Activity-based proteomics , or activity-based protein profiling ( ABPP ) is a functional proteomic technology that uses chemical probes that react with mechanistically related classes of enzymes.
5-516: ABPP may stand for: Activity-based protein profiling , a proteomic technology to monitor enzyme activity Agile business process platform , a main product area of i2 Technologies American Battlefield Protection Program , a United States federal government program under the purview of the National Park Service American Board of Professional Psychology Topics referred to by
10-541: A specially designed electrophile that becomes covalently -linked to a nucleophilic residue in the active site of an active enzyme . An enzyme that is inhibited or post-translationally modified will not react with an activity-based probe. The tag may be either a reporter such as a fluorophore or an affinity label such as biotin or an alkyne or azide for use with the Huisgen 1,3-dipolar cycloaddition (also known as click chemistry ). A major advantage of ABPP
15-406: Is different from Wikidata All article disambiguation pages All disambiguation pages Activity-based protein profiling The basic unit of ABPP is the probe, which typically consists of two elements: a reactive group (RG, sometimes called a "warhead") and a tag. Additionally, some probes may contain a binding group which enhances selectivity. The reactive group usually contains
20-613: Is the ability to monitor the availability of the enzyme active site directly, rather than being limited to protein or mRNA abundance. With classes of enzymes such as the serine hydrolases and metalloproteases that often interact with endogenous inhibitors or that exist as inactive zymogens , this technique offers a valuable advantage over traditional techniques that rely on abundance rather than activity. Furthermore, ABPP could be used to target specific proteins which were previously viewed as undruggable targets. In recent years ABPP has been combined with tandem mass spectrometry enabling
25-405: The same term [REDACTED] This disambiguation page lists articles associated with the title ABPP . If an internal link led you here, you may wish to change the link to point directly to the intended article. Retrieved from " https://en.wikipedia.org/w/index.php?title=ABPP&oldid=1145508544 " Category : Disambiguation pages Hidden categories: Short description
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