus can be observed. The mycelium can become disjointed. In Ancylistes , who are parasites of desmids (algae form), the hyphae grow toward the ends of the host. As the hyphae grow, branches that follow the groves between plates in the chloroplast are produced. Upon reaching

132-582: Is known to cause hypersensitivity pneumonitis , an occupational hazard for forest workers and paper mill employees. Conidia are often the method by which some normally harmless but heat-tolerating (thermotolerant), common fungi establish infection in certain types of severely immunocompromised patients (usually acute leukemia patients on induction chemotherapy, AIDS patients with superimposed B-cell lymphoma, bone marrow transplantation patients (taking immunosuppressants), or major organ transplant patients with graft versus host disease ). Their immune system

154-407: The development of molecular techniques at the end of the 20th century, was widely used for identification of ( e.g. Metarhizium ) species. The terms microconidia and macroconidia are sometimes used. There are two main types of conidium development: A conidium may form germ tubes (germination tubes) and/or conidial anastomosis tubes (CATs) in specific conditions. These two are some of

176-514: The dormant conidia are dictated by a few central regulatory proteins, which are the main drivers of the conidia and conidiophore formation. One of these proteins, the developmental regulatory protein wetA, has been found to be particularly essential; in wetA-defective mutants have reduced tolerance to external factors mentioned above, and exhibit weak synthesization of the conidial cell wall. In addition to these central regulators, some notable groups of genes/proteins include other regulatory proteins like

198-491: The dormant conidia is shed and the growth of the hyphae cells begins, which has a significantly different composition compared to the dormant conidia cell. Breaking of dormancy involves transcription, but not translation; protein synthesis inhibitors prevent isotropic growth, while DNA and RNA synthesis inhibitors do not, and the start of breaking of dormancy is accompanied by and increase in transcripts for genes for biosynthesis of proteins, and immediate protein synthesis. Following

220-455: The end, the hyphae grow toward the other end to completely encircle the chloroplast. Septa are produced progressively with segments containing many nuclei. Conidia are produced singly on unbranched sporophores . Like other members of Entomophthorales , conidia are forcibly discharged, which occurs through papillar eversion . Zygospores are formed along the axis of conjugation and can be angular in shape. Sexual reproduction results in

242-533: The entomophthorales. and place it within the Chytridiales order. Others find weak support to maintain them in the Entomophthorales. Morphological characters can be found to support either hypothesis. Ancylistaceae The Ancylistaceae are a family of fungi in the order Entomophthorales . The family currently contains 3 genera: Ancylistes , Macrobiotophthora , Conidiobolus . Capillidium

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264-448: The expansion of the cell via isotropic growth, studies have observed many new proteins emerging from the processes in the breaking of dormancy and transcripts associated with remodeling of the cell wall, suggesting that remodeling of the cell wall is a central process during isotropic growth. In the polarized growth stage, upregulated and overexpressed proteins and transcripts included ones involved in synthesis of chitin (a major component of

286-549: The formation of a zygospore that functions as a resting spore . The zygospore is formed by the fusion of gametangial cells or the scalariform fusion of hyphae. Little is known about the zygospores. The genus Ancylistes are parasites of desmids, a group of green algae . Macrobiotophthora are parasites of tardigrades and nematodes . The genus Conidiobolus are common saprobes and occasional parasites of vertebrates or insects. Capillidium are also parasites of vertebrates or insects. The most well known member

308-424: The fungal cell wall), mitosis and DNA processing, remodeling of cell morphology, and ones in germ tube formation pertaining to infection and virulence factors. Conidiogenesis is an important mechanism of spread of plant pathogens. In some cases, specialized macroscopic fruiting structures perhaps 1 mm or so in diameter containing masses of conidia are formed under the skin of the host plant and then erupt through

330-537: The genus Conidiobolus ) are free-living saprotrophs . The name "entomophthorales" is derived from the Ancient Greek for insect destroyer ( entomo- = referring to insects, and phthor = "destruction"). Named after genus Entomophthora in 1856. Most species of the entomophthorales produce ballistic asexual spores that are forcibly discharged. When not landing on a suitable host, these spores can germinate to make one of several alternate spore forms, including

352-620: The resistance against antifungal drugs. Germination in Aspergillus follows a sequence of three different stages: dormancy, isotropic growth, and polarized growth. The dormant conidia are able to germinate even after an year of remaining at room temperature, due to their resilient intracellular and extracellular characteristics, which enable them to undergo harsh conditions like dehydration, variation in osmotic pressure, oxidation, and temperature, and change in UV exposure and acidity levels. These abilities of

374-746: The specialized hyphae that are formed by fungal conidia. The germ tubes will grow to form the hyphae and fungal mycelia . The conidial anastomosis tubes are morphologically and physiologically distinct from germ tubes. After conidia are induced to form conidial anastomosis tubes, they grow homing toward each other, and they fuse. Once fusion happens, the nuclei can pass through fused CATs. These are events of fungal vegetative growth and not sexual reproduction. Fusion between these cells seems to be important for some fungi during early stages of colony establishment. The production of these cells has been suggested to occur in 73 different species of fungi. As evidenced by recent literature, conidia germination of Aspergillus ,

396-486: The surface, allowing the spores to be distributed by wind and rain. One of these structures is called a conidioma (plural: conidiomata ). Two important types of conidiomata, distinguished by their form, are: Pycnidial conidiomata or pycnidia form in the fungal tissue itself, and are shaped like a bulging vase. The conidia are released through a small opening at the apex, the ostiole . Acervular conidiomata, or acervuli , are cushion-like structures that form within

418-443: The swelling from isotropic growth directs the growth to one side of the cell, and leads to the formation of a germ tube. First, however, the conidia must go through the stage of breaking dormancy. In some species of Aspergillus, dormancy is broken when the dormant conidia is introduced to a carbon source in the presence of water and air, while in other species, the mere presence of glucose is enough to trigger it. The dense outer layer of

440-509: The tissues of a host organism: Mostly they develop a flat layer of relatively short conidiophores which then produce masses of spores. The increasing pressure leads to the splitting of the epidermis and cuticle and allows release of the conidia from the tissue. Conidia are always present in the air, but levels fluctuate from day to day and with the seasons. An average person inhales at least 40 conidia per hour. Exposure to conidia from certain species, such as those of Cryptostroma corticale ,

462-403: The velvet regulator proteins, which contribute to fungal growth, and other molecules that target specific unfavorable intra and extracellular conditions, like heat shock proteins. The phases following dormancy include isotropic growth, in which increased intracellular osmotic pressure and water uptake causes swelling of the conidia and increased cellular diameter, and polarized growth, in which

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484-515: Was added in 2020, it was once thought to be a sub-genus of Conidiobolus . Microconidiobolus and Neoconidiobolus were also added in 2020. This family was originally an order thought to be included with the aquatic Phycomycetes and included members of Oomycota . It was Helen Berdan , who determined that Ancylistes belonged to order Entomophthorales . Later, the Oomycetes were removed as were several zygomycete genera. The mycelium

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